Abstract
The second intron of the mouse Eb gene contains a well-defined recombinational hotspot. A comparison of standard laboratory MHC haplotypes suggests a region of 650 bp in the 3’ end of this intron, corresponding to the location of the breakpoint region of the recombinational hotspot, is highly conserved. This conserved segment was examined in several species and subspecies of Mus and revealed nucleotide diversity ranging from zero to 4.3%. Sequence analysis also resulted in the identification of a variable mononucleotide repeat within the proposed recombinational hotspot which shows at least eight different sequence configurations. In addition, a variable number tandem repeat (VNTR), previously identified in the 3′ end of the intron, was examined for diversity. This analysis revealed a wide variation in the number of AGGC repeats observed ranging from as few as 5 (Mus caroli) to as many as 21 (Mus musculus brevirostris). Further, a second VNTR, consisting of the tetramer TGGA was also detected immediately preceding the AGGC tandem repeat.
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Sant’Angelo, D., Heine, D., Passmore, H. (1991). Diversity and Evolution at the Eb Recombinational Hotspot in the Mouse. In: Klein, J., Klein, D. (eds) Molecular Evolution of the Major Histocompatibility Complex. NATO ASI Series, vol 59. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-84622-9_38
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DOI: https://doi.org/10.1007/978-3-642-84622-9_38
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