Nucleic Acids in the Environment

1. Front Matter

   Pages i-x

   PDF

2. Introduction to Nucleic Acids in the Environment: Methods and Applications

   • J. T. Trevors, J. D. van Elsas

   Pages 1-7

3. Recovery of Bacterial Cells from Soil

   • L. R. Bakken, V. Lindahl

   Pages 9-27

4. Extraction, Purification, and Analysis of DNA from Soil Bacteria

   • V. Torsvik, F. L. Daae, J. Goksøyr

   Pages 29-48

5. Extraction and Analysis of Microbial DNA from Soil

   • A. Saano, E. Tas, S. Pippola, K. Lindström, J. D. van Elsas

   Pages 49-67

6. Extraction and Amplification of DNA from the Rhizosphere and Rhizoplane of Plants

   • K. Leung, J. T. Trevors, J. D. van Elsas

   Pages 69-87

7. Extraction of DNA from the Phyllosphere

   • M. J. Bailey

   Pages 89-109

8. Specific DNA Sequences for Detection of Soil Bacteria

   • X. Nesme, C. Picard, P. Simonet

   Pages 111-139

9. PCR Amplification of DNA from Root Nodules

   • I. L. Pepper, S. D. Pillai

   Pages 141-151

10. Extraction of DNA and RNA from Aquatic Environments

    • J. H. Paul, S. L. Pichard

    Pages 153-177

11. PCR Amplification of DNA Recovered from the Aquatic Environment

    • A. K. Bej

    Pages 179-218

12. Extraction and Amplification of 16S rRNA Genes from Deep Marine Sediments and Seawater to Assess Bacterial Community Diversity

    • P. A. Rochelle, J. A. K. Will, J. C. Fry, G. J. S. Jenkins, R. J. Parkes, C. M. Turley et al.

    Pages 219-239

13. Application of the PCR for Detection of Antibiotic Resistance Genes in Environmental Samples

    • K. Smalla, J. D. van Elsas

    Pages 241-256

14. Back Matter

    Pages 257-260

    PDF

Molecular biological techniques such as DNA/RNA extraction and purification, and especially the polymerase chain reaction, PCR, are rapidly gaining interest also in related fields, such as microbiology or environmental sciences. They offer new approaches and opportunities for the determination of microbial cells, DNA and RNA from soils, roots, rhizospheres, sediments and aquatic environments.
Detailed protocols for these applications are described in this manual.

• Boden
• Bodenbakterien
• DNA
• DNA extraction
• PCR
• Termination
• Viruses
• bacteria
• biomass
• genes
• microorganism
• microorganisms
• polymerase chain reaction
• rhizosphere
• ribosomal RNA

"The protocols that are presented are well defined in a step-by step manner. This should allow their easy implementation in a laboratory. An attractive feature ... is the inclusion of a series of tables with suggested primers for analysis of specific genes." Quarterly Review of Biology

• Department of Environmental Biology, University of Guelph Lab. Microbial Techniques, Guelph, Canada

  Jack T. Trevors

• DLO-Institute for Soil Fertility Research c/o IPO-DLO, Wageningen, The Netherlands

  J. Dick Elsas

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