Membrane Biochemistry

1. Front Matter

   Pages i-x

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2. Nonelectrolyte Transport in Small Intestinal Membrane Vesicles. The Application of Filtration for Transport and Binding Studies

   • Markus Kessler, Gerhard Toggenburger

   Pages 1-24

3. Transport of Sugars in Bacteria

   • Adam Kepes

   Pages 25-35

4. Net Na+ and K+ Movements in Human Red Blood Cells After Cold Storage

   • Hans-Jürg Schatzmann, Peter Ronner, Verena Niggli

   Pages 36-40

5. Calcium Transport in Resealed Erythrocytes and the Use of a Calcium-Sensitive Electrode

   • Peter Ronner

   Pages 41-50

6. Calcium Transport in Sarcoplasmic Reticulum Vesicles Isolated from Rabbit Skeletal Muscle

   • Michele Chiesi, Anthony Martonosi

   Pages 51-61

7. Preparation and Assay of Animal Mitochondria and Submitochondrial Vesicles

   • Paolo Gazzotti, Kerstin Malmström, Martin Crompton

   Pages 62-76

8. Measurement of Cytochrome Kinetics in Rat Liver Mitochondria by Stopped Flow and Dual-Wavelength Spectrophotometry

   • Angelo Azzi, Hubert Affolter

   Pages 77-81

9. Proton Translocation Catalyzed by Mitochondrial Cytochrome Oxidase

   • Mårten Wikström, Erwin Sigel

   Pages 82-91

10. Determination of the Membrane Potential and pH Difference Across the Inner Mitochondrial Membrane

    • J. Brian Chappell, Martin Crompton

    Pages 92-97

11. Anion Transport in Mitochondria

    • Martin Crompton, J. Brian Chappell

    Pages 98-102

12. Calcium Transport in Mitochondria

    • Kerstin Malmström, Ernesto Carafoli

    Pages 103-112

13. Redox Intermediates Between O2 and H2O

    • Christoph Richter

    Pages 113-119

14. Photophosphorylation with Chromatophore Membranes from Rhodospirillum Rubrum

    • Kurt W. Hanselmann, Walter Beyeler, Christian Pflugshaupt, Reinhard Bachofen

    Pages 120-143

15. Oxygen Evolution and Uptake as a Measure of the Light-Induced Electron Transport in Spinach Chloroplasts

    • Andres Binder, Reinhard Bachofen

    Pages 144-153

16. The Function of the Purple Membrane in Halobacterium Halobium

    • Dieter Oesterhelt, Rainer Hartmann

    Pages 154-163

17. Characterization of Ionophores Using Artificial Lipid Membranes

    • Michele Chiesi

    Pages 164-170

18. Characterization of Neutral and Charged Ionophores Using Vesicular Artificial Lipid Membranes (Liposomes)

    • Paolo Gazzotti, Alec D. Bangham

    Pages 171-175

This manual collects in the form of laboratory protocols a series of experiments in the field of Membrane Transport and Membrane Bioenergetics. It represents the experience accumulated during four advanced courses held at the Depart­ ment of Biochemistry of the Swiss Federal Institute of Technology on behalf of Federation of European Biochemical Societies (FEBS) in the years 1975 through 1978. The idea of collecting the experiments into a laboratory manual developed as a response to a demand from the students who took part in the courses. Further motivation came with the fmding that, in planning the laboratory sessions, the teaching staff had no organized, modern source of information in the literature. The experiments presented cover most areas of importance in the subject mat­ ter. Their presentation has been continuously modified in the course of the four years during which the manual took shape, to accommodate to experience and various suggestions. In their present form, all of the experiments described have been repeatedly practiced to optimize their execution. Efforts have been made to combine in the manual classical experiments, and techniques which require relatively unsophisticated instrumentation and can therefore be carried out in most laboratories, with more modern experiments and relatively newer technol­ ogies. In its present form, the manual should therefore provide a usefui tool in the hands of researchers and laboratory teachers at different levels of sophisti­ cation and instrumentation.

• Calcium
• Chloroplast
• Filtration
• Lipid
• Membrane
• Membrantransport
• biochemistry
• instrumentation

• Laboratory of Biochemistry, Swiss Federal Institute of Technology (ETH), Zurich, Switzerland

  Ernesto Carafoli, Giorgio Semenza

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