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The in-vitro conservation of valuable genetic resources

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Plant Tissue Culture

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Abstract

Today tissue culture techniques are indispensable tools for modern plant research. They provided fascinating new methods for fundamental research, particularly for investigations of cell metabolism like the elucidation of biochemical pathways or the investigation of subcellular transport mechanisms. They even led to the discovery of phenomena like somaclonal variation that became new topics of investigation. At the same time cell culture techniques contributed considerably to plant biotechnology. The fermentation of suspension cultures and the mass propagation of in-vitro plants have been made possible Improvement of old and the development of new breeding techniques were achieved as well as techniques like virus eradication Finally, even the most advanced techniques of molecular transformation of plants were based on tissue culture techniques.

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References

  • Abernethy D., Conner J.(1992) Laboratory guide for the micropropagation of asparagus.Crop & Food Research Rep 1: 34.

    Google Scholar 

  • Bajaj Y. P. S. (1977) Initiation of shoots and callus from potato sprouts and axillary buds frozen at —196°C. Crop. Imp. 4: 48–53.

    Google Scholar 

  • Bajaj Y. P. S. (1977) Clonal multiplication and cryopresevation of cassava through tissue culture. Crop. Imp. 4: 198–204.

    Google Scholar 

  • Bajaj Y. P. S. (1981) Regeneration of plants from potato meristems freeze-preserved for 24 months. Euphytica 30: 141–145.

    Article  Google Scholar 

  • Bouafia S., Jelti N., Lairy G., Blanc A., Bonnel E., Dereuddre J. (1996) Cryopreservation of potato shoot tips by encapsulation-dehydration. Potato Res. 39: 69–78.

    Article  Google Scholar 

  • Fabre J., Dreuddre J. (1990) Encapsulation-dehydration: A new approach to cryopreservation of Solanum shoot tips. Cryo-Lett. 11: 413–426.

    Google Scholar 

  • Fletcher P. J., Fletcher J. D., Cross R. J. (1998) Potato germplasm: in-vitro stoarge and virus reduction. New Zealand J Crop. and Hort. Sci. 26: 249–252.

    Article  Google Scholar 

  • Fukai S., Goi M., Tanaka M. (1994) The chimeric structure of the apical dome of Chrysanthemum ((Dendranthema grandiflorum (Ramat.) Kitam.) is affected by cryopreservation. Scientia Hort. 57: 347–351.

    Article  Google Scholar 

  • Grout B. W. W., Henshaw G. G. (1978) Freeze preservation of potato shoot tip cultures. Ann. Bot. (London) 42: 1227–1230.

    Google Scholar 

  • Grout B. W. W., Westcott R., Henshaw G. G. (1978) Survival of shoot meristems of tomato seedlings frozen in liquid nitrogen. Cryobiology 15(4): 478–483.

    Article  PubMed  CAS  Google Scholar 

  • Grout B. W. W. (1990) In-vitro conservation of germplasm. In: Bhojwani S. S. (ed.) Plant tissue culture: Application and limitation. Elsevier, Amsterdam Tokyo 19: 394–411.

    Google Scholar 

  • Haberlandt G. (1902) Kulturversuche mit isolierten Pflanzenzellen. Sitzungsber. Akad. Wiss. Math. Naturwiss. Kl. (Wien) 11: 69–92.

    Google Scholar 

  • Hirai D., Sakai A. (1999) Cryopreservation of in-vitro-grown meristems of potato (Solanum tuberosum L.) by encapsulation-vitrification. Potato Res. 42: 153–160.

    Article  Google Scholar 

  • Hitmi A., Barthomeuf C., Sallanon H. (1999) Cryopreservation of Chrysanthemum cinerariaefolium shoot tips. Effects of pretreatment conditions and retention of biosynthetic capacity. Cryo-Lett. 20: 113–120.

    CAS  Google Scholar 

  • Kartha K. K., Leung N. L., Gamborg O. L. (1979) Freeze-preservation of pea meristems in liquid nitrogen and subsequent plant regeneration. Plant Sci. Lett. 15: 7–24.

    Google Scholar 

  • Kartha K. K., Leung N. L., Pahl K. (1980) Cryopreservation of strawberry meristems and mass propagation of plantlets. J. Am. Soc. Hortic. Sci. 105: 481–484.

    CAS  Google Scholar 

  • Kohmura H., Sakai A., Chokyu S., Yakuwa T. (1992) Cryopreservation of in-vitro cultured multiple bud cluster of asparagus (Asparagus officinalis L. cv. Hiroshimagreen, 2n = 30 = by the techniques of vitrification. Plant Cell Rep. 11: 433–437.

    Article  Google Scholar 

  • Maruyama E., Kinoshita I., Ishii K., Ohba K., Sakai A. (1997) Germplasm conservation of Guazuma crinita a useful tree in the Peru-Amazon, by the cryopreservation of in-vitro-cultured multiple bud clusters. Plant Cell Tissue Organ Cult. 48: 161–165.

    Article  Google Scholar 

  • Mix-Wagner G. (1996) Management of the in-vitro genebank for old potato cultivars at the Institute of Crop Science in Braunschweig. Landbauforsch Völkenrode 46: 5–9.

    Google Scholar 

  • Mix-Wagner G., Conner A. J., Cross R. J. (2000) Survival and recovery of asparagus shoot tips after cryopreservation using the „droplet” method. New Zealand J Crop. and Hort. Sci. 28: 283–287.

    Article  Google Scholar 

  • Mix-Wagner G. (1999) The conservation of potato cultivars. Potato Res. 42: 427–436.

    Article  Google Scholar 

  • Mix-Wagner G., Schumacher H. M., Cross R. J. (2002) Recovery of frozen potatoapices after several years of storage in liquid nitrogen. (in preparation).

    Google Scholar 

  • Murashige T., Skoog F. (1962) A revised medium for rapid growth and bioassays in tobacco tissue culture. Physiol. Plant 16: 473-493.

    Google Scholar 

  • Nag K. K., Street H. E. (1973) Carrot embryogenesis from frozen cell cultures. Nature (London) 245: 270–272.

    Article  Google Scholar 

  • Ogawa R., Ishikawa M., Niwata E., Oosawa K. (1997) Cryopreservation of shoot primordia cultures of melon using a slow prefreezing procedure. Plant Cell Tissue Organ Cult. 49: 171–177.

    Article  CAS  Google Scholar 

  • Paul H., Daigny G., Sangwan-Norreel B. S. (2000) Cryopreservation of apple (Malus domestica Borkh.) shoot tips following encapsulation-dehydration or encapsulation-vitrification. Plant Cell Rep. 19: 768-774.

    Article  CAS  Google Scholar 

  • Quatrano R. S. (1968) Freeze-preservation of cultured flax utilizing DMSO. Plant Physiol. 43: 2057–2061.

    Article  PubMed  CAS  Google Scholar 

  • Sakai A. (1956) Survival of plant tissue of super-low temperature. Contrib. Inst. Temp. Sci. Haikkaido Univ. Ser. B 14: 17.

    Google Scholar 

  • Sakai A. (1960) Survival of the twigs of woody plants at —196°C. Nature (London) 185: 392.

    Google Scholar 

  • Schäfer-Menuhr A., Müller E., Mix-Wagner G. (1996) Cryopreservation: an alternative for the long-term storage of old potato varieties. Potato Res. 39: 507–513.

    Article  Google Scholar 

  • Schäfer-Menuhr A., Schumacher H. M., Mix-Wagner G. (1997) Cryopreservation of potato cultivars — design of a method for routine application in genebanks. Acta Horticulturae 447: 477–482.

    Google Scholar 

  • Schnabel-Preikstas B., Earle E. D., Steponkus R (1992) Cryopreservation of potato shoot tips by vitrification. Abstract of the 29th Annual Meeting of the Society for Cryobiology, 48.

    Google Scholar 

  • Towill L. E. (1981) Survival at low temperatures of shoot-tips of cultivars of Solanum tuberosum group Tuberosum. Cryo. Lett. 2: 373–382.

    Google Scholar 

  • Towill L. E., Jarret R. L. (1992) Cryopreservation of sweet potato (Ipomea batatas L. Lam.) shoot tips by vitrification. Plant Cell Rep. 11: 175–178.

    Google Scholar 

  • Withers L. (1980) Tissue culture storage for genetic conservation. IBPGR Technical Rep 8: 1–91.

    Google Scholar 

  • Zhao Y., Wu Y., Engelmann F., Zhou M., Chen S. (1999) Cryopreservation of apple in-vitro shoot tips by the droplet freezing method. Cryo-Lett. 20: 109–112.

    Google Scholar 

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Author information

Authors and Affiliations

  1. Institute of Crop and Grassland Science, Federal Agricultural Research Centre (FAL), Bundesallee, D-38116, Braunschweig, Germany

    Gunda Mix-Wagner

  2. DSMZ — Deutsche Sammlung von Mikroorganismen, Mascheroder Weg 1b, D-38124, Braunschweig, Germany

    Heinz Martin Schumacher

  3. Zellkulturen GmbH, Mascheroder Weg 1b, D-38124, Braunschweig, Germany

    Heinz Martin Schumacher

Authors
  1. Gunda Mix-Wagner
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  2. Heinz Martin Schumacher
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Editor information

Editors and Affiliations

  1. Institut für Angewandte Mikrobiologie, Universität für Bodenkultur Wien, Österreich, Nußdorfer Lände 11, A-1190, Wien, Österreich

    Margit Laimer

  2. Institut für Ökologie und Naturschutz, Universität Wien, Österreich, Althanstrasse 14, A-1090, Wien, Österreich

    Waltraud Rücker

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© 2003 Springer-Verlag Wien

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Mix-Wagner, G., Schumacher, H.M. (2003). The in-vitro conservation of valuable genetic resources. In: Laimer, M., Rücker, W. (eds) Plant Tissue Culture. Springer, Vienna. https://doi.org/10.1007/978-3-7091-6040-4_8

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  • DOI: https://doi.org/10.1007/978-3-7091-6040-4_8

  • Publisher Name: Springer, Vienna

  • Print ISBN: 978-3-211-83839-6

  • Online ISBN: 978-3-7091-6040-4

  • eBook Packages: Springer Book Archive

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