Skip to main content

Micropropagation of ornamental plants

  • Chapter
Plant Tissue Culture

Abstract

When Gottlieb Haberlandt started culturing isolated plant cells in artificial nutrient media he was mainly interested in cell to cell relationships within complex multicellular organisms. Discussing the results of his experiments he pointed out that inspite of remarkable cell growth, cell division was never observed (Haberlandt 1902). He speculated that “growth enzymes” obviously were necessary for stimulating cell division, and he recommended the addition of extracts from meristem tissue (“Vegetationsspitzen”) to the nutrient solutions. He stressed as well the use of embryo sac fluid. Haberlandt expected that the method of cultivating isolated plant cells would permit investigations of important problems from a new experimental approach. At that time he did not consider that cell culture could be useful for commercial propagation of plants.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

eBook
USD 16.99
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 109.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  • Aitken-Christie J., Kozai T., Takayama S. (1995) Automation in plant tissue culture — general introduction and overview. In: Aitken-Christie J., Kozai T., Smith M. A. L. (eds.) Automation and environmental control in plant tissue cultures. Kluwer Academic Publishers, Dordrecht, pp. 1–18.

    Google Scholar 

  • Bajaj Y. P. S. (1995) Preface. In: Bajaj Y. P. S. (ed.) Biotechnology in Agriculture and Forestry 30 — Somatic embryogenesis and synthetic seed I. Springer, Berlin.

    Google Scholar 

  • Bouman H., Kuijpers A. M., Hol. T., Schoo W. (2001) Differences in growth and regeneration of suspension cultures derived from various callus lines of Cyclamen persicum cv. `Purple Flamed’. In: Schwenkel H. G. (ed.) Reproduction of Cyclamen persicum Mill. through somatic embryogenesis using suspension culture systems. COST Action 822, European Commission, Brussels, EUR 19697, pp. 46–54.

    Google Scholar 

  • Brandau K., Preil W., Lieberei R. (1997) Differences in compounds released by embryogenic and non-embryogenic suspension cultures of Euphorbia pulcherrima. Biologia Plantarum 39: 113–124.

    Article  CAS  Google Scholar 

  • Brown D. C. W., Finstad K. I., Watson E. M. (1995) Somatic embryogenesis in herbaceous dicots. In: Thorpe T. A. (ed.) In vitro embryogenesis in plants. Kluwer Academic Publishers, Dordrecht, pp. 345–416.

    Chapter  Google Scholar 

  • Buiatti M., Gimelli F. (1993) Somaclonal variation in ornamentals. In: Schiva T., Mercuri A. (eds.) Creating genetic variation in ornamentals. Proc. XVII`h EUCARPIA Symposium, San Remo, pp. 5–24.

    Google Scholar 

  • Capellades Queralt M., Beruto M., Vanderschaeghe A., Debergh R. C. (1991) Ornamentals. In: Debergh R. C., Zimmerman R. (eds.) Micropropagation. Kluwer Academic Publishers, Dordrecht, pp. 215–229.

    Chapter  Google Scholar 

  • Cassells A. C. (1991) Problems in tissue culture: culture contamination. In: Debergh R. C., Zimmerman R. H. (eds.) Micropropagation. Kluwer Academic Publishers, Dordrecht, pp. 31–44.

    Chapter  Google Scholar 

  • Cervelli R., Senaratna T. (1995) Economic aspects of somatic embryogenesis. In: Aitken-Christie J., Kozai T., Smith M. A. L. (eds.) Automation and environmental control in plant tissue cultures. Kluwer Academic Publishers, Dordrecht, pp. 29–64.

    Google Scholar 

  • Chu I. Y. E., Kurtz S. L. (1990) Commercialisation of plant micropropagation. In: Ammirato R. V., Evans D. R., Sharp W. R., Bajaj Y. R. S. (eds.) Handbook of plant cell culture, Vol. 5, Ornamental species. McGraw-Hill Publishing Company, New York, pp. 126–164.

    Google Scholar 

  • Debergh P. C., Read R. E. (1991) Micropropagation. In: Debergh R. C., Zimmerman R. H. (eds.) Micropropagation. Kluwer Academic Publishers, Dordrecht, pp. 1–13.

    Chapter  Google Scholar 

  • Dillen W., Dijkstra I., Oud J. (1996) Shoot regeneration in long-term callus cultures derived from mature flowering plants of Cyclamen persicum Mill. Plant Cell Rep. 15: 545–548.

    Article  CAS  Google Scholar 

  • Dudits D., Györgyey J., Bögre L., Bako L. (1995) Molecular biology of somatic embryogenesis. In: Thorpe T. A. (ed.) In vitro embryogenesis in plants. Kluwer Academic Publishers, Dordrecht, pp. 267–308.

    Chapter  Google Scholar 

  • Dunstan D. I., Tautorus T. E., Thorpe T. A. (1995) Somatic embryogenesis in woody plants. In: Thorpe T. A. (ed.) In vitro embryogenesis in plants. Kluwer Academic Publisher, Dordrecht, pp. 471–538.

    Chapter  Google Scholar 

  • Geier T., Kohlenbach H. W., Reuther G. (1979) Klonale Vermehrung von Cyclamen persicum Mill. durch Gewebekultur. Gartenbauwissenschaft 44: 226–237.

    CAS  Google Scholar 

  • Grotkass D., Lieberei R., Preil W. (1995) Polyphenoloxidase — activity in embryogenic and non-embryogenic suspension cultures of Euphorbia pulcherrima. Plant Cell Rep. 14: 428–431.

    Article  CAS  Google Scholar 

  • Haberlandt G. (1902) Kulturversuche mit isolierten Pflanzenzellen. Sitz. ber. Akad. Wiss. Wien, math.-naturwiss. Kl. 3: 69–92.

    Google Scholar 

  • Hoffmann M., Preil W. (1987) In vitro Kultur von Blattexplantaten verschiedener Cyclamen Idiotypen. Gartenbauwissenschaft 52: 145–148.

    Google Scholar 

  • Hohe A., Winkelmann T., Schwenkel H. G. (1999a) The effect of oxygen partial pressure in bioreactors on cell proliferation and subsequent differentiation of somatic embryos of Cyclamen persicum. Plant Cell Tissue and Organ Culture 59: 39–45.

    Article  CAS  Google Scholar 

  • Hohe A., Winkelmann T., Schwenkel H. G. (1999b) CO2 accumulation in bioreactor suspension cultures of Cyclamen persicum Mill. and its effect on cell growth and regeneration of somatic embryos. Plant Cell Rep. 18: 863–867.

    Article  CAS  Google Scholar 

  • Hohe A., Winkelmann T., Schwenkel H. G. (2001) Development of somatic embryos of Cyclamen persicum Mill. in liquid culture. Gartenbauwissenschaft 66: 219–224.

    CAS  Google Scholar 

  • Hvoslef-Eide A. K., Munster C. (2001) Light quality effects on somatic embryogenesis of Cyclamen persicum Mill. in bioreactors. In: Schwenkel H. G. (ed.) Reproduction of Cyclamen persicum Mill. through somatic embryogenesis using suspension culture systems. COST Action 822, European Commission, Brussels. EUR 19697, pp. 79–84.

    Google Scholar 

  • Ibaraki Y., Kurata K. (2001) Automation of somatic embryo production. Plant Cell, Tissue and Organ Culture 59: 179–199.

    Article  Google Scholar 

  • Jones J. B. (1990) Economic considerations in tissue culture propagation of ornamentals. In: Ammirato P. V., Evans D. R., Sharp W. R., Bajaj Y. P. S. (eds.) Handbook of plant cell culture, Vol. 5, Ornamental species. McGraw-Hill Publishing Company, New York, pp. 165–178.

    Google Scholar 

  • Jones J. B., Sluis C. J. (1991) Marketing of micropropagated plants. In: Debergh P. C., Zimmerman R. H. (eds.) Micropropagation. Kluwer Academic Publishers, Dordrecht, pp. 141–154.

    Chapter  Google Scholar 

  • Komamine A., Matsumoto M., Tsu kahara M., Fujiwara A., Kawahara R., Ito M., Smith J., Nomura K., Fujimura T. (1990) Mechanisms of somatic embryogenesis in cell cultures — physiology, biochemistry and molecular biology. In: Nijkamp H. J. J., Van der Plas L. H. W., Van Aartrijk J. (eds.) Progress in plant cellular and molecular biology. Kluwer Academic Publishers, Dordrecht, pp. 307–313.

    Chapter  Google Scholar 

  • KrishnaRaj S., Vasil I. K. (1995) Somatic embryogenesis in herbaceous monocots. In: Thorpe T. A. (ed.) In vitro embryogenesis in plants. Kluwer Academic Publishers, Dordrecht, pp. 417–470.

    Chapter  Google Scholar 

  • Linnenbrink N., Preil W., Kraus L. (1983) Entwicklung einer in vitro-Kulturmethode für Arctostaphylos uva-ursi and A. nevadensis. Arch. Pharm 316: 97–100.

    Google Scholar 

  • Merkle S. A., Parrott W. A., Flinn B. S. (1995) Morphogenic aspects of somatic embryogenesis. In: Thorpe T. A. (ed.) In vitro embryogenesis in plants. Kluwer Academic Publishers, Dordrecht, pp. 155–204.

    Chapter  Google Scholar 

  • Morel G. M. (1960) Producing virus-free cymbidiums. Amer. Orchid. Soc. Bull 29: 495–497.

    Google Scholar 

  • Morel G. (1964) Tissue culture — A new means of clonal propagation in orchids. Amer. Orchid. Soc. Bull. 33: 473–478.

    Google Scholar 

  • Murashige T. (1974) Plant propagation through tissue cultures. Ann. Rev. Plant Physiol 25: 135–166.

    Article  CAS  Google Scholar 

  • Murashige T. (1978) The impact of plant tissue culture on agriculture. In: Thorpe T. A. (ed.) Frontiers of plant tissue culture. Proc. 4th IAPTC, Congress, Calgary, pp. 15–26.

    Google Scholar 

  • Murashige T., Huang L. C. (1987) Cloning plants by tissue culture: early years, current status and future prospects. Acta Horticulturae 212: 35–42.

    Google Scholar 

  • Nomura K., Komamine A. (1995) Physiological and biochemical aspects of somatic embryogenesis. In: Thorpe T. H. (ed.) In vitro embryogenesis in plants. Kluwer Academic Publishers, Dordrecht, pp. 249–266.

    Chapter  Google Scholar 

  • Osternack N., Saare-Surminski K., Preil W., Lieberei R. (1999) Induction of somatic embryos, adventitious shoots and roots in hypocotyl tissue of Euphorbia pulcherrima Willd. ex Klotzsch: comparative studies on embryogenic and organogenic competence. J. Applied Botany 73: 197–201.

    Google Scholar 

  • O’Riordain F. (1988) Directory of European Plant Tissue Culture Laboratories. COST Action 87. Commission of the European Communities, Brussels.

    Google Scholar 

  • O’Riordain F. (1991) Directory of European Plant Tissue Culture Laboratories 1990. COST Action 87. Commission of the European Communities, Brussels.

    Google Scholar 

  • O’Riordain F. (1994) Directory of European Plant Tissue Culture Laboratories 1993. COST Action 87. Commission of the European Communities, Brussels.

    Google Scholar 

  • O’Riordain F. (1999) Directory of European Plant Tissue Culture Laboratories 1996–1997. COST Action 822. Commission of the European Communities, Brussels, EUR 18833.

    Google Scholar 

  • Pierik R. L. M. (1987) In Vitro Culture of Higher Plants. Martinus Nijhoff Publishers, Dordrecht.

    Book  Google Scholar 

  • Pierik R. L. M. (1988) In vitro culture of higher plants as a tool in the propagation of horticultural crops. Acta Horticulturae 226: 25–40.

    Google Scholar 

  • Pierik R. L. M. (1990) Rejuvenation and micropropagation. In: Nijkampf H. J. J., Van der Plas L. H. W., Van Aartrijk J. (eds.) Progress in Plant Cellular and Molecular Biology. Kluwer Academic Publishers, Dordrecht, pp. 91–101.

    Chapter  Google Scholar 

  • Pierik R. L. M. (1991a) Micropropagation of ornamental plants. Acta Horticulturae 289: 45–53.

    Google Scholar 

  • Pierik R. L. M. (1991b) Commercial micropropagation in Western Europe and Israel. In: Debergh P. C., Zimmerman R. H. (eds.) Micropropagation. Kluwer Academic Publishers, Dordrecht, pp. 155–165.

    Chapter  Google Scholar 

  • Pierik R. L. M., Ruibing M. A. (1997) Developments in the micropropagation industry in The Netherlands. Plant Tissue Culture and Biotechnology 3: 152–153.

    Google Scholar 

  • Preil W. (1986) In vitro propagation and breeding of ornamental plants: advantages and disadvantages of variability. In: Horn W., Jensen C. J., Odenbach W., Schieder O. (eds.) Genetic Manipulation in Plant Breeding. Walter de Gruyter & Co, Berlin, pp. 377–403.

    Google Scholar 

  • Preil W., Beck A. (1991) Somatic embryogenesis in bioreactor culture. Acta Horticulturae 289: 179–192.

    Google Scholar 

  • Preil W., Krause I., Schneidereit M. (2001) Effects of carbon dioxide on growth of embryogenic Cyclamen persicum bioreactor cultures. In: Schwenkel H. G. (ed.) Reproduction of Cyclamen persicum Mill. through somatic embryogenesis using suspension culture systems. COST Action 822, European Commission, Brussels, EUR 19697, pp. 75–78.

    Google Scholar 

  • Puschel A. K., Winkelmann T., Schwenkel H. G. (2001) Studies on the inheritance of in vitro regeneration abilities in Cyclamen persicum Mill.: Analysis of breeding and F1 progenies. In: Schwenkel H. G. (ed.) Reproduction of Cyclamen persicum Mill. through somatic embryogenesis using suspension culture systems. COST Action 822, European Commission, Brussels, EUR 19697, pp. 107–114.

    Google Scholar 

  • Reinert J. (1958) Untersuchungen über die Morphogenese an Gewebekulturen. Ber. Dtsch. Bot. Ges. 71: 15.

    Google Scholar 

  • Sagawa Y., Kunisaki J. T. (1990) Micropropagation of floricultural crops. In: Ammirato P. V., Evans D. R., Sharp W. R., Bajaj Y. P. S. (eds.) Handbook of Plant Cell Culture, Vol. 5, Ornamental Species. McGraw-Hill Publishing Company, New York, pp. 25–56.

    Google Scholar 

  • Schwenkel H. G. (1991) Regeneration von Cyclamen persicum Mill. in vitro und deren Integration in die Cyclamenzüchtung. Thesis. University of Hannover.

    Google Scholar 

  • Schwenkel H. G. (2001) Development of a reproducible regeneration protocol for Cyclamen. In: Schwenkel H. G. (ed.) Reproduction of Cyclamen persicum Mill. through somatic embryogenesis using suspension culture systems. COST Action 822, European Commission, Brussels, EUR 19697, pp. 8–11.

    Google Scholar 

  • Skoog F., Miller C. O. (1957) Chemical regulation of growth and organ formation in plant tissues cultured in vitro. Symp. Soc. Exp. Biol. 11: 118–130.

    PubMed  CAS  Google Scholar 

  • Standaert de Metsenaere R. E. A. (1991) Economic considerations. In: Debergh P. C., Zimmerman R. H. (eds.) Micropropagation. Kluwer Academic Publishers, Dordrecht, pp. 123–140.

    Chapter  Google Scholar 

  • Steward F. C., Mapes M. O., Mears K. (1958) Growth and organised development of cultured cells. II. Organisation in cultures grown from freely suspended cells. Amer. J. Bot. 45: 705–708.

    Article  Google Scholar 

  • Swartz H. J. (1991) Post culture behavior: genetic and epigenetic effects and related problems. In: Debergh P. C., Zimmerman R. H. (eds.) Micropropagation. Kluwer Academic Publishers, Dordrecht, pp. 95–122.

    Chapter  Google Scholar 

  • Takayama S., Swedlund B., Miwa Y. (1991) Automated propagation of microbulbs of lilies. In: Vasil I. K. (ed.) Scale-up and automation in plant propagation. Academic Press. Inc., New York, pp. 112–132.

    Google Scholar 

  • Tisserat B., Esan E. B., Murashige T. (1979) Somatic embryogenesis in angiosperms. Hortic. Rev. 1: 1–78.

    Google Scholar 

  • Weber J., Preil W., Lieberei R. (1994) Somatic embryogenesis in bioreactor culture of Clematis tangutica. VIII`h International Congress for Plant Tissue and Cell Culture, Firenze/Italy, Book of Abstracts, p. 202.

    Google Scholar 

  • Winkelmann T., Hohe A., Schwenkel H. G. (1998) Establishing embryogenic suspension cultures in Cyclamen persicum `Purple Flamed’. Adv. Hort. Sci. 12: 25–30.

    Google Scholar 

  • Yeung E. C. (1995) Structural and developmental patterns in somatic embryogenesis. In: Thorpe T. A. (ed.) In vitro embryogenesis in plants. Kluwer Academic Publishers, Dordrecht, pp. 205–248.

    Chapter  Google Scholar 

  • Young P. S., Murthy H. N., Yoeup R. K. (2000) Mass multiplication of protocorm-like bodies using bioreactor system and subsequent plant regeneration in Phalaenopsis. Plant Cell, Tissue and Organ Culture 63: 67–72.

    Article  CAS  Google Scholar 

  • Ziv M. (1991) Vitrification: morphological and physiological disorders of in vitro plants. In: Debergh R. C., Zimmerman R. H. (eds.) Micropropagation. Kluwer Academic Publishers, Dordrecht, pp. 45–69.

    Chapter  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Editor information

Editors and Affiliations

Rights and permissions

Reprints and permissions

Copyright information

© 2003 Springer-Verlag Wien

About this chapter

Cite this chapter

Preil, W. (2003). Micropropagation of ornamental plants. In: Laimer, M., Rücker, W. (eds) Plant Tissue Culture. Springer, Vienna. https://doi.org/10.1007/978-3-7091-6040-4_7

Download citation

  • DOI: https://doi.org/10.1007/978-3-7091-6040-4_7

  • Publisher Name: Springer, Vienna

  • Print ISBN: 978-3-211-83839-6

  • Online ISBN: 978-3-7091-6040-4

  • eBook Packages: Springer Book Archive

Publish with us

Policies and ethics