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Cellulase immobilized on a soluble polymer

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Summary

Aspergillus niger cellulase was imobilized on cyanogen bromide activated dextran of varying molecular weights. The effect of different concentrations of cyanogen bromide used for the activation process was also studied. About 50% conjugation and 70% retention activity was achieved in the immobilized cellulase. The pH activity of immobilized enzyme was unchanged, but exhibited more stable activity at acidic pH than the free enzyme. Higher resistance to heat inactivation was also observed.

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References

  • Axen R, Porath J, Ernback S (1967) Chemical coupling of peptides and proteins to polysaccharides by means of cyanogen halides. Nature 214:1302–1304

    Google Scholar 

  • Hough L (1962) Methods in carbohydrate chemistry I. Academic Press, New York, London

    Google Scholar 

  • Karube I, Tanaka S, Shirai T Suzuki S (1977) Hydrolysis of cellulose in a cellulase-bead fluidized bed reactor. Biotechnol Bioeng XIX:1183–1191

    Google Scholar 

  • Kawamura Y, Nakanishi K, Matsuno R, Kamikubo T (1981) Stability of immobilized α-chymotrypsin. Biotechnol Bioeng XXIII:1219–1236

    Google Scholar 

  • Kashiwagi Y, Magae Y, Sasaki T (1984) Preparation and some properties of immobilized cellulase on cyanogen bromide activated sepharose. J Jpn Soc Food Sci Technol 31:86–91

    Google Scholar 

  • Kuo JY, Goldstein J (1983) α-D-galactosidase immobilized on a soluble polymer. Enzyme Microbiol Technol 5:285–290

    Google Scholar 

  • Lowry OH, Rosenbrough NJ, Farr AL, Randall RJ (1951) Protein measurement with the folin phenol reagent. J Biol Chem 193:265–275

    Google Scholar 

  • Marshall JJ, Humphreys JD (1977) Synthesis of a soluble catalase — dextran conjugate. Biotechnol Bioeng XIX:1739–1760

    Google Scholar 

  • Marshall JJ, Rabinowitz ML (1975) Enzyme stabilization by covalent attachment of carbohydrate. Arch Biophys Biochem 167:777–779

    Google Scholar 

  • Marshall JJ, Rabinowitz ML (1976) Preparation and characterization of a dextran-trypsin conjugate. J Biol Chem 251:1081–1087

    Google Scholar 

  • Somogyi M (1952) Notes of sugar determination. J Biol Chem 195:19–23

    Google Scholar 

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Author notes

  1. Chakamas Wongkhalaung

    Present address: Institute of Food Research and Product Development, Kasetsart University, P.O. Box 4-170, 10400, Bankok, Thailand

Authors and Affiliations

  1. National Food Research Institute, P.O. Box 11, 305, Tsukuba Science City, Ibaraki, Japan

    Chakamas Wongkhalaung, Yutaka Kashiwagi, Yumi Magae, Teruo Ohta & Takashi Sasaki

Authors
  1. Chakamas Wongkhalaung
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  2. Yutaka Kashiwagi
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  3. Yumi Magae
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  4. Teruo Ohta
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  5. Takashi Sasaki
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Wongkhalaung, C., Kashiwagi, Y., Magae, Y. et al. Cellulase immobilized on a soluble polymer. Appl Microbiol Biotechnol 21, 37–41 (1985). https://doi.org/10.1007/BF00252359

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  • DOI: https://doi.org/10.1007/BF00252359

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