Summary
The role of white blood cells in acute cerebral disorders such as ischemia or stroke is still unclear. Therefore, in the present study we investigated the effects of the leukotaxin n-formyl-methionyl-leucylphenylalanine (fMLP) on white blood cell endothelial-cell interactions in the rat brain surface microcirculation. An improved closed cranial window technique5 was applied. Superfusion of fMLP in rising concentrations (10−8– 10−5M) was seen to induce rolling and adherence of leukocytes to the endothelium of small venules. Rolling was more effectively stimulated than firm attachment. fMLP-induced vasodilation was more pronounced in arterioles than in venules. In this study it has been shown that the hydrophilic fMLP is effectively stimulating neutrophil chemotaxis across the blood-brain barrier. Further, the closed cranial window preparation is useful to analyze quantitatively properties of activated leukocytes, which may be pertinent in injury to the blood-brain barrier and induction of microcirculatory disturbances.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Engler RL, Schmid-Schönbein GW, Pavelec RL (1983) Leukocyte capillary plugging in myocardial ischemia and reperfusion in the dog. Am J Pathol 111: 98–111
Ernst E, Matrai A, Paulsen F (1987) Leukocyte rheology in recent stroke. Stroke 18: 59–62
Hallenbeck JM, Dutka AJ, Tanishima R, Kochanek PM, Kumaroo KK, Thompson CB, Obrenovitch TP, Contreras TJ (1986) Polymorphonuclear leukocyte accumulation in brain regions with low blood flow during the early postischemic period. Stroke 17: 246–253
Harlan JM (1985) Leukocyte-endothelial interactions. Blood 65 (3): 513–525
Kawamura S, Schürer L, Goetz A, Kempski O, Schmucker B, Baethmann A (1990) An improved closed cranial window tech-nique for investigation of blood-brain barrier function and cerebral vasomotor control in the rat. Int J Microcirc: Clin Exp (in press)
Lewis RE, Miller RA, Granger HJ (1989) Acute microvascular effects of the chemotactic peptide N-formyl-methionyl-leucylphenylalanine: Comparisons with leukotriene B4. Microvasc Res 37: 53–69
Nagai K, Katori M (1988) Possible changes in the leukocyte membrane as a mechanism of leukocyte adhesion to the venular walls induced by leukotriene B4 and fMLP in the microvasculature of the hamster cheek pouch. Int J Microcirc: Clin Exp 7: 305–314
Nazar GB, Neal FK, Povlishock JT, Lee J, Hudson S (1988) Subarachnoid hemorrhage causes adherence of white blood cells to the cerebral arterial luminal surface. In: Wilkins Re (ed) Cerebral vasospasm. Raven Press, New York, pp 343–356
Unterberg A, Wahl M, Hammersen F, Baethmann A (1987) Permeability and vasomotor response of cerebral vessels during exposure to arachidonic acid. Acta Neuropathol (Berl) 73: 209–219
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1990 Springer-Verlag
About this paper
Cite this paper
Corvin, S., Schürer, L., Abels, C., Kempski, O., Baethmann, A. (1990). Effect of Stimulation of Leukocyte Chemotaxis by fMLP on White Blood Cell Behaviour in the Microcirculation of Rat Brain. In: Reulen, HJ., Baethmann, A., Fenstermacher, J., Marmarou, A., Spatz, M. (eds) Brain Edema VIII. Acta Neurochirurgica, vol 51. Springer, Vienna. https://doi.org/10.1007/978-3-7091-9115-6_19
Download citation
DOI: https://doi.org/10.1007/978-3-7091-9115-6_19
Publisher Name: Springer, Vienna
Print ISBN: 978-3-7091-9117-0
Online ISBN: 978-3-7091-9115-6
eBook Packages: Springer Book Archive