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Application of HPLC to the Separation of Metabolites of Nucleic Acids in Physiological Fluids

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Practice of High Performance Liquid Chromatography

Part of the book series: Chemical Laboratory Practice ((CHEMICAL LABORA))

Abstract

The last decade has witnessed an explosive growth in the interest and analysis of nucleic acids and their metabolites. Purine and pyrimidine nucleotides1, nucleosides and bases are of importance in a wide spectrum of research areas. The refinement of the instrumentation and techniques of high performance liquid chromatography (HPLC) during this decade has aided all areas of nucleic acid research1). As more reliable and rapid means of analyses are provided, many other research areas are benefitting, especially biomedical research2, 3). HPLC can be utilized in many types of research; for example, in studies of the regulatory effect of cyclic nucleotides, the determination of the composition of hydrolysates of nucleic acids, the metabolic profiling of normal and diseased subjects, and studies of disease processes. HPLC is also of great importance in the separation and purification of starting materials, intermediates, and products in the synthesis of the nucleic acids, as well as for the nucleic acids themselves. In this article we will discuss the variables involved in developing an HPLC analysis for free purine and pyrimidine compounds in biological fluids.

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Authors and Affiliations

  1. Department of Chemistry, University of Rhode Island, Kingston, 02881, RI, USA

    Anne P. Halfpenny & Phyllis R. Brown

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  1. Anne P. Halfpenny
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  2. Phyllis R. Brown
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Editors and Affiliations

  1. Angewandte Physikalische Chemie, Universität des Saarlandes, D-6600, Saarbrücken, Germany

    Heinz Engelhardt

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© 1986 Springer-Verlag Berlin · Heidelberg

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Halfpenny, A.P., Brown, P.R. (1986). Application of HPLC to the Separation of Metabolites of Nucleic Acids in Physiological Fluids. In: Engelhardt, H. (eds) Practice of High Performance Liquid Chromatography. Chemical Laboratory Practice. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-69225-3_11

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  • DOI: https://doi.org/10.1007/978-3-642-69225-3_11

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-69227-7

  • Online ISBN: 978-3-642-69225-3

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