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Practice of High Performance Liquid Chromatography

Applications, Equipment and Quantitative Analysis

  • Heinz Engelhardt

Part of the Chemical Laboratory Practice book series (CHEMICAL LABORA)

Table of contents

  1. Front Matter
    Pages I-XII
  2. Henri Colin, Georges Guiochon, Michel Martin
    Pages 1-64
  3. Jürgen Asshauer, Helmut Ullner
    Pages 65-108
  4. A. Wehrli
    Pages 109-142
  5. Herwig Hulpke, Ulrich Werthmann
    Pages 143-157
  6. M. Uihlein
    Pages 159-178
  7. J. C. Kraak, J. P. Crombeen
    Pages 179-199
  8. B. A. Persson, B. L. Karger
    Pages 201-214
  9. Hermann J. Möckel
    Pages 215-240
  10. T. Daldrup, P. Michalke, S. Szathmary
    Pages 241-285
  11. K. Aitzetmüller
    Pages 287-321
  12. H. Engelhardt
    Pages 409-425
  13. Back Matter
    Pages 455-464

About this book

Introduction

During its short 20 year history High Performance Liquid Chro­ matography (HPLC) has won itself a firm place amongst the instrumental methods of analysis. HPLC has caused a revolution in biological and pharmaceutical chemistry. Approximately two thirds of the publications on HPLC are concerned with problems from this area of life science. Biotechnology, where it is necessary to isolate substances from complicated mixtures, is likely to give further impetus to the dissemination of modern liquid chromatog­ raphy in columns, particularly on the preparative scale. This book presents, by means of examples, the application of HPLC to various fields, as well as fundamental discussions of chromatographic methods. The quality of the analytical result is decisively dependent on the qualities of the equipment employed (by Colin, Guiochon, and Martin). Especially the demands are discussed that are placed on the components of the instrument including those for data acquisition and processing. The section on "quantitative analy­ sis" (by ABhauer, Ullner) covers besides the principles also the problems of ensuring the quality of the data in detail. The basic problems arising by enlarging the sample size to preparative di­ mensions and the requirements put on the aparatus are discussed in the section on "preparative applications" (by Wehrli).

Keywords

Applications Chromat Performance amino acid biological chemistry chromatography evolution fields inorganic chemistry nucleic acid pharmaceutical chemistry protein quality quantitative analysis

Editors and affiliations

  • Heinz Engelhardt
    • 1
  1. 1.Angewandte Physikalische ChemieUniversität des SaarlandesSaarbrückenGermany

Bibliographic information

  • DOI https://doi.org/10.1007/978-3-642-69225-3
  • Copyright Information Springer-Verlag Berlin Heidelberg 1986
  • Publisher Name Springer, Berlin, Heidelberg
  • eBook Packages Springer Book Archive
  • Print ISBN 978-3-642-69227-7
  • Online ISBN 978-3-642-69225-3
  • Series Print ISSN 0172-4967
  • Buy this book on publisher's site