Advertisement

Functional Analysis of I1-2 Produced by T-Cell Hybridomas: I1-2 Promotes T-Cell Growth But Does Not Mediate T-Cell or B-Cell Maturation and Differentiation

  • K. Pfizenmaier
  • M. Röllinghoff
  • H. Wagner
Conference paper
Part of the Current Topics in Microbiology and Immunology book series (CT MICROBIOLOGY, volume 100)

Abstract

In vitro stimulation of mixtures of T-cells and macrophages/-dendritic cells with either antigen or mitogen results in the production of a variety of mediators involved in lymphocyte activation. Supernatants (Sn) of such cultures have been used for many years as a rich source of nonantigen-specific helper factors for both B-cell- and T-cell- responses (1–5). Interleukin-2 (I1-2), a factor with a mol. wt. of approximately 30 000, has been identified as a major constituent of such Sn (6–9). The characteristic feature of I1-2 is its capacity to promote T-cell growth. In addition, other biological activities were also found in I1-2-rich preparations of ConA-stimulated mouse spleen cells including B-cell stimulatory activity as well as maturation and differentiation of both pre-T-cells and thymocytes into cytotoxic effector T-cells (9–14). Because these activities copurified under conventional separation procedures, it was suggested that I1-2 is critically involved in all these processes. However, whether or not I1-2 is the only mediator remained unclear. Thus, it is conceivable that other, so far unidentified factor(s) of a similar biochemical nature contaminate the I1-2 preparations even when vigorous separation procedures are applied. These putative factor (s) may, either by themselves or synergistic with I1-2, be responsible for the distinct biological activities detected in different assay systems.

Keywords

Spleen Cell Cytotoxic Effector Percent Specific Lysis Plaque Form Cell Response Allogeneic Stimulator Cell 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Waldmann H, Munro AJ (1973) T cell-dependent mediator in the immune response. II. Physical and biological properties. Immunology 27: 53–64Google Scholar
  2. 2.
    Schimpl A, Wecker E (1975) A third signal in B cell activation given by TRF. Transplant Rev 23: 176–188PubMedGoogle Scholar
  3. 3.
    Altman A, Cohen IR (1975) Cell-free media of mixed lymphocyte cultures augmenting sensitization in vitro of mouse T lymphocytes against allogeneic fibroblasts. Eur J Immunol 5: 437–444CrossRefGoogle Scholar
  4. 4.
    Plate JMD (1976) Soluble factors substitute for T-T cell collaboration in generation of T-killer lymphocytes. Nature 260: 329–331PubMedCrossRefGoogle Scholar
  5. 5.
    Wagner H and Röllinghoff M (1978) T-T cell interactions during in vitro cytotoxic allograft responses. I. Soluble products from activated Ly 1+ T cells trigger autonomously antigen primed Ly 23+ T cells to cell proliferation and cytolytic activity. J Exp Med 148: 1523–1538PubMedCrossRefGoogle Scholar
  6. 6.
    Watson J, Gillis S, Marbrook J, Mochizuki D, Smith KA (1979) Biochemical and biological characterization of lymphocyte regulatory molecules. I. Purification of a class of murine lymphokines. J Exp Med 150: 849–861PubMedCrossRefGoogle Scholar
  7. 7.
    Wagner H, Hardt C, Heeg K, Pfizenmaier K, Solbach W, Bartlett R, Stockinger H, Röllinghoff M (1980) T-T cell interactions during cytotoxic T lymphocyte (CTL) responses: T cell derived helper factor (interleukin 2) as a probe to analyse CTL responsiveness and thymic maturation of CTL progenitors. Immunol Rev 51: 215–256PubMedCrossRefGoogle Scholar
  8. 8.
    Smith KA (1980) T cell growth factor. Immunol Rev 51: 337–357PubMedCrossRefGoogle Scholar
  9. 9.
    Watson J, Mochizuki D (1980) Interleukin 2: A class of T cell growth factors. Immunol Rev 51: 257–278PubMedCrossRefGoogle Scholar
  10. 10.
    Stötter H, Rüde E, Wagner H (1980) T cell factor (Interleukin-2) allows in vivo induction of T helper cells against heterologous erythrocytes in athymic (nu/nu) mice. Eur J Immunol 10: 719–722PubMedCrossRefGoogle Scholar
  11. 11.
    Wagner H, Hardt C, Bartlett R, Röllinghoff M, Pfizenmaier K (1980) The CTL immunocompetence of peanut agglutinin-positive (cortical) and negative (medullary) Lyt 123 thymocytes. J Immunol 125: 2532–2538PubMedGoogle Scholar
  12. 12.
    Gillis S, Union N, Baker P, Smith K (1979) The in vitro generation and sustained culture of nude mouse cytolytic T-lymphocytes. J Exp Med 149: 1460–1476PubMedCrossRefGoogle Scholar
  13. 13.
    Hunig T, Bevan MJ (1980) Specificity of cytotoxic T cells from athymic mice. J Exp Med 152: 688–702PubMedCrossRefGoogle Scholar
  14. 14.
    Wagner H, Hardt C, Bartlett R, Pfizenmaier K, Rôllinghoff M, Heeg, K (1980) T-lymphocyte progenitors from thymus deficient (nu/nu) mice differentiate in vitro into H-2 restricted, hapten-specific cytotoxic effector cells. Behring Inst Mitt 67: 105–109Google Scholar
  15. 15.
    Pfizenmaier K, Rôllinghoff M, Wagner H (1980) Establishment of hybridomas producing I1-2. Behring Inst Mitt 67: 68–74Google Scholar
  16. 16.
    Pfizenmaier K (1982) Interleukin-producing T-cell hybridomas: A biological characterization of mediators controlling lymphocyte activation. Lymphokines, 5: 323–352Google Scholar
  17. 17.
    Schimpl A, Hiibner L, Wong C, Wecker W (1980) Distinction between T helper cell replacing factor (TRF) and T cell growth factor (TCGF). Behring Inst Mitt 67: 221–235Google Scholar
  18. 18.
    Leibson H, Marrack P, Kappler IW (1981) B cell helper factors. I. Requirement for both Interleukin 2 and another 40 000 mol. wt. factor. J exp Med 154: 1681–1693PubMedCrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1982

Authors and Affiliations

  • K. Pfizenmaier
  • M. Röllinghoff
  • H. Wagner

There are no affiliations available

Personalised recommendations