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PCR Amplification of DNA Recovered from the Aquatic Environment

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Nucleic Acids in the Environment

Part of the book series: Springer Lab Manuals ((SLM))

Abstract

Polymerase chain reaction (PCR; Atlas and Bej 1993; Mullis and Faloona 1987; Mullis 1990; Saiki et al. 1988; Steffan and Atlas 1988) amplification of nucleic acids from aquatic environments is an important tool to study the taxonomy, species diversity, distribution, occurrence, community structure, and seasonal variation of microorganisms. It is highly specific and analysis is rapid. In addition, water bodies including potable waters contaminated with microbial pathogens (including viruses and protozoans) are involved in the transmission of infectious diseases. Water supplies also serve as a reservoir for pathogens such as Legionella pneumophila, which causes Legionnaires’ disease when disseminated from air-conditioning cooling towers, humidifiers, hot tubs, whirlpools, and swimming pools. Routine monitoring of these pathogens by conventional culturing methods indicates the requirement for disinfection measurements if the level of detectable pathogen demonstrates the probability of a disease outbreak. The United States Environmental Protection Agency (U.S. EPA) mandates monitoring of pathogens such as Salmonella, Shigella, and Giardia and indicator organisms (coliform bacteria and Escherichia coli) in drinking water and water supplies as a measure of safety of human health.

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  1. A. K. Bej
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Editors and Affiliations

  1. Department of Environmental Biology, University of Guelph Lab. Microbial Techniques, N1G 2W1, Guelph, Ontario, Canada

    Jack T. Trevors

  2. DLO-Institute for Soil Fertility Research c/o IPO-DLO, Binnenhaven 12, P.O. Box 9060, 6700 GW, Wageningen, The Netherlands

    J. Dick van Elsas

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Bej, A.K. (1995). PCR Amplification of DNA Recovered from the Aquatic Environment. In: Trevors, J.T., van Elsas, J.D. (eds) Nucleic Acids in the Environment. Springer Lab Manuals. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-79050-8_10

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  • DOI: https://doi.org/10.1007/978-3-642-79050-8_10

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