Nonradioactive Analysis of Biomolecules

  • Christoph Kessler

Part of the Springer Lab Manuals book series (SLM)

Table of contents

  1. Front Matter
    Pages I-XVII
  2. Introduction

  3. Standard Nonradioactive Labeling Systems

    1. Front Matter
      Pages 39-39
    2. Christoph Kessler
      Pages 41-58
    3. The DIG (Digoxigenin:Anti-Digoxigenin) System

      1. Hans-Joachim Höltke, Kelly King, Cathy Hoover, Irmgard Obermaier, Barbara RÜger, Klaus MÜhlegger et al.
        Pages 61-93
      2. Anton Haselbeck, Wolfgang HÖsel
        Pages 94-110
    4. The Biotin (Biotin:Streptavidin) System

      1. Ayoub Rashtchian, Jesse Mackey
        Pages 111-136
      2. Thomas Metzler, Hoeltke Hans-Joachim
        Pages 137-154
      3. Edward A. Bayer, Meir Wilchek
        Pages 155-167
    5. 5-BrdU, AAF, Gold Labeling

      1. Jean-Luc Guesdon
        Pages 168-179
      2. Jean-luc Guesdon, Jacques Auriol, Danièle Chevrier
        Pages 180-192
      3. F. E. M. Peter van de Plas, Jan L. M. Leunissen
        Pages 193-205
    6. Direct Enzyme Label

      1. James E. Marich, Jerry L. Ruth
        Pages 216-224
  4. Specialized Nonradioactive Detection Systems

    1. Front Matter
      Pages 225-225
    2. Christoph Kessler
      Pages 227-239
    3. Hans-Joachim Guder, Dieter Heindl, Hans-Peter Josel
      Pages 240-248
    4. Colorimetric Systems

      1. Hans-Joachim Guder, Dieter Heindl, Hans-Peter Josel
        Pages 249-252
      2. Peter SchÜssler, Christoph G. Grevelding, Werner Kunz
        Pages 253-257

About this book

Introduction

New techniques and updated protocols for the detection and analysis of biomolecules - nucleic acids, proteins and glycoproteins - are presented in the second edition of this successful laboratory manual. Highly sensitive systems which are widely used in molecular biological and biomedical laboratories, such as colorimetric, luminescence, fluorescence measuring using antibody/antigen binding or hybridisation, as well as PCR amplification are described in detail.
The clearly structured step-by-step protocols with practical hints and a troubleshooting guide are complemented by chapters on the theoretical background and the application of the techniques. Thus the manual will enable scientists to plan, design and conduct procedures which fulfill their particular requirements.

Keywords

Activation Chemolumineszenz DNA DNA-Nachweis In Situ Hybridisierung Luminescence Nucleotide PCR Proteinnachweismethoden RNA gene expression glycoprotein proteins transcription translation

Editors and affiliations

  • Christoph Kessler
    • 1
    • 2
  1. 1.Roche Molecular Systems Werk TutzingRoche Diagnostics GmbHTutzingGermany
  2. 2.Gene Center/Institute of BiochemistryLudwig-Maximilians-Universität MünchenMünchenGermany

Bibliographic information

  • DOI https://doi.org/10.1007/978-3-642-57206-7
  • Copyright Information Springer-Verlag Berlin Heidelberg 2000
  • Publisher Name Springer, Berlin, Heidelberg
  • eBook Packages Springer Book Archive
  • Print ISBN 978-3-540-64601-3
  • Online ISBN 978-3-642-57206-7
  • About this book