Abstract
Several limitations in transport properties of the outer mitochondrial membrane were found in intact mitochondria of porin-deficient mutants of S. cerevisiae, which grows as efficient as the isogenic wild strain. Restrictions concerned the effectiveness of ADP and CATR in triggering and terminating the phosphorylating state and of the external NADH in supporting the electron flow, with all three types of molecules apparently competing for the entry. These restrictions disappeared in mitoplasts obtained from mutant mitochondria. The existence of another pore is postulated. The novel pore seems to be controlled by cations which might act either directly on the pore or indirectly by screening the negative charge of the transported species. The novel pore is similarly sensitive to the synthetic polyanion as is VDAC; in the absence of VDAC the outer mitochondrial membrane is easily damaged by this inhibitor.
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© 1994 Springer-Verlag Berlin Heidelberg
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Michejda, J., Kmita, H., Stobienia, O., Budzińska, M., Lauquin, G.JM. (1994). Restrictions of Metabolite Permeation Through the Outer Mitochondrial Membrane of Porin-Deficient Yeast Mutant. In: Forte, M., Colombini, M. (eds) Molecular Biology of Mitochondrial Transport Systems. NATO ASI Series, vol 83. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-78936-6_24
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DOI: https://doi.org/10.1007/978-3-642-78936-6_24
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