Transformation in Duboisia spp.
The genus Duboisia consists of three species, Duboisia leichhardtii F. Muell, D. myoporoides R. Br., and D. hopwoodii F. Muell. Duboisia species are woody plants and the former two species produce the largest amounts of tropane alkaloids among the Solanaceae, which includes the other alkaloid-producing plants Atropa, Datura, Hyoscyamus and Scopolia. The major alkaloids present in D. leichhardtii and D. myoporoides are scopolamine and hyoscyamine, important compounds that have been used as spasmolytics and anesthetics. The third species, D. hopwoodii, produces mainly nicotine and nornicotine, both of which are nicotine alkaloids normally found in tobacco plants. Both tropane and nicotine alkaloids are synthesized mainly in the roots of these three species.
KeywordsHairy Root Somatic Hybrid Hairy Root Culture Alkaloid Content Agrobacterium Rhizogenes
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- Armitage P, Waiden R, Draper J (1988) Vectors for the transformation of plant cells using Agrobacterium. In: Draper J, Scott R, Armitage P, Waiden R (eds) Plant genetic transformation and gene expression, a laboratory manual. Blackwell, Oxford, pp 1–67.Google Scholar
- Birot AM, Bouchez D, Casse-Delbart F, Durant-Tardif M, Jouanin L, Pautot V, Robaglia C, Tepfer D, Tepfer M, Tourneur J, Vilaine F (1987) Studies and uses of the Ri plasmids of Agrobacterium rhizogenes. Plant Physiol Biochem 25:323–335.Google Scholar
- Deno H, Yamagata H, Emoto T, Yoshioka T, Yamada Y, Fujita Y (1987) Scopolamine production by root cultures of Duboisia myoporoides. II. Establishment of a hairy root culture by infection with Agrobacterium rhizogenes. J Plant Physiol 131:315–323.Google Scholar
- Dougall DK (1987) Cell cloning and the selection of high yielding strains. In: Constabel F, Vasil IK (eds) Cell culture and somatic cell genetics of plants vol 4. Academic Press, San Diego, pp 117–124.Google Scholar
- Endo T, Komiya T, Masumitsu Y, Morikawa M, Yamada Y (1987) An intergeneric hybrid cell line of Duboisia hopwoodii and Nicotiana tabacum by protoplast fusion. J Plant Physiol 129:453–459.Google Scholar
- Strauss A (1989) Hyoscyamus spp: In vitro culture and the production of tropane alkaloids. In: Bajaj YPS (ed) Biotechnology in agriculture and forestry vol 7. Medicinal and aromatic plants II Springer, Berling Heidelberg New York, pp 286–314.Google Scholar