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T Cell Hybridomas

A Workshop at the Basel Institute for Immunology

  • H. V. Boehmer
  • W. Haas
  • G. Köhler
  • F. Melchers
  • J. Zeuthen
Conference proceedings

Part of the Current Topics in Microbiology and Immunology book series (CT MICROBIOLOGY, volume 100)

Table of contents

  1. Front Matter
    Pages I-XI
  2. K. Fischer Lindahl, A. A. Nordin, M. H. Schreier
    Pages 1-10
  3. Z. Eshhar, T. Waks, T. Oren, G. Berke, Y. Kaufmann
    Pages 11-18
  4. E. J. Culbert, S. Kontiainen, L. M. Douglas, M. Feldmann
    Pages 19-32
  5. M. J. Taussig, G. L. Asherson, A. Holliman, N. Richardson, G. Skibinski
    Pages 43-52
  6. D. R. Webb, B. A. Araneo, C. Healy, J. A. Kapp, K. Krupen, I. Nowowiejski et al.
    Pages 53-59
  7. M. E. Dorf, K. Okuda, M. Minami
    Pages 61-67
  8. M. Cramer, R. Mierau, T. Takemori, G. Suzuki, T. Tada
    Pages 69-77
  9. M. Suemura, K. Sugimura, A. Ishizaka, S. Kobatake, O. Shiho, H. Deguchi et al.
    Pages 79-88
  10. P. Ricciardi-Castagnoli, F. Robbiati, E. Barbanti, G. Doria, L. Adorini
    Pages 89-96
  11. P. Lonai, E. Arman, S. Bitton-Grossfeld, J. Grooten, G. Hämmerling
    Pages 97-102
  12. S. Carel, C. Bron, G. Corradin
    Pages 111-116
  13. A. M. Schmitt-Verhulst, M. A. Cooley, A. Guimezanes, C. De Préval, F. Albert, M. Buferne
    Pages 125-134
  14. C. Corbel, J. Andersson, F. Melchers, J. Zeuthen
    Pages 173-178
  15. E. C. DeFreitas, S. Vella, A. Linnenbach, C. M. Croce, H. Koprowski
    Pages 191-201
  16. J. F. A. P. Miller, P. L. Mottram, W. R. Thomas, J. McKimm-Breschkin, I. L. Walker, J. Gamble
    Pages 249-255
  17. Back Matter
    Pages 257-264

About these proceedings

Introduction

For more than ten years cell fusion techniques have been applied in studies on various lymphocyte functions. Ig expression was first studied in hybrids obtained by fusing myeloma cells with fibroblasts (1) or lymphomas (2), both of which do not produce Ig, and with Ig­ producing myelomas (3) or human blood lymphocytes (4). Kohler and Milstein (5) fused a myeloma with spleen cells from immunized mice. Up to 10% of the hybrids obtained secreted antibodies specific for the immunizing antigen. This suggested that plasma cells preferenti­ ally fused with the myeloma cells, a finding which was of enormous practical value. It was found that both Band T lymphocytes could be fused with the T cell tumor BW5147, which is however not permissive for Ig synthesis (6). A very large number of T cell hybridomas were generated by fusing BW5147 with cell populations containing in vivo or in vitro activated cells (7). The hybrids showed no specific T cell functions and binding assays for T cell receptors were not available. In particular, no hybrids were obtained which expreS1ed specific cytolytic activity that could be tested in short-term Cr­ release assays (8). However, the frustrations expressed about these failures, published in January, 1978 (9), were relieved by Taniguchi and Miller's publication a few months later of T cell hybridomas producing antigen-specific suppressor factors (10). Unfortunately, their hybrids rapidly lost factor production.

Keywords

Antigen T cell antibody blood cell lymphocytes lymphoma tumor

Editors and affiliations

  • H. V. Boehmer
    • 1
  • W. Haas
    • 1
  • G. Köhler
    • 1
  • F. Melchers
    • 1
  • J. Zeuthen
    • 1
  1. 1.Basel Institute for ImmunologyBaselSwitzerland

Bibliographic information

  • DOI https://doi.org/10.1007/978-3-642-68586-6
  • Copyright Information Springer-Verlag Berlin Heidelberg 1982
  • Publisher Name Springer, Berlin, Heidelberg
  • eBook Packages Springer Book Archive
  • Print ISBN 978-3-642-68588-0
  • Online ISBN 978-3-642-68586-6
  • Series Print ISSN 0070-217X
  • Buy this book on publisher's site