Abstract
In recent years, insights into the role of the epicardium in cardiac development have significantly changed. An important contribution to this increasing knowledge comes from the availability of mouse models that facilitate the study of the fate of the epicardial cell lineage and that allow epicardial-specific manipulation of expression of genes involved in regulation of epicardial cell behavior. In this contribution we will discuss our growing understanding of the role of the epicardium and epicardially derived cells in the formation of the atrioventricular valve leaflets. We will illustrate how epicardially derived cells specifically contribute to the development of the leaflets that derive from the lateral atrioventricular cushions, and we will discuss the role of Bmp signaling, through the Bmp receptor BmpR1A/Alk3, in the regulation of the preferentially migration of EPDCs into the parietal AV valve leaflets.
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1 Introduction
1.1 The AV Valves and Their Leaflets
The atrioventricular (AV) valves are importa nt cardiac components that, when properly formed, prevent the retrograde flow of blood through the AV junction during ventricular systole. The precursor tissues of the AV valves are the endocardial AV cushions, formed by (1) the accumulation of cardiac jelly in the AV canal followed by (2) the population of these cushions by endocardially derived cells (ENDCs) resulting from an endocardial epithelial-to-mesenchymal transformation (or endoEMT) [1]. Not all AV cushions develop at the same time. The two “major” AV cushions (the inferior and superior cushion) form first, while the two “lateral” cushions form later [2, 3]. The major AV cushions will eventually fuse together. They will also fuse with the mesenchymal cap of the primary atrial septum and the dorsal mesenchymal protrusion (DMP) to form the AV mesenchymal complex [4]. This process is essential in the partition of the left and right atrial and ventricular components. The AV mesenchymal complex is also involved in the formation of the septal leaflet of the right AV valve and the aortic (or anterior) leaflet of the left AV valve [4, 5]. The lateral AV cushions are significantly smaller than the major AV cushions and develop at the lateral myocardial AV junctions. Just like the major AV cushions, the lateral AV cushions become populated with ENDCs. The right lateral cushion eventually forms the parietal leaflet of the right AV valve, while the left lateral cushion forms the parietal (or mural/posterior) leaflet of the left AV valve (Fig. 20.1).
1.2 The Epicardium and Epicardially Derived Cells (EPDCs)
In the mouse, epicardial development starts around embryonic day 9.5 (ED9.5) when the proepicardium, a heterogeneous cluster of cells, can be seen as a “cauliflower-like” mesothelial structure at the inferior margin of the cardiac sinus venosus at the venous pole of the heart. Around ED10, proepicardial cells reach and attach to the myocardium. Subsequently, they migrate out over the surface of the developing heart, ultimately covering the heart entirely. As a result of an epicardial epithelial-to-mesenchymal transition (epiEMT), epicardially derived cells (EPDC) are generated that migrate into the sub-epicardial space and into the underlying myocardium. Over the last 15 years or so, a series of cell fate studies in avian and murine models have been published reporting that EPDCs can, or have the potential to, differentiate in multiple cell types including coronary smooth muscle cells, interstitial fibroblasts , coronary endothelium, and potentially cardiomyocytes [6–9]. It is important to note that the contribution of EPDCs to the coronary endothelium and myocardium remains controversial.
1.3 The Contribution of EPDCs to the Developing AV Valves
In recent papers we have discussed the cascade of epicardial-related events involved in the formation and development of the AV junction [7, 10]. This process starts with the establishment of the AV-epicardium. After the formation of the AV-epicardium, subsequent AV-epiEMT leads to the generation of AV-EPDCs, a process regulated by a variety of factors. These AV-EPDCs eventually form the AV sulcus, which can be seen as a mesenchymal wedge between the expanding atrial and ventricular myocardial walls. As AV-EPDCs from the sulcus start to penetrate the AV myocardial junction, the formation of the annulus fibrosus is initiated. The annulus fibrosus is a fibrous tissue plane that physically and electrically separates the atrial from the ventricular working myocardium. From the annulus fibrosus, a subset of AV-EPDCs continues to migrate into the parietal AV valve leaflets, where they eventually comprise a large percentage of the mesenchymal cells found of the leaflet (Fig. 20.2). Just like the ENDCs found in the developing leaflets, the AV-EPDCs in the valves eventually become valve interstitial cells (VICs). It is important to note that very few AV-EPDCs are found in the leaflets that are developing from the fused major AV cushions [7].
2 The Role of Bmp S ignaling in Regulating the Contribution of EPDC to the AV Valves
2.1 Epicardial-Specific Deletion of the Bmp Receptor BmpR1A/Alk3 Leads to Disruption of AV Junction Development
To elucidate the mechanisms that regulate the events that are responsible for the contribution of AV-EPDCs to the tissues of the AV junction, we investigated the importance of bone morphogenetic protein (Bmp) signaling in the epicard ium. Bmp isoforms are known to be involved in a variety of developmental steps in heart development. In particular Bmp2, found at high levels in the AV myocardium, has been shown to be important for the development of the AV cushions by promoting endoEMT [11]. In our study, we determined that Bmp4 is highly expressed in the AV-epicardium and in the AV-EPDCs that form the AV sulcus. Furthermore, strong phospho-Smad-1/5/8 labeling of the AV-EPDCs indicates that canonical Bmp signaling is active in these cells, strongly suggesting that this signaling pathway is important in regulating the events associated with the cascade of epicardial-related events at t he AV junction [12]. To test the hypothesis that Bmp signaling is important for the development of the structures that rely on the contribution of AV-EPDCs, we deleted the Bmp r eceptor Alk3 from the epicardial and epicardially derived cells using an epicardial-specific cre-mouse (the Wt1-cre mouse [7, 12]). This approach resulted in a decrease in the size of the AV sulcus, an inhibition of the formation of the annulus fibrosus, and a reduction of the number of EPDCs that migrate into the parietal AV valve leaflets. Remarkably, electrophysiological analysis of postnatal Wt1-cre;Alk3 mice did not show, despite the defect in the formation of the annulus fibrosus, ventricular pre-excitation [12]. In addition, and also quite unexpectedly, postnatal Wt1-Cre;Alk3 showed a myxomatous mitral valve phenotype, particularly of the left parietal leaflet (Fig. 20.3).
2.2 Discussion
Our studies convincingly show that Bmp signaling plays a critical role in the establishment of the mesenchymal and fibrous tissues at the AV ju nction. We propose that during normal development, AV-epiEMT is a crucial process as it is responsible for generating the critical amount of AV-EPDCs needed for the subsequent formation of the annulus fibrosus and the associated migration of AV-EPDCs into the parietal leaflets. We believe that perturbation of Bmp signaling by conditionally deleting Alk3 from the epicardial cell population inhibits AV-epiEMT resulting in a domino effect leading to (1) inhibition of AV sulcus formation, (2) failure of the annulus fibrosus to form, and (3) reduction of the number of EPDCs that migrate into the AV valve leaflets (Fig. 20.4).
2.3 Future Direction and Clinical Implications
Myxomatous valve disease is generally considered an acquired disease, often observed in patients with mitral valve prolapse. The observed myxomatous changes in the leaflets of the postnatal Wt1-cre;Alk3 mice, however, are associated with a reduced influx of EPDCs within those leaflets during embryonic development. We therefore propose that perturbation in the norm al development of EPDCs in the AV junction might play the role in the etiology of myxomatous valve degen eration in human heart disease.
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Acknowledgments
The authors would like to acknowledge the financial support by NIH grants P30 GM103342 (A.W.), UL1RR029882 and UL1TR000062 (A.W.), R01HL033756 (A.W.) American Heart Association Grant-in-Aid 13GRNT16220004 (A.W.), American Heart Association Predoctoral grant 12PRE11340000 (M.M.L.), European Community’s Sixth Framework Program Grant LSHM-CT-2005-018630 (MJBvdH), and Netherlands Heart Foundation Grant 1996M002 (MJBvdH).
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Lockhart, M.M., van den Hoff, M., Wessels, A. (2016). The Role of the Epicardium in the Formation of the Cardiac Valves in the Mouse. In: Nakanishi, T., Markwald, R., Baldwin, H., Keller, B., Srivastava, D., Yamagishi, H. (eds) Etiology and Morphogenesis of Congenital Heart Disease. Springer, Tokyo. https://doi.org/10.1007/978-4-431-54628-3_20
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