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New Methods of Antigen Localization and Preservation in Paraffin Processed Tissues

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Progress in Surgical Pathology

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Abstract

THE DEVELOPMENT IN RECENT YEARS of relatively simple immunohistologic techniques for the demonstration of antigens in tissue sections has greatly aided pathologists in the routine diagnosis of many neoplastic conditions.1 Such investigations received further impetus with the advent of techniques for the production of monoclonal antibodies2 and with the increased availability of a wide range of antibodies reactive with various tumor types.3 The categorization of undifferentiated neoplasms, discrimination between reactive and neoplastic lymphoproliferative disorders and the classification of lymphomas has been particularly improved with these reagents.

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References

  1. Heyderman E: Tumour markers. In: Immunocytochemistry—Modern Methods and Applications, 2nd ed. Polak JM and van Noorden S (eds). Wright P.S.G., Bristol, 1986, 274–294.

    Google Scholar 

  2. Kohler G, Milstein C: Continuous cultures of fused cells secreting antibody of predefined specificity. Nature 256:495–497, 1975.

    Article  PubMed  CAS  Google Scholar 

  3. Gatter KC, Falini B, Mason DY: The use of monoclonal antibodies in histopathological diagnosis. In: Recent Advances in Histopathology. Vol. 12. Anthony PP and MacSween RNM (eds), Churchill Livingstone, Edinburgh, 1984, 36–67.

    Google Scholar 

  4. Giorno R: Technical considerations in immuno-histology of lymphoid cell membrane antigens. Surv Synth Pathol Res 3:165–188, 1984.

    PubMed  CAS  Google Scholar 

  5. Faulk WP, Taylor GM: An immunocolloid method for the electron microscope. Immunochemistry 8:1081–1083, 1971.

    Article  PubMed  CAS  Google Scholar 

  6. De Mey J: The preparation and use of gold probes. Colloidal gold probes in immunocytochemistry. In: Immunocytochemistry—Modern Methods and Applications, 2nd ed. Polak JM and van Noorden S (eds). Wright P.S.G., Bristol, 1986, 115–145.

    Google Scholar 

  7. Geoghegan WD, Scillian JJ, Ackerman GA: The detection of human B lymphocytes by both light and electron microscopy utilizing colloidal gold labelled anti-immunoglobulin. Immunol Comm 7:1–12, 1978.

    CAS  Google Scholar 

  8. De Mey J, Moeremans M, Geuens G, et al: Light and electron microscopic localization of calmodulin in mitotic cells. Eur J Cell Biol 22:297a, 1980.

    Google Scholar 

  9. Gu J, De Mey J, Moeremans M, et al: Sequential use of the PAP and immunogold staining methods for light microscopical double staining of tissue antigens. Its application to the study of regulatory peptides in the gut. Regulat Pept 1:356–374, 1981.

    Article  Google Scholar 

  10. De Mey J: Colloidal gold as marker and tracer in light and electron microscopy. Electron Microscopic Soc Am Bull 14:54–66, 1984.

    Google Scholar 

  11. Danscher G: Localization of gold in biological tissue. A photochemical method for light and electron microscopy. Histochemistry 71:81–88, 1981.

    Article  PubMed  CAS  Google Scholar 

  12. Holgate CS, Jackson P, Cowen PN, et al: Immunogold-silver staining: New method of immunostaining with enhanced sensitivity. J Histochem Cytochem 31:938–944, 1983.

    Article  PubMed  CAS  Google Scholar 

  13. Springall DR, Hacker GW, Grimelius L, et al: The potential of the immunogold-silver staining method for paraffin sections. Histochemistry 81:603–608, 1984.

    Article  PubMed  CAS  Google Scholar 

  14. Hacker GW, Springall DR, Van Noorden S, et al: The immunogold-silver staining method: A powerful tool in histopathology. Virchows Arch (Pathol Anat) 406:449–461, 1985.

    Article  CAS  Google Scholar 

  15. Scopsi L, Larsson L-I: Increased sensitivity in immunocytochemistry. Effects of double application of antibodies and of silver intensification on immunogold and peroxidase-antiperoxidase staining techniques. Histochemistry 82:321–329, 1985.

    Article  PubMed  CAS  Google Scholar 

  16. Slot JW, Geuze H: Size of protein A-colloidal gold probes for immuno-electron microscopy of ultrathin frozen tissue sections. J Cell Biol 90:533–536, 1981.

    Article  PubMed  CAS  Google Scholar 

  17. Lackie PM, Hennessy RJ, Hacker GW, et al: Investigation of immunogold-silver staining by electron microscopy. Histochemistry 83:545–550, 1985.

    Article  PubMed  CAS  Google Scholar 

  18. Moeremans M, Daneels G, Van Dyck A, et al: Sensitive visualization of antigen—antibody reactions in dot and blot immune overlay assays with immunogold and immunogold/silver staining. J Immunol Methods 74:353–360, 1984.

    Article  PubMed  CAS  Google Scholar 

  19. Manigley C, Roth J: Applications of immunocol-loids in light microscopy. IV. Use of photochemical silver staining in a simple and efficient double-staining technique. J Histochem Cytochem 33:1247–1251, 1985.

    Article  PubMed  CAS  Google Scholar 

  20. Holgate CS, Jackson P, Lauder I, et al: Surface membrane staining of immunoglobulins in paraffin sections of non-Hodgkin’s lymphomas using immunogold-silver staining technique. J Clin Pathol 36:742–746, 1983.

    Article  PubMed  CAS  Google Scholar 

  21. Sangster G, Crocker J, Nar P, et al: Benign and malignant (B cell) focal lymphoid aggregates in bone marrow trephines shown by means of an immunogold-silver technique. J Clin Pathol 39:453–457, 1986.

    Article  PubMed  CAS  Google Scholar 

  22. Morgan DR, Holgate CS, Dixon MF, et al: Primary small intestinal lymphoma: A study of 39 cases. J Pathol 147:211–221, 1985.

    Article  PubMed  CAS  Google Scholar 

  23. Teasdale J, Lewis FA, Barrett ID, et al: Immunocytochemical application of monoclonal antibodies to rat liver glucocorticoid receptor. J Pathol 150:227–237, 1986.

    Article  PubMed  CAS  Google Scholar 

  24. Poulsen HS, Ozzello L, King WJ, et al: The use of monoclonal antibodies to estrogen receptors (ER) for immunoperoxidase detection of ER in paraffin sections of human breast cancer tissue. J Histochem Cytochem 33:87–92, 1985.

    Article  PubMed  CAS  Google Scholar 

  25. Teasdale J, Jackson P, Holgate CS, Cowen PN: Identification of oestrogen receptors in cells of paraffin-processed breast cancers by IGSS. Histochemistry 87:185–187, 1987.

    Article  PubMed  CAS  Google Scholar 

  26. Wells M, Bennett J, Holgate CS: (unpublished observations).

    Google Scholar 

  27. De Waele M, De Mey J, Reynaert P, et al: Detection of cell surface antigens in cryostat sections with immunogold-silver staining. Am J Clin Pathol 85:573–578, 1986.

    PubMed  Google Scholar 

  28. Varndell IM, Polak JM, Sikri KL, et al: Visualisation of messenger RNA directing peptide synthesis by in situ hybridisation using a novel single-stranded cDNA probe. Histochemistry 81:597–601, 1984.

    Article  PubMed  CAS  Google Scholar 

  29. Lievsi P, Julien J-P, Pekka V, et al: Specific detection of neuronal cell bodies: In situ hybridisation with a biotin-labeled neurofilament cDNA probe. J Histochem Cytochem 34:923–926, 1986.

    Article  Google Scholar 

  30. Lageman A, Buchheim P: The gold-silver technique—A new immunohistochemical method for detection of cell membrane antigens. Acta Histochem 76:113–120, 1985.

    Article  PubMed  CAS  Google Scholar 

  31. Romasco F, Rosenberg J, Wybran J: An immunogold silver staining method for the light microscopic analysis of blood lymphocyte subsets with monoclonal antibodies. Am J Clin Pathol 48:307–315, 1985.

    Google Scholar 

  32. Perry PE, Thomson EJ: Immunogold labeling of metaphase cells. Cytogenet Cell Genet 41:121–125, 1986.

    Article  PubMed  CAS  Google Scholar 

  33. De Waele M, De Mey J, Renmans W, et al: An immunogold-silver staining method for detection of cell-surface antigens in light microscopy. J Histochem Cytochem 34:935–939, 1986.

    Article  PubMed  Google Scholar 

  34. Van den Pol AN: Silver-intensified gold and peroxidase as dual ultrastructural immunolabels for pre- and postsynaptic neurotransmitters. Science 228:332–335, 1985.

    Article  PubMed  Google Scholar 

  35. Birrell GB, Habliston DL, Hedburg KK, et al: Silver-enhanced gold as a cell surface marker for photoelectron microscopy. J Histochem Cytochem 34:339–345, 1986.

    Article  PubMed  CAS  Google Scholar 

  36. Holgate CS, Jackson P, Pollard K, et al: Effect of fixation on T and B lymphocyte surface membrane antigen demonstration in paraffin-processed tissue. J Pathol 149:293–300, 1986.

    Article  PubMed  CAS  Google Scholar 

  37. Dienes HP, Essling K, John HD: Application of immunoelectron microscopy for identification of T lymphocyte subsets and HLA DR antigen positive cells in tissue. J Histochem Cytochem 32:547–551, 1984.

    Article  PubMed  CAS  Google Scholar 

  38. Pollard K, Lunny D, Holgate CS, Jackson P, Bird CC: Fixation, processing and immunochemical reagent effects on preservation of T-lymphocyte surface membrane antigens in paraffin-embedded tissue. J Histochem Cytochem 35:1329–1338, 1987.

    Article  PubMed  CAS  Google Scholar 

  39. Fritz P, Hoenes J, Schenk J, et al: Colour development of immunogold-labelled antibodies for light microscopy. Histochemistry 85:209–214, 1986.

    Article  PubMed  CAS  Google Scholar 

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Authors
  1. C. S. Holgate
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  2. K. Pollard
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  3. P. Jackson
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  4. D. Lunny
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  5. P. N. Cowen
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  6. C. C. Bird
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Editor information

Editors and Affiliations

  1. University of New Mexico, New Mexico, USA

    Cecilia M. Fenoglio-Preiser M.D. (Professor of Pathology, Director of Laboratory Services) (Professor of Pathology, Director of Laboratory Services)

  2. Albuquerque Veterans Administration Medical Center, Albuquerque, New Mexico, USA

    Cecilia M. Fenoglio-Preiser M.D. (Professor of Pathology, Director of Laboratory Services) (Professor of Pathology, Director of Laboratory Services)

  3. Department of Pathology, Columbia University College of Physicians and Surgeons, Memorial Hospital, Morristown, New Jersey, USA

    Marianne Wolff M.D. (Professor of Clinical Surgical Pathology) (Professor of Clinical Surgical Pathology)

  4. National Cancer Institute, Milan, Italy

    Franco Rilke M. D. (Deputy Director, Director, Professor) (Deputy Director, Director, Professor)

  5. Division of Pathology, Postgraduate School of Anatomic Pathology, University of Pavia, Italy

    Franco Rilke M. D. (Deputy Director, Director, Professor) (Deputy Director, Director, Professor)

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© 1989 Springer-Verlag Berlin Heidelberg

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Holgate, C.S., Pollard, K., Jackson, P., Lunny, D., Cowen, P.N., Bird, C.C. (1989). New Methods of Antigen Localization and Preservation in Paraffin Processed Tissues. In: Fenoglio-Preiser, C.M., Wolff, M., Rilke, F. (eds) Progress in Surgical Pathology. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-662-12814-5_3

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  • DOI: https://doi.org/10.1007/978-3-662-12814-5_3

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-662-12816-9

  • Online ISBN: 978-3-662-12814-5

  • eBook Packages: Springer Book Archive

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