Abstract
This report summarizes the fourth collaborative effort in conjunction with the International Histocompatibility Workshop to use a common scheme to type for HLA-D determinants with homozygous typing cells (HTC) in the mixed lymphocyte culture (MLC) test. This method, now more than ten years old, was originally proposed by us [1] and subsequently incorporated into the Sixth, Seventh, and Eighth International Histocompatibility Workshops [2, 3, 4]. The results of these three Workshops can be summarized as follows:
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1.
At least 12 distinct HLA-D determinants (Dw1 through w12) can be recognized in the population.
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2.
Some of these determinants are heterogeneous or cross-reactive.
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3.
There is an HLA-D “blank” gene frequency of approximately 30% as evidence for additional, still unrecognized determinants.
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4.
The HLA-D determinants are in linkage disequilibrium, especially with HLA-DR and HLA-B alleles.
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5.
As for the serologically defined HLA antigens, the gene frequencies of the individual HLA-D determinants vary between different ethnic groups.
This work was supported by the Deutsche Forschungsgemeinschaft (AL-92/13–1) and by SFB102 TPB4
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References
Mempel W, Grosse-Wilde H, Albert E, Thierfelder S (1973) Atypical reactions in HL-A-typed related and unrelated pairs. Transplant Proc 5: 401–408
Thorsby E, Piazza A (1975) Joint report from the Sixth In-ternational Histocompatibility Workshop Conference. II. Typing for HLA-D (LD-1 or MLC) determinants. In: Kissmeyer-Nielsen F (ed) Histocompatibility testing 1975. Munksgaard, Copenhagen, pp 414–458
Festenstein H, Oliver RTD, Sachs JA, McMichael AJ, Bodmer WF (1978) Overall analysis of the HTC data based on clusters, patterns and antigen reports. In: Bodmer WF, Batchelor JR, Bodmer JG, Festenstein H, Morris PJ (eds) Histocompatibility testing 1977. Munksgaard, Copenhagen, pp 115–141
Dupont B, Braun DW Jr, Yunis EJ, Carpenter CB (1980) Joint report: HLA-D by cellular typing. In: Terasaki PI (ed) Histocompatibility testing 1980. UCLA Tissue Typing Laboratory, Los Angeles, pp 229–267
Mickelson EM, Nisperos B, Layrisse Z, Se Jong Kim, Donnall Thomas E, Hansen JA (1983) Analysis of the HLA-DRw8 haplotype: recognition by HTC typing of three distinct antigen complexes in Caucausians, native Americans and Orientals. Immunogenetics 17: 399–410
Ruppelt W, Grosse-Wilde H, Netzel B (1976) Computer program for the evaluation and interpretation of MLC data. Z Immun Forsch 152: 293–297
Sasazuki T, Kaneoka H, Tsuji K, Nose Y, Kashiwagi N, Kaneko T (1980) Joint report: HLA-D in Japan. In: Terasaki PI (ed) Histocompatibility testing 1980. UCLA Tissue Typing Laboratory, Los Angeles, pp 274–276
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© 1984 Springer-Verlag Berlin Heidelberg
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Grosse-Wilde, H., Doxiadis, I., Brandt, H. (1984). Definition of HLA-D with HTC. In: Albert, E.D., et al. Histocompatibility Testing 1984. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-69770-8_97
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DOI: https://doi.org/10.1007/978-3-642-69770-8_97
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