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Integrated Vascular Engineering: Vascularization of Reconstructed Tissue

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Vascular Engineering

Abstract

In this chapter, we describe culture methods to construct microvascular networks as well as approaches to integrating capillary networks with 3D epithelial tissue-engineered constructs. First, culture models of microvascular networks such as in vitro angiogenesis and vasculogenesis models are introduced. Using these culture models, the roles of endothelial cells (ECs), such as endothelial tip, stalk, and phalanx cells, are demonstrated. Additionally, regulatory factors, including both biochemical and biophysical factors, are discussed in the context of 3D capillary formation, including the process of vascular development, growth, and maturation. Next, we focus on the use of microfluidics technologies for investigating capillary morphogenesis. Examples of 3D capillary formation assays with growth factor gradients and different extracellular matrix materials are described. Cocultures of ECs and the other cell types in microfluidic devices are also introduced to show the potential of microfluidic vascular formation models. The vascularization of constructed tissues is discussed from the viewpoints of horizontal and vertical approaches for combining capillary structures and epithelial tissues in vitro. Finally, the concept of integrated vascular engineering and future perspectives are discussed.

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Acknowledgements

This work was partially supported by Grants-in-Aid for Scientific Research (25282135, 25560208, 25249018) from the Japan Society for Promotions of Science, and by the Human Resources Program in Energy Technology of the KETEP grant from the Ministry of Trade, Industry & Energy, Republic of Korea. (No. 20124010203250).

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Authors and Affiliations

  1. Department of System Design Engineering, Keio University, 3-14-1 Hiyoshi, Kohoku-ku, Yokohama, 223-8522, Japan

    Ryo Sudo & Kazuo Tanishita

  2. School of Mechanical Engineering, Korea University, 145 Anam-ro, Seongbuk-gu, Seoul, 02841, Korea

    Seok Chung

  3. Department of Mechanical Engineering, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, MA, 02139, USA

    Yoojin Shin

Authors
  1. Ryo Sudo
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  2. Seok Chung
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  3. Yoojin Shin
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  4. Kazuo Tanishita
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Corresponding author

Correspondence to Ryo Sudo .

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Editors and Affiliations

  1. Waseda University, Tokyo, Tokyo, Japan

    Kazuo Tanishita

  2. The University of Tokyo, Tokyo, Tokyo, Japan

    Kimiko Yamamoto

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Sudo, R., Chung, S., Shin, Y., Tanishita, K. (2016). Integrated Vascular Engineering: Vascularization of Reconstructed Tissue. In: Tanishita, K., Yamamoto, K. (eds) Vascular Engineering. Springer, Tokyo. https://doi.org/10.1007/978-4-431-54801-0_16

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  • DOI: https://doi.org/10.1007/978-4-431-54801-0_16

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