Abstract
Adsorption is the simplest and the oldest method of immobilizing an enzyme onto a water-insoluble carrier. It has already been mentioned (p. 18) that as early as 1916 Nelson and Griffin observed that invertase adsorbed on active charcoal retained its sucrose-splitting activity. Since this discovery, the adsorption method has been applied for innumerable enzymes and whole cells. That whole cells can be made to adhere to suitable solid bodies has been known for even longer than the adsorption of single enzymes: in the nineteenth century (see Sect. 1.6) bacteria attached to wood shavings were already used in the production of vinegar.
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Literature
BeddoMs C G, Gil M H, Guthrie J T (1986) Immobilization of BSA, enzymes and cells of Bacillus stearothermophilus onto cellulose, polygalacturonic acid and starch based craft copolymers containing maleic anhydride. Biotechnol Bioeng 28: 51–57
Cabral J M S, Novais J M, Kennedy J F (1986) Immobilization studies of whole microbial cells on transition metal activated inorganic supports. Appl Microbiol Biotechnol 23: 157–162
Hulst A C, Tramper J, Riet K van’t, Westerbeek J M M (1985) A new technique for the production of immobilized biocatalyst in large qantities. Biotechnol Bioeng 27: 870–876
Klein J, Wagner F (1983) Methods for the immobilization of microbial cells. In: Chibata I, Wingard L B (eds) Applied biochemistry and bioengineering, vol 4, Academic Press, New York, pp 11–51
Ito H, Shimizu A, Ichikizaki I (1986) Preparation and properties of stable water- insoluble derivatives of glutathione s-aryltransferase. Biotechnol Bioeng 28: 97100
Kucera J (1986) The polymeric p- and o-quinones as the reactive supports for enzymes immobilization. Biotechnol Bioeng 28: 110–111
Kumakura M, Kaetsu I, Nisizawa K (1984) Cellulase production from immobilized growing cell composites prepared by radiation polymerization. Biotechnol Bioeng 26: 17–21
Marty J-L (1985) Application of response surface methodology to optimization of glutaraldehyde activation of a support for enzyme immobilization. Appl Microbiol Biotechnol 22: 88–91
Mattiasson B (1982) Immobilization methods. In: Mattiasson B (ed) Immobilized cells and organelles, vol 1. CRC Press, Boca Raton, pp 3–25
Mayer L D, Bally M B, Hope M J, Cullis P R (1986) Techniques for encapsulating bioactive agents into liposomes. Chem Phys Lipids 40: 333–345
Okita M B, Bonham D B, Gainer J L (1985) Covalent coupling of microorganisms to a cellulosic support. Biotechnol Bioeng 27: 632–637
Papisov M I, Naksimenko A V, Torchilin V P (1985) Optimization of reaction conditions during enzyme immobilization on soluble carboxyl-containing carriers. Enz Microb Technol 7: 11–16
Rouxhet P G, Haecht J L van, Didelez J, Gerard P, Briquet M (1981) Immobilization of yeast cells by entrapment and adhesion using silicious materials. Enzyme Microbial Technol 3: 49–54
Sikyta B (1986) Methods of cell immobilization. Microbiological Sci 3: 16–17
Tanaka H, Kurosawa H, Kokufuta E, Veliky I A (1984) Preparation of immobilized glucoamylase using Ca-alginate gel coated with partially quaternized poly(ethy- leneimine). Biotechnol Bioeng 26: 1393–1394
Tsuge H, Okada T (1984) Immobilization of yeast pyridoxaminephosphate oxidase to halogenoacetyl polysaccharides. Biotechnol Bioeng 26: 412–418
Vorlop K D, Klein J (1983) New developments in the field of cell immobilization - formation of biocatalysts by ionotropic gelation. In: Lafferty R M (ed) Enzyme technology. Springer, Berlin Heidelberg New York, pp 219–235
Wang H Y, Lee S S, Takach Y, Cawthon L (1982) Maximizing microbial cell loading in immobilized cell systems. Biotechnol Bioeng Symp 12: 139–146
klongkhalaung C, Kashiwagi Y, Magae Y, Ohta T, Sasaki T (1985) Cellulase immobilized on a soluble polymer. Appl Microbiol Biotechnol 21: 37–41
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© 1988 Springer-Verlag Berlin Heidelberg
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Hartmeier, W. (1988). Methods of Immobilization. In: Immobilized Biocatalysts. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-73364-2_2
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DOI: https://doi.org/10.1007/978-3-642-73364-2_2
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