Abstract
We have used seven HLA-D homozygous typing cells (HTC) in a comparative study of the DRw8 antigen complex in three racial groups. Three distinct HLA-D specificities were recognized, each associated with HLA-DRw8. Four of the HTC defined a DRw8-associated HLA-D specificity designated 8.1, one defined a specificity designated 8.2, and two defined a specificity designated 8.3. Each of the three spec cities showed an association with a distinct racial group: Dw“8.1” in Caucasians, Dw“8.2” in Pacific Northwest Indians, and Dw“8.3” in Orientals. An informative primed lymphocyte (PLT) cell generated against a Dw“8.1” haplotype was able to distinguish 8.1 from 8.2 and 8.3. Using selected anti-DRw8 sera, a serologic distinction between 8.1 and 8.3 could also be made. It was thus possible, by using both cellular and serologic techniques in a comparative population study, to recognize at least three HLA-D-defined splits of the DRw8 haplotype.
Similar content being viewed by others
References
Carroll, P. G., DeWolf, W. C., Mehta, C. R., Rohan, J. E., and Yunis, E. J.: Centroid cluster analysis of the primed lymphocyte test. Transplant. Proc. 11: 1809–1812, 1979
Danilovs, J. A., Ayoub, G., and Terasaki, P. I.: Joint Report: B lymphocyte isolation by thrombin-nylon wool. In P.I. Terasaki (ed.): Histocompatibility Testing 1980, pp. 287–288, UCLA Press, Los Angeles, 1980
Dupont, B., Braun, D. W., Jr., Yunis, E. J., and Carpenter, C. B.: HLA-D by cellular typing. In P. I. Terasaki (ed.): Histocompatibility Testing 1980, pp. 229–267, UCLA Press, Los Angeles 1980
Festenstein, H. and Oliver, R. T. D.: Cellular typing. In W. F. Bodmer, J. R., Batchelor, J. G. Bodmer, H. Festenstein, and P.J. Morris (eds.): Histocompatibility Testing 1977, pp. 85–156, Munksgaard, Copenhagen, 1978
Layrisse, Z., Heinen, H. D., Simonney, N., Stoikow, Z., Balbas, O., Leets, I., and Garcia, E.: HLA-D typing with homozygous cells identified in an American indigenous isolate. II. Family studies and D/DR relationship. Tissue Antigens 20: 86–99, 1982
Mickelson, E. M., Nisperos, B., and Hansen, J. A.: Two homozygous typing cells defining a subgroup of HLA-Dw6. Hum. Immunol. 1: 363–368, 1980
Mickelson, E. M., Nisperos, B., Thomas, E. D., and Hansen, J. A.: Definition of LD“g4 × 7”: A unique HLA-D specificity defined by two homozygous typing cells. Hum. Immunol. 4: 79–86, 1982
NIAID Manual of Tissue Typing Techniques, 1979–1980, pp. 39–41, NIH Publication No. 80-545, 1980
Reinsmoen, N. L. and Bach, F. H.: Five HLA-D clusters associated with HLA-DR4. Hum. Immunol. 4: 249–258, 1982
Reinsmoen, N. L., Sasazuki, T., Kaneoka, H., Ohta, N., Noreen, H. J., Greenberg, L. J., and Kersey, J. H.: Two distinct HLA-D specificities (DHO and Dw2) in linkage with HLA-DRw2 as defined in white and Japanese populations. Transplant. Proc. 10: 789–791, 1978
Richiardi, P., Belvedere, M., Borelli, I., DeMarchi, M., and Curtoni, E. S.: Split of HLA-DRw2 into subtypic specificities closely correlated to two HLA-D products. Immunogenetics 7: 57–61, 1978
Ryder, L. P., Thomsen, M., Platz, P., and Svejgaard, A.: Data reduction in LD typing. In F. Kissmeyer-Nielsen (ed.): Histocompatibility Testing 1975, p.p. 557–562, Munksgaard, Copenhagen, 1975
Schaller, J. G. and Hansen, J.: Early childhood pauciarticular juvenile rheumatoid arthritis: clinical and immunogenetic studies. VIII Pan-American Congress of Rheumatology (Abstract), 1982
Sheehy, M. J., Sondel, P. M., Bach, M. L., Wank, R., and Bach, F. H.: HL-A LD (lymphocyte defined) typing: A rapid assay with primed lymphocytes. Science 188: 1308–1310, 1975
Stastny, P., Colbaugh, P., Heinle, A., Beathard, G. A., Lordon, R. E., Moraes, J. R., Olemick, S. R., and Radvany, R.: Relation of human Ia antigens with other HLA products and functions in several American populations. In W. F. Bodmer, J. R. Batchelor, J. G. Bodmer, H. Festenstein, and P. J. Morris (eds.): Histocompatibility Testing 1977, pp. 559–565, Munksgaard, Copenhagen, 1978
Suciu-Foca, N., Godfrey, M., Khan, R., Woodward, K., Rohowsky, C., Reed, E., Hardy, M., and Reemtsma, K.: New HLA-D alleles associated with DR1 and DR2. Tissue Antigens 17: 294–302, 1981
Thorsby, E. and Piazza, A.: Joint Report (MLC). II. Typing for HLA-D (LD-1 or MLC) determinants. In F. Kissmeyer-Nielsen (ed.): Histocompatibility Testing 1975, pp. 414–458, Munksgaard, Copenhagen, 1975
Troup, G. M., Jameson, J., Thomsen, M., Svejgaard, A., and Walford, R. L.: Studies of HLA alloantigens of the Navajo Indians of North America. I. Variance of association between HLA-DRW (WIA) and HLA-DW specificities. Tissue Antigens 12: 44–57, 1978
van Rood, J. J., van Leeuwen, A., Keuning, J. J., and Blusse Van Oud Alblas, A.: The serological recognition of the human MLC determinants using a modified cytotoxicity technique. Tissue Antigens 5: 73–79, 1975
Willkens, R. F., Hansen, J. A., Malmgren, J. A., Nisperos, B., Mickelson, E. M., and Watson, M. A.: HLA antigens in Yakima Indians with rheumatoid arthritis: Lack of association with HLA-Dw4 and HLA-DR4. Arthritis Rheum., in press, 1983
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Mickelson, E.M., Nisperos, B., Layrisse, Z. et al. Analysis of the HLA-DRw8 haplotype: Recognition by HTC typing of three distinct antigen complexes in Caucasians, Native Americans, and Orientals. Immunogenetics 17, 399–410 (1983). https://doi.org/10.1007/BF00372458
Received:
Revised:
Issue Date:
DOI: https://doi.org/10.1007/BF00372458