Skip to main content
SpringerLink
Log in

Transformation in Datura Species

  • Chapter
Plant Protoplasts and Genetic Engineering III

Part of the book series: Biotechnology in Agriculture and Forestry ((AGRICULTURE,volume 22))

Abstract

One of the main reasons why there are so few commercial processes which utilize plant cell cultures on a large scale is the cost effectiveness of the maintenance and processing of sterile cultures versus yield. Poor secondary metabolite biosynthesis and culture instability have prevented commercialization of many plant culture systems. Secondary products are frequently produced in cell cultures in lower yield than in the parent plant; however, there are more than 30 examples where the yield of secondary compounds from plant cell cultures is equal to or in excess of that produced by the parent plant (Phillipson 1990). Providing selection for high-yielding cells can be made and stable cultures produced, commercial exploitation may be considered, providing that the market cost of the compound produced allows the process to be cost-effective.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Log in via an institution
Chapter
USD 29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 84.99
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 109.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  • Al-Yahya M, Evans WC (dy1975) Alkaloids of the F1 hybrid of Datura stramonium x D. discolor. J Pharm Pharmacol 27:87p (Suppl).

    Google Scholar 

  • Bristol ML, Evans WC, Lampard JF (1969) The alkaloids of the genus Datura, section Brugmansia. Part VI. Tree Datura drugs (Datura Candida cvs.) of the Colombian Sibundoy. J Nat Prod (Lloydia) 32:123–130.

    CAS  Google Scholar 

  • Christen P, Roberts MF, Phillipson JD, Evans WC (1989) High-yield production of tropane alkaloids by hairy-root cultures of a Datura Candida hybrid. Plant Cell Rep 8:75–77.

    Article  CAS  Google Scholar 

  • Christen P, Roberts MF, Phillipson JD, Evans WC (1990) Alkaloids of hairy root cultures of a Datura Candida hybrid. Plant Cell Rep 9:101–104.

    Article  CAS  Google Scholar 

  • Clare BG (1990) Agrobacterium in plant disease, biological disease control and plant genetic engineering. Sci Progr London 74:1–13.

    PubMed  CAS  Google Scholar 

  • El-Dabbas SW, Evans WC (1982) Alkaloids of the genus Datura, section Brugmansia. X. Alkaloid content of Datura hybrids. Planta Med 44:184–185.

    Article  PubMed  CAS  Google Scholar 

  • El-Imam YMA, Evans WC (1990) Alkaloids of a Datura Candida cultivar, D. aurea and various hybrids. Fitoterapia 61:148–152.

    CAS  Google Scholar 

  • Ellis JG, Ryder MH, Tate ME (1984) Agrobacterium tumefaciens TR-DNA encodes a pathway for agropine biosynthesis. Mol Gen Genet 195:466–473.

    Article  CAS  Google Scholar 

  • Evans WC (1979) Tropane alkaloids of the Solanaceae. In: Hawkes JG, Lester RN, Skelding AD (eds) The biology and taxonomy of the Solanaceae. Academic Press, New York, pp 241–254.

    Google Scholar 

  • Evans WC (1990) Datura, a commercial source of hyoscine. Pharm J 244:651–653.

    Google Scholar 

  • Flores HE, Hoy MW, Pickard JJ (1987) Secondary metabolites from root cultures. Trends Biotechnol 5:64–69.

    Article  CAS  Google Scholar 

  • Furze JM, Rhodes MJC, Parr AJ, Robins RJ, Withehead IM, Threlfall DR (1991) Abiotic factors elicit sesquiterpenoid phytoalexin production but not alkaloid production in transformed root cultures of Datura stramonium. Plant Cell Rep. 10:111–114.

    Article  CAS  Google Scholar 

  • Gamborg OL, Miller RA, Ojima K (1968) Nutrient requirements of suspension cultures of soybean root cells. Exp Cell Res 50:151–158.

    Article  PubMed  CAS  Google Scholar 

  • Griffin WJ (1976) Agronomic evaluation of Datura Candida — a new source of hyoscine. Econ Bot 30:361–369.

    Article  CAS  Google Scholar 

  • Griffin WJ (1992) Alkaloids of a Datura Candida cultivar. Phytochemistry 31:367–368.

    Article  CAS  Google Scholar 

  • Hashimoto T, Yamada Y (1986) Hyoscyamine 6β-hydroxylase, a 2-oxoglutarate-dependant dioxygenase, in alkaloid producing root cultures. Plant Physiol 81:619–625.

    Article  PubMed  CAS  Google Scholar 

  • Hashimoto T, Kohno J, Yamada Y (1989) 6β-Hydroxyhyoscyamine epoxidase from cultured roots of Hyoscyamus niger. Phytochemistry 28:1077–1082.

    Article  CAS  Google Scholar 

  • Hashimoto T, Hayashi A, Amano Y, Fohno J, Iwanari H, Usuda S, Yamada Y (1991) Hyoscyamine 6β-hydroxylase, an enzyme involved in tropane alkaloid biosynthesis, is localized at the pericycle of the root. J Biol Chem 266:4648–4653.

    PubMed  CAS  Google Scholar 

  • Hay CA, Anderson LA, Roberts MF, Phillipson JD (1988) Alkaloid production by plant cell cultures. In: Mizrahi A (ed) Biotechnology in agriculture, advances in biotechnological processes, vol 9. Alan R. Riss, New York, pp 97–140.

    Google Scholar 

  • Hilton MG, Rhodes MJC (1990) Growth and hyoscyamine production of hairy root cultures of Datura stramonium in a modified stirred tank reactor. Appl Microbiol Biotechnol 33:132–138.

    Article  PubMed  CAS  Google Scholar 

  • Hooykaas PJJ (1989) Transformation of plant cells via Agrobacterium. Plant Mol Biol 13:327–336.

    Article  PubMed  CAS  Google Scholar 

  • Jaziri M, Legros M, Homes J, Vanhaelen M (1988) Tropane alkaloids production by hairy root cultures of Datura stramonium and Hyoscyamus niger. Phytochemistry 27:419–420.

    Article  CAS  Google Scholar 

  • Jung G, Tepfer D (1987) Use of genetic transformation by the Ri T-DNA of Agrobacterium rhizogenes to stimulate biomass and tropane alkaloid production in Atropa belladonna and Calystegia sepium roots grown in vitro. Plant Sci 50:145–151.

    Article  CAS  Google Scholar 

  • Kamada H, Okamura N, Satake M, Harada H, Shimomura K (1986) Alkaloid production by hairy root cultures in Atropa belladonna. Plant Cell Rep 5:239–242.

    Article  CAS  Google Scholar 

  • Knopp E, Strauss A, Wehrli W (1988) Root induction on several Solanaceae species by Agrobacterium rhizogenes and the determination of root tropane alkaloid content. Plant Cell Rep 7:590–593.

    Article  CAS  Google Scholar 

  • Leete E (1990) Recent developments in the biosynthesis of tropane alkaloids. Planta Med 56:339–352.

    Article  PubMed  CAS  Google Scholar 

  • Lounasmaa M (1988) The tropane alkaloids. In: Brossi A (ed) The alkaloids, vol 33. Academic Press, New York, pp 1–81.

    Google Scholar 

  • Mano Y, Nabeshima S, Matsui C, Ohkawa H (1986) Production of tropane alkaloids by hairy root cultures of Scopolia japonica. Agric Biol Chem 50:2715–2722.

    Article  CAS  Google Scholar 

  • Mano Y, Ohkawa H, Yamada Y (1989) Production of tropane alkaloids by hairy root cultures of Duboisia leichhardtii transformed by Agrobacterium rhizogenes. Plant Sci 59:191–201.

    Article  CAS  Google Scholar 

  • Munier J (1988) Establishment of new axenic hairy root lines by inoculation with Agrobacterium rhizogenes. Plant Cell Rep 7:9–12.

    Article  Google Scholar 

  • Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15:473–497.

    Article  CAS  Google Scholar 

  • Nabeshima S, Mano Y, Ohkawa H (1986) Production of tropane alkaloids by hairy root cultures of Scopolia japonica. Symbiosis 2:11–18.

    CAS  Google Scholar 

  • Ohkawa H, Kamada H, Sudo H, Harada H (1989) Effects of gibberellic acid on hairy root growth in Datura innoxia. J Plant Physiol 134:633–636.

    CAS  Google Scholar 

  • Parr AJ, Hamill JD, Payne J, Rhodes MJC, Robins RJ, Walton NJ (1988) Exploiting variation in tropane alkaloid production by solanaceous plants. In: Robins RJ, Rhodes MJC (eds) Manipulating secondary metabolism in culture. Cambridge University Press, Cambridge, pp 101–107.

    Google Scholar 

  • Parr AJ, Payne J, Eagles J, Chapman BT, Robins RJ, Rhodes MJC (1990) Variation in tropane alkaloid accumulation within the Solanaceae and strategies for its exploitation. Phytochemistry 29:2545–2550.

    Article  CAS  Google Scholar 

  • Payne J, Hamill JD, Robins RJ, Rhodes MJC (1987) Production of hyoscyamine by hairy root cultures of Datura stramonium. Planta Med 53:474–478.

    Article  PubMed  CAS  Google Scholar 

  • Petit A, David C, Dahl GA, Ellis JG, Guyon P, Casse-Delbart F, Tempé J (1983) Further extension of the opine concept: plasmids in Agrobacterium rhizogenes cooperate for opine degradation. Mol Gen Genet 190:204–214.

    Article  CAS  Google Scholar 

  • Petri G, Bajaj YPS (1989) Datura spp.: In vitro regeneration and the production of tropanes. In: Bajaj YPS (ed) Biotechnology in agriculture and forestry, vol 7. Medicinal and aromatic plants II. Springer, Berlin Heidelberg New York, pp 135–161.

    Google Scholar 

  • Phillipson JD (1990) Plants as sources of valuable products. In: Charlwood BV, Rhodes MJC (eds) Secondary products from plant tissue culture. Clarendon Press, Oxford, pp 1–21.

    Google Scholar 

  • Rhodes MJC, Robins RJ, Hamill JD, Parr AJ, Walton NJ (1987) Secondary product formation using Agrobacterium rhizogenes-transformed hairy root culture. IAPTC Newslett 53:2–15.

    Google Scholar 

  • Robins RJ, Parr AJ, Payne J, Walton NJ, Rhodes MJC (1990) Factors regulating tropane-alkaloid production in a transformed root culture of a Datura Candida x D. aurea hybrid. Planta 181:414–422.

    Article  CAS  Google Scholar 

  • Robins RJ, Parr AJ, Bent EG, Rhodes MJC (1991a) Studies on the biosynthesis of tropane alkaloids in Datura stramonium L. transformed root cultures. 1. The kinetics of alkaloid production and the influence of feeding intermediate metabolites. Planta 183:185–195.

    Article  CAS  Google Scholar 

  • Robins RJ, Parr AJ, Walton NJ (1991b) Studies on the biosynthesis of tropane alkaloids in Datura stramonium L. transformed root cultures. 2. On the relative contributions of L-arginine and L-ornithine to the formation of the tropane ring. Planta 183:196–201.

    Article  CAS  Google Scholar 

  • Robins RJ, Bent EG, Rhodes MJC (1991c) Studies on the biosynthesis of tropane alkaloids by Datura stramonium L. transformed root cultures. Planta 185:385–390.

    Article  CAS  Google Scholar 

  • Robins RJ, Bachmann P, Robinson T, Rhodes MJC, Yamada Y (1991d) The formation of 3α-and 3α-acetoxytropanes by Datura stramonium transformed root cultures involves two acetyl-CoA-dependent acyltransferases. FEBS Lett. 292:293–297.

    Article  PubMed  CAS  Google Scholar 

  • Romeike A (1962) Weitere Versuche zur Züchtung einer scopolaminreichen Datura-Hybnde. Kulturpflanze 10:140–148.

    Article  Google Scholar 

  • Safford WE (1921) Synopsis of the genus Datura. J Wash Acad Sci 11:173–189.

    Google Scholar 

  • Scragg AH (1990) Fermentation systems for plant cells. In: Charlwood BV, Rhodes MJC (eds) Secondary products from plant tissue culture. Clarendon Press, Oxford, pp 243–263.

    Google Scholar 

  • Spencer PA, Towers GHN (1988) Specificity of signal compounds detected by Agrobacterium tumefaciens. Phytochemistry 27:2781–2785.

    Article  CAS  Google Scholar 

  • Shimomura K, Sauerwein M, Ishimaru K (1991) Tropane alkaloids in the adventitious and hairy root cultures of solanaceous plants. Phytochemistry 30:2275–2278.

    Article  CAS  Google Scholar 

  • Walton NJ, Robins RJ, Peerless ACJ (1990) Enzymes of N-methylputrescine biosynthesis in relation to hyoscyamine formation in transformed root cultures of Datura stramonium and Atropa belladonna. Planta 182:136–141.

    Article  CAS  Google Scholar 

  • Wettstein R (1895) Solanaceae. In: Engler A, Prantl K (eds) Die natürlichen Pflanzenfamilien. Englemann, Leipzig, IV (3b) pp 4–38.

    Google Scholar 

  • Witte L, Müller K, Arfmann H-A (1987) Investigation of the alkaloid pattern of Datura innoxia plants by capillary gas-liquid chromatography-mass spectrometry. Planta Med 53:192–197.

    Article  PubMed  CAS  Google Scholar 

  • Yamada Y, Hashimoto T, Endo T, Yukimune Y, Kohno J, Hamaguchi N, Präger B (1990) Biochemistry of alkaloid production in vitro. In: Charlwood BV, Rhodes MJC (eds) Secondary products from plant tissue culture. Clarendon Press, Oxford, pp 227–242.

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Dépt. de Pharmacognosie, Ecole de Pharmacie, Université de Genève, 30, Quai E.-Ansermet, 1211, Genève 4, Switzerland

    P. Christen

  2. Dept. of Pharmacognosy, The School of Pharmacy, University of London, London, WC1N 1AX, UK

    M. F. Roberts

Authors
  1. P. Christen
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. M. F. Roberts
    View author publications

    You can also search for this author in PubMed Google Scholar

Editor information

Editors and Affiliations

  1. A-137 New Friends Colony, New Delhi, 110065, India

    Y. P. S. Bajaj

Rights and permissions

Reprints and permissions

Copyright information

© 1993 Springer-Verlag Berlin Heidelberg

About this chapter

Cite this chapter

Christen, P., Roberts, M.F. (1993). Transformation in Datura Species. In: Bajaj, Y.P.S. (eds) Plant Protoplasts and Genetic Engineering III. Biotechnology in Agriculture and Forestry, vol 22. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-78006-6_14

Download citation

  • DOI: https://doi.org/10.1007/978-3-642-78006-6_14

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-78008-0

  • Online ISBN: 978-3-642-78006-6

  • eBook Packages: Springer Book Archive

Publish with us

Policies and ethics

Search

Navigation