Abstract
Binding between analyte and modified probe resulting in stable hybrid molecules is most prominently mediated by specific interaction between complementary purine and pyrimidine bases forming A:T and G:C pairs.
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References
Britten RJ, Graham DE, Neufeld BR (1974) Analysis of repeating DNA sequences by re-association. Methods Enzymol 29:363–418
Cook AF, Vuocolo E, Brakel CL (1988) Synthesis and hybridization of a series of biotinylated oligonucleotides. Nucleic Acids Res 16:4077–4095
Davidson EH, Britten RJ (1979) Regulation of gene expression: possible role of repetitive sequences. Science 204:1052–1059
Gebeyehu G, Rao PY, SooChan P, Simms DA, Kievan L (1987) Novel biotinylated nucleotide analoge for labeling and colorimetric detection of DNA. Nucleic Acids Res 15:4513–4534
Gillam IC, Tener GM (1986) N4-(6-aminohexyl)cytidine and -deoxycytidine nucleotides can be used to label DNA. Anal Biochem 157:199–207
Haralambidis J, Chai M, Tregear GW (1987) Preparation of base-modified nucleosides suitable for non-radioactive label attachment and their incorporation into synthetic oligodeoxyribonucleotides. Nucleic Acids Res 15:4857–4876
Hutton JR, Wetmur JG (1973) Length dependence of the kinetic complexity of mouse satellite DNA. Biochem Biophys Res Commun 52:1148–1155
Huynh Dinh T, Sarfati S, Igolen J, Guesdon JL (1987) Markers for detecting nucleic acids are derived from 2’-desoxy adenosine derivatives. Eur Pat Appl 0254646
Jablonski E, Moomaw KW, Tullis RH, Ruth JL (1986) Preparation of oligodeoxynucleotide-alkaline phosphatase conjugates and their use as hybridization probes. Nucleic Acids Res 14:6115–6128
Jablonski E, Ruth JL (1986) Synthesis of oligonucleotide-enzyme conjugates and their use as hybridization probes. DNA 5:89
Keller GH, Cumming CU, Huang DP, Manak MM, Ting R (1988) A chemical method for introducing haptens onto DNA probes. Anal Biochem 170:441–450
Keller GH, Manak MM (1989) DNA Probes. Stockton Press, New York, NY
Kohne DE, Levinson SA, Byers MJ (1977) Room temperatue method for increasing the rate of DNA reassoziation by many thousandfold: The phenol emulsion reassociation technique. Biochemistry 16:5329–5341
Langer PR, Waldrop AA, Ward DC (1981) Enzymatic synthesis of biotin-labeled polynucleotides: novel nucleic acid affinity probes. Proc Natl Acad Sci USA 78:6633–6637
Marmur J, Doty P (1959) Heterogeneity in DNA. I. Dependence on composition of the configurational stability of deoxyribonucleic acids. Nature 183:1427–1428 Meinkoth J, Wahl G (1984) Hybridization of nucleic acids immobilized on solid supports. Anal Biochem 138:1427–1428
Reisfeld A, Rothenberg JM, Bayer EA, Wilchek M (1987) Nonradioactive hybridization probes prepared by the reaction of biotin hydrazide with DNA. Biochem. Biophys Res Commun 142:519–526
Ruth JL, Morgan C, Pasko A (1985) Linker arm nucleotide analogue useful in oligonucleotide synthesis. DNA 4:93
Tchen P, Fuchs RPP, Sage E, Leng M (1984) Chemically modified nucleic acids as immunodetectable probes in hybridization experiments. Proc Natl Acad Sci USA 81:3466–3470
Thompson J, Gillespie D (1987) Molecular hybridization with RNA probes in concentrated solutions of guanidine thiocyanate. Anal Biochem 163:281–291
Urdea MS, Running JA, Horn T, Clyne J, Ku L, Warner BD (1987) A novel method for the rapid detection of specific nucleotide sequences in crude biological samples without blotting or radioactivity; application to the analysis of hepatitis B virus in human serum. Gene 61:253–264
Urdea MS, Warner BD, Running JA, Stempien M, Clyne J, Horn T (1988) A comparison of non-radioisotopic hybridization assay methods using fluorescent, chemiluminescent and enzyme labeled synthetic oligodeoxyribonucleotide probes. Nucleic Acids Res 16:4937–4956
Verdlov ED, Monastyrskaya GS, Guskova LI, Levitan TL, Sheichenko VI, Budowsky EI (1974) Modification of cytidine residues with a bisulfite-O-methylhydroxyl-amine mixture. Biochem Biophys Acta 340:153–165
Viscidi RP, Connelly CJ, Yolken RH (1986) Novel chemical method for the preparation of nucleic acids for nonisotopic hybridization. J Clin Microbiol 23:311–317
Wahl GM, Stern M, Stark GR (1979) Efficient transfer of large DNA fragments from agarose gels to diazobenzyloxymethyl-paper and rapid hybridization by using dextran sulfate. Proc Natl Acad Sci USA 76:3683–3687
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© 2000 Springer-Verlag Berlin Heidelberg
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Kessler, C. (2000). Overview on Factors Influencing Nucleic Acid Hybridization. In: Kessler, C. (eds) Nonradioactive Analysis of Biomolecules. Springer Lab Manuals. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-57206-7_37
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DOI: https://doi.org/10.1007/978-3-642-57206-7_37
Publisher Name: Springer, Berlin, Heidelberg
Print ISBN: 978-3-540-64601-3
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