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The significance of the anther tapetum in the biochemistry of pollen pigmentation — an overview

  • Conference paper
The Tapetum

Part of the book series: Plant Systematics and Evolution ((SYSTEMATICS,volume 7))

Abstract

Flavonoids such as chalcones, flavonol di-and triglycosides as well as anthocyanins are essentially involved in pollen pigmentation. Phenylalanine ammonia-lyase (PAL) and chalcone synthase (CHS) represent two key enzymes in the biosynthetic pathway of flavonoids. In enzymatical studies the distribution of the two enzymes in anthers of Tulipa cv. ‘Apeldoorn’ was analysed. After separation of the loculus material in a pollen and a tapetum fraction the highest enzyme activities of both PAL and CHS were found in the tapetum fraction whereas the pollen fraction showed only low enzyme activities. This was confirmed by immunohistochemical studies using antibodies against CHS. The CHS was present in the tapetum and epidermal cells of anthers at an early and middle postmeiotic developmental stage. The results imply that the tapetum cells play a crucial role in the flavonoid biosynthesis in the loculus of the anthers and consequently in the pollen pigmentation.

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References

  • Amrhein, N., Zenk, M. H., 1971: Untersuchungen zur Rolle der Phenylalanin-AmmoniumLyase (PAL) bei der Regulation der Flavonoidsynthese im Buchweizen (Fagopyrum esculentum Moench). — Z. Pflanzenphysiol. 64: 145–168.

    CAS  Google Scholar 

  • Bäumker, P. A., Arendt, S., Wiermann, R., 1988: Metabolism of ferulic acid sucrose esters in anthers of Tulipa cv. Apeldoorn: I. The accumulation of esters and free sugars. — Z. Naturforsch. 43c: 641–646.

    Google Scholar 

  • Beerhues, L., Wiermann, R., 1988: Chalcone synthases from spinach (Spinacia oleracea L.). I. Purification, peptide patterns, and immunological properties of different forms. — Planta 173: 532–543.

    Article  CAS  Google Scholar 

  • Beerhues, L., Robenek, H., Wiermann, R., 1988: Chalcone synthases from spinach (Spinacia oleracea L.). II. Immunofluorescence and immunogold localization. — Planta 173: 544–553.

    Article  CAS  Google Scholar 

  • Beerhues, L., Forkmann, G., Schöpker, H., Stotz, G., Wiermann, R., 1989: Flavanone 3-hydroxylase and dihydroflavonol oxygenise activities in anthers of Tulipa. The significance of the tapetum fraction in flavonoid metabolism. — J. Pl. Physiol. 133: 743–746.

    Article  CAS  Google Scholar 

  • Bradford, M. M., 1976: A rapid and sensitive method for quantitation of microgram quantities of protein utilizing the principle of protein dye binding. — Anal. Biochem. 72: 248–254.

    Article  PubMed  CAS  Google Scholar 

  • Coe, E. H., McCormlac, S. M., Modena, S. A., 1981: White pollen in maize. — J. Heredity 72: 318–320.

    Google Scholar 

  • Fey, H., 1972: Eriochrome Black, a means for reduction of nonspecificity in immunofluorescence. — Path. Microbiol. 38: 271–277.

    CAS  Google Scholar 

  • Gubatz, S., Herminghaus, S., Meurer, B., Strack, D., Wiermann, R., 1986: The location of hydroxycinnamic acid amides in the exine of Corylus pollen. — Pollen & Spores 28: 347–354.

    Google Scholar 

  • Heller, W., Forkmann, G., 1988: Biosynthesis. — In Harborne, J. B., (Ed.): The flavonoids, pp. 399–425. — London: Chapman and Hall.

    Google Scholar 

  • Herdt, E., Sütfeld, R., Wiermann, R., 1978: The occurrence of enzymes involved in phenylpropanoid metabolism in the tapetum fraction of anthers. — Europ. J. Cell Biol. 17: 433–441.

    CAS  Google Scholar 

  • Hrazdina, G., Marx, G. A., Hoch, H. C., 1982: Distribution of secondary plant metabolites and their biosynthetic enzymes in pea (Pisum sativum L.) leaves. — Pl. Physiol. 70: 745–748.

    Article  CAS  Google Scholar 

  • Jahnen, W., Hahlbrock, K., 1988: Differential regulation and tissue-specific distribution of enzymes of phenylpropanoid pathways in developing parsley seedlings. — Planta 173: 453–458.

    Article  CAS  Google Scholar 

  • Koes, R. E., Blokland, R. van, Quattrocchio, F., Tunen, A. J. van, Mol, J. N. M., 1990: Chalcone synthase promoters in Petunia are active in pigmented and unpigmented cell types. — Pl. Cell 2: 379–392.

    CAS  Google Scholar 

  • Lowry, O. H., Rosebrough, N. J., Farr, A. L., Randall, R. J., 1951: Protein measurement with the folin phenol reagent. - J. Biol. Chem. 193: 265–275.

    PubMed  CAS  Google Scholar 

  • Meer, I. M. van der, Stam, M. E., Tunen, A. J. van, Mol, J. N. M., Stuitje, A. R., 1992: Antisense inhibition of flavonoid biosynthesis in Petunia anthers results in male sterility. — Pl. Cell 4: 253–262.

    Google Scholar 

  • Rittscher, M., Wiermann, R., 1983: Occurrence of phenylalanine ammonia-lyase (PAL) in isolated tapetum cells of Tulipa anthers. — Protoplasma 118: 219–224.

    Article  CAS  Google Scholar 

  • Schächtele, C., Steup, M., 1986: α-1,4 glucan phosphorylase forms from leaves of spinach (Spinacia oleracea L.). I. In situ localization by indirect immunofluorescence. — Planta 167: 444–451.

    Article  Google Scholar 

  • Schmelzer, E., Jahnen, W., Hahlbrock, K., 1988: In situ localization of light-induced chalcone synthase mRNA, chalcone synthase, and flavonoid end products in epidermal cells of parsley leaves. — Proc. Natl. Acad. Sci. USA 85: 2989–2993.

    Article  PubMed  CAS  Google Scholar 

  • Stanley, R. G., Linskens, H. F., 1974: Pollen: biology, biochemistry, management. — Berlin, Heidelberg, New York: Springer.

    Google Scholar 

  • Strack, D., Sachs, G., Wiermann, R., 1981: Pollen of Tulipa cv. Apeldoorn as an accumulation site of flavonol di-and triglycosides. — Z. Pflanzenphysiol. 103: 291–296.

    CAS  Google Scholar 

  • Styles, E. D., Ceska, O., 1981: Genotypes affecting the flavonoid constituents of maize pollen. — Maydica 26: 141–152.

    CAS  Google Scholar 

  • Wiermann, R., 1981: Secondary plant products and cell and tissue differentiation. — In Stumpf, P. K., Conn, E. E., (Eds): The biochemistry of plants. 7: Secondary plant products, pp. 85–116. — New York, London, Toronto, Sydney, San Francisco: Academic Press.

    Google Scholar 

  • Wiermann, R., Vieth, K., 1983: Outer pollen wall, an important accumulation site for flavonoids. — Protoplasma 118: 230–233.

    Article  CAS  Google Scholar 

  • Wiermann, R., Gubatz, S., 1992: Pollen wall and sporopollenin. — Int. Rev. Cytol. 140: 35–72.

    Article  CAS  Google Scholar 

  • Zerback, R., Dressler, K., Hess, D., 1989: Flavonoid compounds from pollen and stigma of Petunia hybrida: Inducers of the vir region of the Agrobacterium tumefaciens Ti plasmid. — Pl. Sci. 62: 83–91.

    Article  CAS  Google Scholar 

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Author information

Authors and Affiliations

  1. Institut für Pharmazeutische Biologie, Universität Bonn, Nussallee 6, D-53115, Bonn, Federal Republic of Germany

    L. Beerhues

  2. Institut für Botanik und Botanischer Garten, Universität Münster, Schlossgarten 3, D-48149, Münster, Federal Republic of Germany

    M. Rittscher, H. Schöpker, C. Schwerdtfeger & R. Wiermann

Authors
  1. L. Beerhues
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  2. M. Rittscher
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  3. H. Schöpker
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  4. C. Schwerdtfeger
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  5. R. Wiermann
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Editor information

Editors and Affiliations

  1. Institut für Botanik und Botanischer Garten, Universität Wien, Austria

    M. Hesse

  2. Dipartimento di Biologia Ambientale, Universitá degli Studi di Siena, Italy

    E. Pacini

  3. Department of Plant Cytology and Morphology, Agricultural University Wageningen, The Netherlands

    M. Willemse

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© 1993 Springer-Verlag Wien

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Beerhues, L., Rittscher, M., Schöpker, H., Schwerdtfeger, C., Wiermann, R. (1993). The significance of the anther tapetum in the biochemistry of pollen pigmentation — an overview. In: Hesse, M., Pacini, E., Willemse, M. (eds) The Tapetum. Plant Systematics and Evolution, vol 7. Springer, Vienna. https://doi.org/10.1007/978-3-7091-6661-1_11

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  • DOI: https://doi.org/10.1007/978-3-7091-6661-1_11

  • Publisher Name: Springer, Vienna

  • Print ISBN: 978-3-7091-7373-2

  • Online ISBN: 978-3-7091-6661-1

  • eBook Packages: Springer Book Archive

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