Summary
The effect of altered thyroid hormone status on myosin heterogeneity and catecholamine enzymes was investigated in ventricles and atria of the rat heart. Hypothyroidism (0.08 % propylthiouracil in drinking water for 6 weeks) and hyper-thyroidism (daily 1 mg/kg thyroxine intraperitoneally for 2 weeks) resulted in homogeneous ventricular myosin (VM)-3 and VM-1, respectively, in rat ventricles. No such marked changes in the isoenzyme population were observed in atrial myosins using also as criterion the separation by non-dissociating pyrophosphate gel electrophoresis. Since the ventricular myosin isoenzyme population can additionally be altered by adrenergic agonists, the catecholamine enzymes phenyl-ethanolamine-N-methyltransferase (PNMT), catechol-O-methyltransferase (COMT) and monoamine oxidase (MAO) were studied in ventricles versus atria of hypothyroid and hyperthyroid rats. As in the case of myosin isoenzymes, the response of PNMT, COMT and MAO to altered circulating thyroid hormones was different when determined in ventricles compared to atria. In ventricles, PNMT, COMT, and MAO activities were significantly reduced in hyperthyroid rats compared to hypothyroid rats, whereas an opposite change was found in atria: the enzyme activities were higher for the hyperthyroid relative to the hypothyroid state. The results suggest that ventricles and atria of rat heart differ in their response towards circulating thyroid hormones not only with respect to myosin heterogeneity, but also to catecholamine enzymes. The different action of altered thyroid status on catecholamine metabolism could thus be considered a factor contributing to the expression of the above myosin isoenzyme populations.
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© 1983 Dr. Dietrich Steinkopff Verlag, GmbH & Co. KG, Darmstadt
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Bukhari, A.R., Rupp, H. (1983). Differential effect of thyroid hormones on catecholamine enzymes and myosin isoenzymes in ventricles and atria of the rat heart. In: Jacob, R., Gülch, R.W., Kissling, G. (eds) Cardiac Adaptation to Hemodynamic Overload, Training and Stress. Steinkopff. https://doi.org/10.1007/978-3-642-85326-5_7
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DOI: https://doi.org/10.1007/978-3-642-85326-5_7
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