Abstract
Determination of the complete nucleotide sequence of the human genome is a goal that has excited the imaginations of scientists for nearly a decade. Knowledge of the sequence of entire chromosomes as well as of the genome itself will provide a description of all human genes and enhance our ability to detect sequence variations among individuals. Together with genetic mapping data about phenotypes, this knowledge will form the basis for systematically identifying genes underlying human diseases. In particular, the identification of genes involved in common diseases with polygenetic inheritance is a challenge for the genome project. Substantial information about the function of many genes will be revealed through comparison with genes from other organisms. Assembly of continuous chromosomal sequence will also provide resources for addressing questions related to the large-scale structure and organization of the genome.
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Platzer, M., Nyakatura, G., Rosenthal, A. (2000). Large-Scale Genomic Sequencing Using Four Color Fluorescent Detection - Principles and Protocols. In: Kessler, C. (eds) Nonradioactive Analysis of Biomolecules. Springer Lab Manuals. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-57206-7_58
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DOI: https://doi.org/10.1007/978-3-642-57206-7_58
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