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Nonradioactive Analysis of Biomolecules

  • Christoph Kessler

Part of the Springer Lab Manuals book series (SLM)

Table of contents

  1. Front Matter
    Pages I-XVII
  2. Introduction

  3. Standard Nonradioactive Labeling Systems

    1. Front Matter
      Pages 39-39
    2. Christoph Kessler
      Pages 41-58
    3. The DIG (Digoxigenin:Anti-Digoxigenin) System

      1. Hans-Joachim Höltke, Kelly King, Cathy Hoover, Irmgard Obermaier, Barbara RÜger, Klaus MÜhlegger et al.
        Pages 61-93
      2. Anton Haselbeck, Wolfgang HÖsel
        Pages 94-110
    4. The Biotin (Biotin:Streptavidin) System

      1. Ayoub Rashtchian, Jesse Mackey
        Pages 111-136
      2. Thomas Metzler, Hoeltke Hans-Joachim
        Pages 137-154
      3. Edward A. Bayer, Meir Wilchek
        Pages 155-167
    5. 5-BrdU, AAF, Gold Labeling

      1. Jean-Luc Guesdon
        Pages 168-179
      2. Jean-luc Guesdon, Jacques Auriol, Danièle Chevrier
        Pages 180-192
      3. F. E. M. Peter van de Plas, Jan L. M. Leunissen
        Pages 193-205
    6. Direct Enzyme Label

      1. James E. Marich, Jerry L. Ruth
        Pages 216-224
  4. Specialized Nonradioactive Detection Systems

    1. Front Matter
      Pages 225-225
    2. Christoph Kessler
      Pages 227-239
    3. Hans-Joachim Guder, Dieter Heindl, Hans-Peter Josel
      Pages 240-248
    4. Colorimetric Systems

      1. Hans-Joachim Guder, Dieter Heindl, Hans-Peter Josel
        Pages 249-252
      2. Peter SchÜssler, Christoph G. Grevelding, Werner Kunz
        Pages 253-257
    5. Luminescence Systems

      1. Dieter Heindl, Hans-Peter Josel
        Pages 258-261
      2. Irena Bronstein, Larry J Kricka
        Pages 262-270
      3. John H. Kenten
        Pages 271-275
      4. Dusica Gabrijelcic-Geiger, Werner Miska, Reinhard Erich Geiger
        Pages 276-283
    6. Fluorescence Systems

      1. Dieter Heindl, Hans-Peter Josel
        Pages 284-288
      2. Eleftherios P. Diamandis, Theodore K. Christopoulos
        Pages 289-294
  5. Amplification Systems for Sensitivity Enhancement

    1. Front Matter
      Pages 295-295
    2. Christoph Kessler
      Pages 297-310
    3. PCR Target Amplification

    4. Strand Displacement Amplification (SDA) Target Amplification

      1. Catherine A. Spargo, Cheryl H. Dean, Colleen M. Nycz, G. Terrance Walker
        Pages 356-366
      2. Robert A. Reid, G. Terrance Walker
        Pages 367-373
    5. Transcription-Mediated Amplification (TMA) Target Amplification

      1. Steven T. Brentano, Sherrol H. Mcdonough
        Pages 374-380
    6. Ligase Chain Reaction (LCR) Amplification

      1. George H. Jr Shimer, Keith C. Backman
        Pages 381-387
    7. Signal Amplification

      1. Mickey Urdea, Linda Wuestehube
        Pages 388-395
  6. Application Formats

    1. Front Matter
      Pages 417-417
    2. Christoph Kessler
      Pages 419-436
    3. Blot Formats: Nucleic Acids

    4. Blot Formats: Proteins and Glycoproteins

      1. Anton Haselbeck, Wolfgang HÖSel
        Pages 475-477
    5. In Situ Formats

      1. Bodil Lomholt, Sune Frederiksen, Peter E. Nielsen
        Pages 478-483
      2. Veijo Hukkanen, Pirkko Heino
        Pages 505-515

About this book

Introduction

New techniques and updated protocols for the detection and analysis of biomolecules - nucleic acids, proteins and glycoproteins - are presented in the second edition of this successful laboratory manual. Highly sensitive systems which are widely used in molecular biological and biomedical laboratories, such as colorimetric, luminescence, fluorescence measuring using antibody/antigen binding or hybridisation, as well as PCR amplification are described in detail.
The clearly structured step-by-step protocols with practical hints and a troubleshooting guide are complemented by chapters on the theoretical background and the application of the techniques. Thus the manual will enable scientists to plan, design and conduct procedures which fulfill their particular requirements.

Keywords

Activation Chemolumineszenz DNA DNA-Nachweis In Situ Hybridisierung Luminescence Nucleotide PCR Proteinnachweismethoden RNA gene expression glycoprotein proteins transcription translation

Editors and affiliations

  • Christoph Kessler
    • 1
    • 2
  1. 1.Roche Molecular Systems Werk TutzingRoche Diagnostics GmbHTutzingGermany
  2. 2.Gene Center/Institute of BiochemistryLudwig-Maximilians-Universität MünchenMünchenGermany

Bibliographic information

  • DOI https://doi.org/10.1007/978-3-642-57206-7
  • Copyright Information Springer-Verlag Berlin Heidelberg 2000
  • Publisher Name Springer, Berlin, Heidelberg
  • eBook Packages Springer Book Archive
  • Print ISBN 978-3-540-64601-3
  • Online ISBN 978-3-642-57206-7
  • Buy this book on publisher's site