Summary
This report describes the quantification of chromosomal aberrations by flow cytometry. Both homogeneously and heterogeneously occurring chromosome aberrations were studied. Homogeneously occurring aberrations were noted in chromosomes isolated from human colon carcinoma (LoVo) cells, stained with Hoechst 33258 and chromo-mycin A3 and analyzed using dual beam flow cytometry. The resulting bivariate flow karyotype showed a homogeneously occurring marker chromosome of intermediate size. Heterogeneously occurring aberrations were quantified by slit-scan flow cytometry in chromosomes isolated from control and irradiated Chinese hamster cells and stained with propidium iodide. Heterogeneously occurring dicentric chromosomes were detected by their shapes (two centromeres). The frequencies of such chromosomes estimated by slit-scan flow cytometry correlated well with the frequencies determined by visual microscopy.
Work performed under the auspices of the U.S. Department of Energy by the Lawrence Livermore National Laboratory under contract number W-7405-ENG-48 with support from USPHS grants HD 17665 and GH 25076.
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© 1984 Springer-Verlag Berlin Heidelberg
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Gray, J.W., Lucas, J., Yu, L.C., Langlois, R. (1984). Flow Cytometric Detection of Aberrant Chromosomes. In: Eisert, W.G., Mendelsohn, M.L. (eds) Biological Dosimetry. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-69334-2_3
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DOI: https://doi.org/10.1007/978-3-642-69334-2_3
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