Abstract
Recent work in our laboratory involving cell-free lipid transfer from transitional endoplasmic reticulum (ER) to Golgi apparatus has shown a latent phospholipase A activity localized in the cis Golgi of rat liver (Moreau 1991). Subsequently, phosphatidylethanolamine (PE) was shown to be an important component of ER to Golgi transport vesicles. Because PE has a small hydrophilic head group and a large hydrophobic tail, it has a conical shape which is very conducive to Hexagonal II phase structure formation. The possibility that we examined was that the lysophosphatidylcholine (lysoPC), formed by the phospholipase A activity, could interact with the PE to destabilize the Hexagonal II phase and form a bilayer. However, because levels of PE decrease through the Golgi stacks, the presence of lysoPC will then destabilize the bilayer structure. To retain a stabile bilayer structure, the lysoPC must be removed. This can be accomplished in two ways: 1) degradation by a Golgi lysophospholipid phospholipase or 2) reacylation by a Golgi acyl-CoA:lysoPC acyltransferase activity. The reacylation of Golgi apparatus lysoPC via the Lands pathway (Lands 1976) was investigated.
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References
T. W. Keenan, Department of Biochemistry and Nutrition VPI Blacksburg, VA 24061
P. Moreau, CNRS 1 rue Camille-Saint-Saens 33077 Bordeaux Cedex France
Lands, W. E. M. and Crawford, C. G. (1976) Enzymes of Membrane Phospholipid Metabolism in Animals, In The Enzymes of Biological Membranes (Edited by Martouosi, A. ), Vol. 2, pp. 3–85 Plenum Press, New York.
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Morré, D. J., Cheetham, R. D., Nyquist, S. E. and Ovtracht, L. (1972) A Simplified Procedure For Isolation of Golgi Apparatus From Rat Liver, Prep. Biochem. 2, 61–69
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© 1993 Springer-Verlag Berlin Heidelberg
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Lawrence, J.B., Keenan, T.W., Moreau, P., Morré, D.J. (1993). Acyl Transfer Reactions Associated with Cis Golgi Apparatus. In: Morré, D.J., Howell, K.E., Bergeron, J.J.M. (eds) Molecular Mechanisms of Membrane Traffic. NATO ASI Series, vol 74. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-662-02928-2_12
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DOI: https://doi.org/10.1007/978-3-662-02928-2_12
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