Convenient Structural Characterization of Intact Glycosphingolipids by MALDI-TOF Mass Spectrometry with Increased Laser Power and Cooling Gas Flow

Suzuki, Minoru

doi:10.1007/978-4-431-77924-7_12

Minoru Suzuki⁸

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Abstract

Matrix-assisted laser desorption/ionization quadrupole ion trap time-of-flight mass spectrometry (MALDI-QIT-TOF MS) was applied to the structural characterization of intact glycosphingolipids. Lithium adduct ions of glycosphingolipids were analyzed using MALDI-QIT-TOF MS (Suzuki et al. 2005) under strong conditions of increased laser power and cooling gas flow. Consequently, the fragmentation patterns of MS and MS² for the glycosphingolipids under the strong conditions were comparable to those of MS² and MS³ obtained under standard conditions, respectively (Suzuki et al. 2006).

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References

Suzuki Y, Suzuki M, Ito E, Ishii H, Miseki K, Suzuki A (2005) Convenient and rapid analysis of linkage isomers of fucose-containing oligosaccharides by matrix-assisted laser desorption/ionization quadrupole ion trap time-of-flight mass spectrometry. Glycoconjugate J 22:427–431
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Suzuki Y, Suzuki M, Ito E, Goto-Inoue N, Miseki K, Iida J, Yamazaki Y, Yamada M, Suzuki A (2006) Convenient structural analysis of glycosphingolipids using MALDI-QIT-TOF mass spectrometry with increased laser power and cooling gas flow. J Boichem 139:771–777
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Authors and Affiliations

Sphingolipid Expression Laboratory, RIKEN Frontier Research System, 2-1 Hirosawa, Wako, Saitama, 351-1098, Japan
Minoru Suzuki

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Minoru Suzuki
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Editors and Affiliations

Department of Disease Glycomics, Research Institute for Microbial Diseases, Osaka University, 1-1 Yamadaoka, Suita, Osaka, 565-0871, Japan
Naoyuki Taniguchi M.D., Ph.D. (Professor) (Professor)
Systems Glycobiology Group, Advanced Science Institute, RIKEN, Wako, 2-1 Hirosawa, Wako, Saitama, 351-0198, Japan
Naoyuki Taniguchi M.D., Ph.D. (Professor) (Professor)
Institute of Glycotechnology, Future Science and Technology Joint Research Center, Tokai University, Hiratsuka, Kanagawa, 259-1292, Japan
Akemi Suzuki M.D., Ph.D. (Professor) (Professor)
RIKEN Discovery Research Institute, Synthetic Cellular Chemistry Laboratory, 2-1 Hirosawa, Wako, Saitama, 351-0198, Japan
Yukishige Ito Ph.D. (Chief Researcher) (Chief Researcher)
Research Center for Medical Glycoscience, Advanced Industrial Science and Technology, AIST Tsukuba, Central 2, Tsukuba, Ibaraki, 305-8568, Japan
Hisashi Narimatsu M.D., Ph.D.
Research Center for Glycobiotechnology, Ritsumeikan University, 1-1-1 Noji-Higashi, Kusatsu, Shiga, 525-8577, Japan
Toshisuke Kawasaki Ph.D. (Professor) (Professor)
Graduate School of Science, Osaka University, 1-1 Machikaneyama, Toyonaka, Osaka, 560-0043, Japan
Sumihiro Hase Ph.D. (Professor) (Professor)

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Suzuki, M. (2008). Convenient Structural Characterization of Intact Glycosphingolipids by MALDI-TOF Mass Spectrometry with Increased Laser Power and Cooling Gas Flow. In: Taniguchi, N., Suzuki, A., Ito, Y., Narimatsu, H., Kawasaki, T., Hase, S. (eds) Experimental Glycoscience. Springer, Tokyo. https://doi.org/10.1007/978-4-431-77924-7_12

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DOI: https://doi.org/10.1007/978-4-431-77924-7_12
Publisher Name: Springer, Tokyo
Print ISBN: 978-4-431-77923-0
Online ISBN: 978-4-431-77924-7
eBook Packages: Biomedical and Life SciencesBiomedical and Life Sciences (R0)

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