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Plating of Plant Cells

  • Conference paper
Plant Tissue Culture and Its Bio-technological Application

Part of the book series: Proceedings in Life Sciences ((LIFE SCIENCES))

Abstract

Plating plant cells is a technique by which a suspension of single cells is mixed with melted agar-medium at a temperature between 30° and 35°C, and spread out in petri dishes in a way that the cells are evenly distributed and fixed in a thin agar layer after the agar has cooled and solidified. If an appropriate nutrient medium is used and the cells are handled with care small colonies of single cell origin will grow from the plated cells (2). The method, first described in 1960, has since been successfully applied in the isolation of cell clones from a wide array of higher plants. It permits the screening of large numbers of cells for genetic and physiological studies and can be of importance in elucidating the biochemistry and genetics of higher plants (for instance see 32, 33, 39).

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References

  1. Beisenherz, W.: Schnellmarkierte RNS in heterotroph und mixotroph kultivierten Geweben von Nicotiana tabacum. Thesis, Köln, 1972

    Google Scholar 

  2. Beisenherz, W.: Schnellmarkierte RNS in heterotroph kultivierten Geweben von Nicotiana tabacum. Z. Naturforsch. 27b, 1205–1215 (1972)

    Google Scholar 

  3. Bergmann, L.: Growth and division of single cells of higher plants in vitro. J. Gen. Physiol. 43, 841–851 (1960)

    Article  PubMed  CAS  Google Scholar 

  4. Bergmann, L.: Wachstum grüner Suspensionskulturen von Nicotiana tabacum var. “Samsun” mit CO2 als Kohlenstoff quelle. Planta (Berl.) 74, 243–249 (1967)

    Article  CAS  Google Scholar 

  5. Bergmann, L., Bälz, A.: Der Einfluß von Farblicht auf Wachstum und Zusammensetzung pflanzlicher Gewebekulturen. Planta (Berl.) 70, 285–303 (1966)

    Article  CAS  Google Scholar 

  6. Bergmann, L., Große, W., Koth, P.: Einfluß von Ammonium und Nitrat auf Stickstoff-metabolismus, Malatanhäufung und Malatenzym-Aktivität in Suspensionskulturen von Nicotiana tabacum var. “Samsun”. Z. Pflanzenphysiol. 80, 60–70 (1976)

    CAS  Google Scholar 

  7. Bergmann, L., Rennenberg, H.: Growth inhibition of soybean callus tissues by glutathione. Naturwissenschaften 62, 349 (1975)

    Article  CAS  Google Scholar 

  8. Berlyn, M.B., Zelitsch, J.: Photoautotrophic growth and photosynthesis in tobacco callus cells. Plant Physiol. 56, 752–756 (1975)

    Article  PubMed  CAS  Google Scholar 

  9. Blakely, L.M., Steward, F.G.: Growth and organized development of cultured cells. V. The growth of colonies from free cells on nutrient agar. Am. J. Botany 51, 780–791 (1964)

    Article  Google Scholar 

  10. Davey, M.R., Street, H.E.: Studies on the growth in culture of plant cells. IX. Additional features of the fine structure of Acer pseudoplatanus L. cells cultured in suspension. J. Exp. Botany 22, 90–95 (1971)

    Article  CAS  Google Scholar 

  11. Duc Bui-Dank-Ha, Mackenzie, J.: The division of protoplasts from Asparagus officinalis L. and their growth and differentiation. Protoplasma 78, 215–221 (1973)

    Article  Google Scholar 

  12. Eagle, H.: Amino acid metabolism in mammalian cell cultures. Science 130, 432–437 (1959)

    Article  PubMed  CAS  Google Scholar 

  13. Eagle, H., Piez, K.: The population-dependent requirement by cultured mammalian cells for metabolites which they can synthesize. J. Exp. Med. 116, 29–43 (1962)

    Article  PubMed  CAS  Google Scholar 

  14. Eagle, H., Piez, K., Levy, M.: The intracellular amino acid concentrations required for protein synthesis in cultured human cells. J. Biol. Chem. 236, 2039–2042 (1961)

    PubMed  CAS  Google Scholar 

  15. Earle, E.D., Torrey, J.G.: Colony formation by isolated convolvulus cells plated on defined media. Plant Physiol. 40, 520–528 (1965)

    Article  PubMed  CAS  Google Scholar 

  16. Edelman, J., Hanson, A.D.: Sucrose suppression of chlorophyll synthesis in carrot callus cultures. Planta (Berl.) 98, 150–155 (1971)

    Article  CAS  Google Scholar 

  17. Enzmann-Becker, G.: Plating efficiency of protoplasts of tobacco in different light conditions. Z. Naturforsch. 28C, 470–471 (1973)

    Google Scholar 

  18. Fridborg, G., Eriksson, T.: Partial reversal by cytokinin and (2-Chloroethyl)-Trimethy1ammonium chloride of nearultraviolet inhibited growth and morphogenesis in callus cultures. Physiol. Plantarum 34, 162–166 (1975)

    Article  CAS  Google Scholar 

  19. Gamborg, O.L.: The effects of amino acid and ammonium on the growth of plant cells in suspension culture. Plant Physiol. 45, 372–375 (1970)

    Article  PubMed  CAS  Google Scholar 

  20. Gamborg, O.L., Miller, R.A., Ojima, K.: Nutrient requirements of suspension cultures of soybean root cells. Exp. Cell Res. 50, 151–158 (1968)

    Article  PubMed  CAS  Google Scholar 

  21. Gamborg, O.L., Wetter, L.R.: Plant tissue culture methods. Ottawa National Research Council of Canada, 1975

    Google Scholar 

  22. Gibbs, J.L., Dougall, D.K.: The growth of single cells from Nicotiana tabacum callus tissue in nutrient medium containing agar. Exp. Cell Res. 40, 85–95 (1965)

    Article  PubMed  CAS  Google Scholar 

  23. Hanson, A.D., Edelman, J.: Secretion of photosynthetic products by carrot tissue cultures. Planta (Berl.) 98, 97–108 (1971)

    Article  CAS  Google Scholar 

  24. King, J., Hirji, R.: Amino acid transport systems of cultured soybean root cells. Can. J. Botany 53, 2088–2091 (1975)

    Article  CAS  Google Scholar 

  25. King, J., Oleniuk, F.H.: The uptake of alanine-14C by soybean root cells grown in sterile suspension culture. Can. J. Botany 51, 1109–1114 (1973)

    Article  CAS  Google Scholar 

  26. Laetsch, W.M.: Tissue culture plant. Meth. Enzymol. 23, 96–109 (1971)

    Article  Google Scholar 

  27. Logemann, H.: Der Einfluß der sichtbaren Strahlung und des Mediums auf die “Plating Efficiency” isolierter Kalluszellen von Nicotiana tabacum und die Abgabe von Cytokininen in das Medium der Kulturen. Thesis, Köln, 1973

    Google Scholar 

  28. Logemann, H., Bergmann, L.: Einfluß von Licht und Medium auf die “Plating Efficiency” isolierter Zellen aus Calluskulturen von Nicotiana tabacum var. “Samsun”. Planta (Berl.) 121, 283–292 (1974)

    Article  CAS  Google Scholar 

  29. Mackenzie, I.A., Konar, A., Street, H.E.: Cytokinins and the growth of cultured sycamore cells. New Phytol. 71, 633–638 (1972)

    Article  CAS  Google Scholar 

  30. Maretzki, A., Thorn, A.: ARginine and lysine transport in sugarcane cell suspension cultures. Biochemistry 9, 2731–2736 (1970)

    Article  PubMed  CAS  Google Scholar 

  31. Maretzki, A., Thorn, A.: Membrane transport of sugar in cell suspensions of sugarcane. Plant Physiol. 49, 177–182 (1972)

    Article  PubMed  CAS  Google Scholar 

  32. Maretzki, A., Thorn, A.: The existence of two membrane transport systems for glucose in suspensions of sugarcane cells. Biochem. Biophys. Res. Commun. 47, 44–51 (1972)

    Article  PubMed  CAS  Google Scholar 

  33. Melchers, G.: Genetik und Pflanzenzüchtung mit mikrobiologischen Methoden. Planta Medica 27 (Suppl) 5–32 (1975)

    Article  Google Scholar 

  34. Melchers, G., Labib, G.: Somatic hybridisation of plants by fusion of protoplasts. I. Selection of light resistant hybrids of “Haploid” light sensitive varieties of tobacco. Mol. Gen. Genet. 135, 277–294 (1974)

    Article  Google Scholar 

  35. Murashige, T., Skoog, F.: A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plantarum 15, 473–497 (1962)

    Article  CAS  Google Scholar 

  36. Neumann, K.H., Raafat, A.: Further studies on the photosynthesis of carrot tissue cultures. Plant Physiol. 51, 685–690 (1973)

    Article  PubMed  CAS  Google Scholar 

  37. Oaks, A., Bidwell, R.G.S.: Compartmentation of intermediary metabolites. Ann. Rev. Plant Physiol. 21, 43–66 (1971)

    Article  Google Scholar 

  38. Pamplin, E.J., Chapman, J.M.: Sucrose suppression of chlorphyll synthesis in tissue culture: Changes in the activity of the enzymes of the chlorophyll bio-synthetic pathway. J. Exp. Botany 26, 212–220 (1975)

    Article  CAS  Google Scholar 

  39. Rennenberg, H.: Glutathione in conditioned media of tobacco suspension cultures. Phytochemistry 15, 1433–1434 (1976)

    Article  CAS  Google Scholar 

  40. Rice, T.B., Carlson, P.S.: Genetic analysis and plant improvement. Ann. Rev. Plant Physiol. 26, 279–308 (1975)

    Article  CAS  Google Scholar 

  41. Sargent, P.A., King, J.: Investigations of growth-promoting factors in conditioned soybean root cells and in the liquid medium in which they grow: ammonium, glutamine, and amino acids. Can. J. Botany 52, 1747–1755 (1974)

    Article  CAS  Google Scholar 

  42. Seibert, M., Wetherbee, P.J., Job, D.D.: The effects of light intensity and spectral quality on growth and shoot initiation in tobacco callus. Plant Physiol. 56, 130–139 (1975)

    Article  PubMed  CAS  Google Scholar 

  43. Simpkins, I., Street, H.E.: Studies on the growth in culture of plant cells. VII. Effects of kinetin on the carbohydrate and nitrogen metabolism of Acer pseudoplatanus L. cells grown in suspension culture. J. Exp. Botany 21, 170–185 (1970)

    Article  CAS  Google Scholar 

  44. Stickland, R.G., Sunderland, N.: Photocontrol of growth, and of anthocyanin and chlorogenic acid production in cultured callus tissues of Haplopappus gracilis. Ann. Botany (London) 36, 671–685 (1972)

    CAS  Google Scholar 

  45. Street, H.E., (ed.): Plant Tissue and Cell Culture. London: Blackwell Scientific, 1973

    Google Scholar 

  46. Stuart, R., Street, H.E.: Studies on the growth in culture of plant cells. IV. The initiation of division in suspensions of stationary-phase cells of acer pseudoplatanus L. J. Exp. Botany 20, 556–571 (1969)

    Article  Google Scholar 

  47. Stuart, R., Street, H.E.: Studies on the growth in culture of plant cells. X. Further studies on the conditioning of culture media by suspensions of Acer pseudoplatanus L. cells. J. Exp. Botany 22, 96–105 (1971)

    Article  CAS  Google Scholar 

  48. Thomas, E., Davey, M.R.: From Single Cells to Plants. London: Wykeham, 1975

    Google Scholar 

  49. Torrey, J.G., Reinert, J.: Suspension cultures of higher plant cells in synthetic media. Plant Physiol. 36, 483–491 (1961)

    Article  PubMed  CAS  Google Scholar 

  50. Uchimiya, H., Murashige, T.: Evaluation of parameters in the isolation of viable protoplasts from cultured tobacco cells. Plant Physiol. 54, 936–944 (1974)

    Article  PubMed  CAS  Google Scholar 

  51. Venketeswaran, S.: Studies on the isolation of green pigmented callus tissues of tobacco and its continued maintenance in suspension cultures. Physiol. Plantarum 18, 776–789 (1965)

    Article  Google Scholar 

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Authors
  1. L. Bergmann
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Editor information

Editors and Affiliations

  1. Lehrstuhl für Biochemie der Pflanzen, Westfälische Wilhelms-Universität, 4400, Münster, Germany

    W. Barz

  2. Lehrstuhl für Pharmazeutische Biologie der Universität Auf der Monrgenstelle, 7400, Tübingen, Germany

    E. Reinhard

  3. Lehrstuhl für Pflanzenphysiologie, Ruhr-Universität Bochum, 4630, Bochum-Querenburg, Germany

    M. H. Zenk

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© 1977 Springer-Verlag Berlin Heidelberg

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Bergmann, L. (1977). Plating of Plant Cells. In: Barz, W., Reinhard, E., Zenk, M.H. (eds) Plant Tissue Culture and Its Bio-technological Application. Proceedings in Life Sciences. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-66646-9_18

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  • DOI: https://doi.org/10.1007/978-3-642-66646-9_18

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-66648-3

  • Online ISBN: 978-3-642-66646-9

  • eBook Packages: Springer Book Archive

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