Comparison of Urinary Modified Nucleosides and Bases in Rats with Hepatomas and Nephroblastomas

  • I. Clark
  • J. W. MacKenzie
  • J. R. McCoy
  • W. Lin
Part of the Recent Results in Cancer Research/Fortschritte der Krebsforschung/Progrès dans les recherches sur Ie cancer book series (RECENTCANCER, volume 84)

Abstract

It has been known for more than 20 years that patients with cancer excrete greater than normal amounts of purine and pyrimidine derivatives in their urines (Adams et al. 1960; Park et al. 1962). The unmodified purine derivatives, adenine and guanine, are normally metabolized to uric acid, but in some instances they are not metabolized further and may be excreted or recycled into nucleic acids. The unmodified pyrimidine derivatives, cytosine and uracil, are usually degraded and excreted as ammonia and urea, but recycling and excretion may occur. It was not until the pioneering studies by Borek and his students on tRNA methyltransferases (Fleissner and Borek 1963) that the methylated nucleosides in the urine were shown to be derived from tRNA (Mandel et al. 1966). More recently, using more sensitive techniques, several groups of investigators have reported increased levels of modified nucleosides in urines from patients with cancer (Hogan et al. 1970; Waalkes et al. 1973; Mrochek et al. 1974; Senftleber et al. 1976; Davis et al. 1977; Gehrke et al. 1978; Speer et al. 1979; Hartwick et al. 1980). The increased levels of most modified nucleosides and bases in urine of hosts with neoplasms have been attributed to an increased rate of turnover of tRNA in cancer tissue (Borek et al. 1977). The evidence to date suggests that modified nucleosides or bases are not metabolized further and are quantitatively excreted into the urine (Weissman et al. 1962; Dlugajczyk and Eiler 1966). More than 20 modified nucleosides have been isolated from human urine (Chedda 1975).

Keywords

Posterior Silk Gland Modify Nucleoside Methylase Activity Urinary Pattern Urinary Nucleoside 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Abbreviations

ψ

pseudouridine

m1A

1-methyladenosine

m1G

1-methylguanosine

m22G

N 2 2 dimethylgua-nosine

m2G

2-methylguanosine

m1I

1-methylinosine

m3C

3-methylcytidine

m5C

5-methylcytidine

m6A

6-methyladenosine

Urn

2′-O-methyluridine

m7Gua

7-methylguanine

Ura

uracil

m1H

1-methylhypoxanthine

Thy

thymine

dT

thymidine

UV

ultraviolet light

DMSO

dimethylsulfoxide

HPLC

high-pressure liquid chromatography

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Copyright information

© Springer-Verlag Berlin • Heidelberg 1983

Authors and Affiliations

  • I. Clark
    • 1
  • J. W. MacKenzie
    • 1
  • J. R. McCoy
    • 1
  • W. Lin
    • 1
  1. 1.Departments of Surgery, Biochemistry, and PathologyCMDNJ-Rutgers Medical SchoolPiscatawayUSA

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