Abstract
The influx of ions into mitochondria may often be demonstrated most simply, in a qualitative manner, by observing the osmotic behavior of mitochondria in a solution containing the specific ion. Mitochondria (respiration-inhibited) are suspended in a salt solution which has the same osmolarity as the mitochondrial matrix compartment (about 300 mosmolar); any influx of salt will be accompanied by an influx of water and the mitochondria will consequently increase in volume. Mitochondrial swelling is accompanied by a decrease in the light scattering of the suspension and this may be monitored spectrophotometrically.
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© 1979 Springer-Verlag New York Inc.
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Crompton, M., Chappell, J.B. (1979). Anion Transport in Mitochondria. In: Carafoli, E., Semenza, G. (eds) Membrane Biochemistry. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-67530-0_10
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DOI: https://doi.org/10.1007/978-3-642-67530-0_10
Publisher Name: Springer, Berlin, Heidelberg
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