Abstract
The enzyme pullulanase (EC 3.2.1.41) is produced by various genera of Gram-positive and Gram-negative bacteria. This enzyme is defined by its ability to hydrolyze α(1–6) bonds of α(1–4), α(1–6) glucans. Pullulanases of approximately 140 kd (Eisele et al. 1972) are produced and secreted by strains of the Gram-negative bacterium Klebsiella aerogenes. The amounts synthesized and proportions secreted are strain-dependent. In this report we summarize data obtained from the analysis of Klebsiella aerogenes strain K21. This strain secretes 50% of pullulanase made into the culture medium. The remaining 50% is located at the cell surface.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
Braun V (1975) Covalent lipoprotein from the outer membrane of E. coli. Biochim Biophys Acta 415: 335–377
Chapon C, Raibaud O (1985) Structure of two divergent promoters located in front of the gene encoding pullulanase in Klebsiella pneumoniae and positively regulated by the malT· product. J Bacteriol 164: 639–645
Eisele B, Rasched IR, Wallenfels K (1972) Molecular characterisation of pullulanase from Aerobacter aerogenes. Eur J Biochem 26: 62–67
Ichihara S, Hussain M, Mizushima S (1981) Characterisation of new membrane lipoproteins and their precursors in E. coli. J Biol Chem 256: 3125–3129
Kadonaga JT, Gautier AE, Straus DR, Charles AD, Edge MD, Knowles JR (1984) The role of the βlactamase signal sequence in the secretion of proteins by E. coli. J Biol Chem 259: 2149–2154
Katsuragi N, Takizawa N, Murooka Y (1987) Entire nucleotide sequence of the pullulanase gene of Klebsiella aerogenes W70. J Bacteriol 169: 2301–2306
Kuhn I, Stephenson F, Boyer HW, Greene PJ (1986) Positive-selection vectors utilizing lethality of EcoR1 endonuclease. Gene 44: 253–263
Michaelis S, Chapon C, D’Enfert C, Pugsley AP, Schwartz M (1985) Characterisation and expression of the structural gene for pullulanase, a maltose-inducible secreted protein of Klebsiella pneumoniae. J Bacteriol 164: 633–638
Morgan FJ, Adams KR, Priest FG (1979) A cultural method for the detection of pullulan-degrading enzymes in bacteria and its application to the genus Bacillus. J Appl Bacteriol 46: 291–294
Nielsen JBK, Lampen JO (1982) Glyceride-cysteine lipoproteins and secretion by gram-positive bacteria. J Bacteriol 152: 315–322
Nilsson B, Uhlen M, Josephson S, Gatenbeck S, Philipson L (1983) An improved positive-selection plasmid vector constructed by oligonucleotide mediated mutagenesis. Nucleic Acids Res 11: 8019–8030
Ohba R, Ueda S (1982) An inductive effector in the production of extracellular pullulanase by Aerobacter aerogenes. Agric Biol Chem 46: 2425–2431
Pugsley AP, Chapon C, Schwartz M (1986) Extracellular pullulanase of Klebsiella pneumoniae is a lipoprotein. J Bacteriol 166: 1083–1088.
Salmond GPC, Hinton JCD, Gill DR, Perombelon MCM (1986) Transposon mutagenesis of Erwinia using phage λ vectors. Mol Gen Genet 203: 524–528
Takizawa N, Murooka Y (1985) Cloning of the pullulanase gene and overproduction of pullulanase in E. coli and Klebsiella aerogenes. Appl Environ Microbiol 49: 294–298
Way JC, Davis MA, Morisato D, Roberts DE, Kleckner N (1984) New Tn1O derivatives for transposon mutagenesis and for construction of lacZ operon fusions by transposition. Gene 32: 369–379
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1988 Springer-Verlag Berlin Heidelberg
About this paper
Cite this paper
Kornacker, M.G., Boyd, A., Plastow, G.S. (1988). A Genetic Analysis of Pullulanase Export from Klebsiella Aerogenes . In: Op den Kamp, J.A.F. (eds) Membrane Biogenesis. NATO ASI Series, vol 16. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-73184-6_28
Download citation
DOI: https://doi.org/10.1007/978-3-642-73184-6_28
Publisher Name: Springer, Berlin, Heidelberg
Print ISBN: 978-3-642-73186-0
Online ISBN: 978-3-642-73184-6
eBook Packages: Springer Book Archive