Skip to main content
SpringerLink
Log in

Cell Therapy for Regeneration of Spinal Ganglion Neurons

  • Chapter
  • First Online:
Regenerative Medicine for the Inner Ear

  • 1000 Accesses

Abstract

Cell transplantation is regarded as one of the potential therapies to replace diseased auditory neurons and experimental studies have been compiled to revive auditory neurons during the last decade. Revitalizing auditory neurons are crucial to accomplish rewiring of peripheral auditory system in toto as they are essential for the survival and maintenance of cochlear nucleus cells. We reviewed the relevant literature with the object of functional replacement of auditory neurons. After many types of cell lines have been investigated as donor cells so far, now our studies should be directed to conclusively clarify which cell line(s) are most suitable as donor cells and when the optimal differentiation stage is to harvest donor cells. Cell transplantation site is critical for successful cell transplantation and should be selected so that growth factors are efficiently available for cell survival and differentiation. Auditory nerve is a favorable transplantation site as neurotrophins synthesized in hair cells directly flow down into auditory nerve trunk. Total replacement of auditory neurons might be ideal but currently a distant goal. However, it is likely adding even a small number of auditory neurons to existing residual neurons would be clinically significant to patients’ hearing.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Log in via an institution
Chapter
USD 29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 89.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 119.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD 169.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

References

  1. Sekiya T, Kojima K, Matsumoto M, Ito J. Replacement of diseased auditory neurons by cell transplantation. Front Biosci. 2008;13:2165–76.

    Article  CAS  PubMed  Google Scholar 

  2. Altschuler RA, O’Shea KS, Miller JM. Stem cell transplantation for auditory nerve replacement. Hear Res. 2008;242(1–2):110–6.

    Google Scholar 

  3. Shi F, Edge AS. Prospects for replacement of auditory neurons by stem cells. Hear Res. 2013;297:106–12.

    Google Scholar 

  4. Ito J, Kojima K, Kawaguchi S. Survival of neural stem cells in the cochlea. Acta Otolaryngol. 2001;121(2):140–2.

    Article  CAS  PubMed  Google Scholar 

  5. Needham K, Minter RL, Shepherd RK, Nayagam BA. Challenges for stem cells to functionally repair the damaged auditory nerve. Expert Opin Biol Ther. 2013;13(1):85–101. doi:10.1517/14712598.2013.728583.

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  6. Sekiya T, Holley MC, Kojima K, Matsumoto M, Helyer R, Ito J. Transplantation of conditionally immortal auditory neuroblasts to the auditory nerve. Eur J Neurosci. 2007;25(8):2307–18. doi:10.1111/j.1460-9568.2007.05478.x.

    Article  PubMed  Google Scholar 

  7. Reyes JH, O’Shea KS, Wys NL, Velkey JM, Prieskorn DM, Wesolowski K, et al. Glutamatergic neuronal differentiation of mouse embryonic stem cells after transient expression of neurogenin 1 and treatment with BDNF and GDNF: in vitro and in vivo studies. J Neurosci. 2008;28(48):12622–31. doi:10.1523/JNEUROSCI.0563-08.2008.

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  8. Chen W, Jongkamonwiwat N, Abbas L, Eshtan SJ, Johnson SL, Kuhn S, et al. Restoration of auditory evoked responses by human ES-cell-derived otic progenitors. Nature. 2012;490(7419):278–82.

    Google Scholar 

  9. MacLaren RE, Pearson RA, MacNeil A, Douglas RH, Salt TE, Akimoto M, et al. Retinal repair by transplantation of photoreceptor precursors. Nature. 2006;444(7116):203–7.

    Article  CAS  PubMed  Google Scholar 

  10. Sekiya T, Kojima K, Matsumoto M, Kim TS, Tamura T, Ito J. Cell transplantation to the auditory nerve and cochlear duct. Exp Neurol. 2006;198(1):12–24.

    Article  PubMed  Google Scholar 

  11. Nishimura K, Nakagawa T, Ono K, Ogita H, Sakamoto T, Yamamoto N, et al. Transplantation of mouse induced pluripotent stem cells into the cochlea. Neuroreport. 2009;20(14):1250–4. doi: 10.1097/Wnr.0b013e32832ff287.

  12. Nishimura K, Nakagawa T, Sakamoto T, Ito J. Fates of murine pluripotent stem cell-derived neural progenitors following transplantation into mouse cochleae. Cell Transplant. 2012;21(4):763–71. doi:10.3727/096368911X623907.

    Article  PubMed  Google Scholar 

  13. Hu Z, Andang M, Ni D, Ulfendahl M. Neural cograft stimulates the survival and differentiation of embryonic stem cells in the adult mammalian auditory system. Brain Res. 2005;1051(1–2):137–44. doi:10.1016/j.brainres.2005.06.016.

    Article  CAS  PubMed  Google Scholar 

  14. Hu Z, Ulfendahl M, Olivius NP. NGF stimulates extensive neurite outgrowth from implanted dorsal root ganglion neurons following transplantation into the adult rat inner ear. Neurobiol Dis. 2005;18(1):184–92. doi:10.1016/j.nbd.2004.09.010.

    Article  CAS  PubMed  Google Scholar 

  15. Hara A, Salt AN, Thalmann R. Perilymph composition in scala tympani of the cochlea: influence of cerebrospinal fluid. Hear Res. 1989;42(2–3):265–71.

    CAS  PubMed  Google Scholar 

  16. Okano T, Nakagawa T, Endo T, Kim TS, Kita T, Tamura T, et al. Engraftment of embryonic stem cell-derived neurons into the cochlear modiolus. Neuroreport. 2005;16(17):1919–22.

    Article  PubMed  Google Scholar 

  17. Corrales CE, Pan L, Li H, Liberman MC, Heller S, Edge AS. Engraftment and differentiation of embryonic stem cell-derived neural progenitor cells in the cochlear nerve trunk: growth of processes into the organ of Corti. J Neurobiol. 2006;66(13):1489–500. doi:10.1002/neu.20310.

    Article  PubMed Central  PubMed  Google Scholar 

  18. Shi F, Corrales CE, Liberman MC, Edge AS. BMP4 induction of sensory neurons from human embryonic stem cells and reinnervation of sensory epithelium. Eur J Neurosci. 2007;26(11):3016–23. doi:10.1111/j.1460-9568.2007.05909.x.

    Article  PubMed  Google Scholar 

  19. Ogita H, Nakagawa T, Sakamoto T, Inaoka T, Ito J. Transplantation of bone marrow-derived neurospheres into guinea pig cochlea. Laryngoscope. 2010;120(3):576–81. doi:10.1002/lary.20776.

    Article  PubMed  Google Scholar 

  20. Palmgren B, Jin Z, Jiao Y, Kostyszyn B, Olivius P. Horseradish peroxidase dye tracing and embryonic statoacoustic ganglion cell transplantation in the rat auditory nerve trunk. Brain Res. 2011;1377:41–9.

    Google Scholar 

  21. Hu Z, Ulfendahl M, Olivius NP. Central migration of neuronal tissue and embryonic stem cells following transplantation along the adult auditory nerve. Brain Res. 2004;1026(1):68–73.

    Google Scholar 

  22. Regala C, Duan M, Zou J, Salminen M, Olivius P. Xenografted fetal dorsal root ganglion, embryonic stem cell and adult neural stem cell survival following implantation into the adult vestibulocochlear nerve. Exp Neurol. 2005;193(2):326–33.

    Article  CAS  PubMed  Google Scholar 

  23. Minor LB. Labyrinthine fistulae: pathobiology and management. Curr Opin Otolaryngol Head Neck Surg. 2003;11(5):340–6.

    Article  PubMed  Google Scholar 

  24. Thonabulsombat C, Johansson S, Spenger C, Ulfendahl M, Olivius P. Implanted embryonic sensory neurons project axons toward adult auditory brainstem neurons in roller drum and Stoppini co-cultures. Brain Res. 2007;1170:48–58. doi:10.1016/j.brainres.2007.06.085.

    Article  CAS  PubMed  Google Scholar 

  25. Rubel EW, Fritzsch B. Auditory system development: primary auditory neurons and their targets. Annu Rev Neurosci. 2002;25:51–101.

    Article  CAS  PubMed  Google Scholar 

  26. Ryugo DK, Kretzmer EA, Niparko JK. Restoration of auditory nerve synapses in cats by cochlear implants. Science. 2005;310(5753):1490–2. doi:10.1126/science.1119419.

    Article  CAS  PubMed  Google Scholar 

  27. Abematsu M, Tsujimura K, Yamano M, Saito M, Kohno K, Kohyama J, et al. Neurons derived from transplanted neural stem cells restore disrupted neuronal circuitry in a mouse model of spinal cord injury. J Clin Invest. 2010;120(9):3255–66.

    Google Scholar 

  28. Fayad JN, Linthicum Jr FH. Multichannel cochlear implants: relation of histopathology to performance. Laryngoscope. 2006;116(8):1310–20.

    Article  PubMed  Google Scholar 

  29. Linthicum Jr FH, Fayad J, Otto SR, Galey FR, House WF. Cochlear implant histopathology. Am J Otol. 1991;12(4):245–311.

    PubMed  Google Scholar 

  30. Nadol Jr JB, Shiao JY, Burgess BJ, Ketten DR, Eddington DK, Gantz BJ, et al. Histopathology of cochlear implants in humans. Ann Otol Rhinol Laryngol. 2001;110(9):883–91.

    PubMed  Google Scholar 

  31. Khan AM, Handzel O, Burgess BJ, Damian D, Eddington DK, Nadol Jr JB. Is word recognition correlated with the number of surviving spiral ganglion cells and electrode insertion depth in human subjects with cochlear implants? Laryngoscope. 2005;115(4):672–7.

    Article  PubMed  Google Scholar 

  32. Nathan PW, Smith MC, Deacon P. The corticospinal tracts in man. Course and location of fibres at different segmental levels. Brain. 1990;113(Pt 2):303–24.

    Article  PubMed  Google Scholar 

  33. Blight AR. Cellular morphology of chronic spinal cord injury in the cat: analysis of myelinated axons by line-sampling. Neuroscience. 1983;10(2):521–43.

    Article  CAS  PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Otolaryngology-Head and Neck Surgery, Graduate School of Medicine, Kyoto University, Kyoto, 606-8507, Japan

    Tetsuji Sekiya & Masaaki Ishikawa

Authors
  1. Tetsuji Sekiya
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Masaaki Ishikawa
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Tetsuji Sekiya .

Editor information

Editors and Affiliations

  1. Department of Otolaryngology-Head and Neck Surgery, Kyoto University Graduate School of Medicine, Sakyo-ku, Kyoto, Japan

    Juichi Ito

Rights and permissions

Reprints and permissions

Copyright information

© 2014 Springer Japan

About this chapter

Cite this chapter

Sekiya, T., Ishikawa, M. (2014). Cell Therapy for Regeneration of Spinal Ganglion Neurons. In: Ito, J. (eds) Regenerative Medicine for the Inner Ear. Springer, Tokyo. https://doi.org/10.1007/978-4-431-54862-1_28

Download citation

  • DOI: https://doi.org/10.1007/978-4-431-54862-1_28

  • Published:

  • Publisher Name: Springer, Tokyo

  • Print ISBN: 978-4-431-54861-4

  • Online ISBN: 978-4-431-54862-1

  • eBook Packages: MedicineMedicine (R0)

Publish with us

Policies and ethics

Search

Navigation