Skip to main content
SpringerLink
Log in

Die stationäre Phase in der Dünnschichtchromatographie

  • Chapter
  • First Online:
Quantitative Dünnschichtchromatographie

  • 8044 Accesses

Zusammenfassung

Kieselgel ist die dominierende stationäre Phase in der Dünnschichtchromatographie. Dies ist ein wichtiger Unterschied zur HPLC, denn dort werden die meisten Trennungen verteilungschromatographisch im Reversed‐phase‐Modus durchgeführt. Trennungen an Normalphase sind dort eher selten, da in umständlicher Weise der Wassergehalt aller verwendeten Laufmittel eingestellt werden muss. Im Gegensatz zur HPLC ist die Verwendung von Normalphasensystemen in der DC mit keinerlei Problemen verbunden.

Kapitel 3 befasst sich mit den verschiedenen stationären Phasen, die in der Dünnschichtchromatographie eingesetzt werden. Die stationären Phasen werden mit ihren charakteristischen Parametern beschrieben, ihre Stärken und Schwächen werden diskutiert.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Log in via an institution
Chapter
USD 29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 129.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Hardcover Book
USD 169.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

Literatur

  1. Grinberg N (Hrsg) (1990) Modern thin-layer chromatography. Chromatographic science series, Bd 52. Marcel Dekker, New York

    Google Scholar 

  2. Snyder LR (1968) Principles of adsorption chromatography. Marcel Dekker, New York

    Google Scholar 

  3. Geiss F (1987) Fundamentals of thin-layer chromatography. Huetig, New York

    Google Scholar 

  4. Stahl E (1969) Thin-layer chromatography, a laboratory handbook. Springer, Berlin

    Book  Google Scholar 

  5. Zlatkis A, Kaiser RE (Hrsg) (1977) High performance thin-layer chromatography. Elsevier, Amsterdam

    Google Scholar 

  6. Poole CF (1988) Progress in planar chromatography. Anal Chem 4:209–213

    Google Scholar 

  7. Poole CF, Poole SK (1994) Instrumental thin-layer chromatography. Anal Chem 66:27 A–37 A

    Google Scholar 

  8. Andreev LF (1982) High performance thin-film chromatography in biological studies. J Liq Chromatogr 5:1573–1582

    Article  CAS  Google Scholar 

  9. Hauck HE, Bund O, Fischer W, Schulz M (2001) Ultrathin-layer chromatography (UTLC) – A new dimension in thin-layer chromatography. J Planar Chromatogr 14:234–236

    Article  CAS  Google Scholar 

  10. Poole SK, Ahmed HD, Belay MT, Fernando WP, Poole CF (1990) The influence of layer thickness on the chromatographic and optical properties if high-performance silica gel thin-layer chromatographic plates. J Planar Chromatogr 3:133–140

    CAS  Google Scholar 

  11. Litvinova LS, Kurenbin OI (1991) An equation expressing the effect of sorbent characteristics on eluent flow velocity in thin-layer chromatography. J Planar Chromatogr 4:402–405

    CAS  Google Scholar 

  12. Wheeler A (1955). In: Emmett PH (Hrsg) Catalysis, Bd II. Reinhold, New York

    Google Scholar 

  13. Nikolova-Damyanova B, Momchilova Sv (2005) Silver ion thin-layer chromatography of fatty acids. A Survey. J Liq Chromatogr & rel techno 24:1447–1466

    Article  Google Scholar 

  14. Wall PE (1997) Argentation HPTLC as an effective separation technique for the cis/trans isomers of capsaicin. J Planar Chromatogr 10:4–9

    CAS  Google Scholar 

  15. Wardas W, Pyka A (1998) Visualizing agents for cholesterol derivatives. J Planar Chromatogr 11:70–73

    CAS  Google Scholar 

  16. Li T, Li J, Li H (1995) Modified and convenient preparation of silica impregnated with silver nitrate and its application to the separation of steroids and triperpenes: J Chromatogr A 715:372–375

    Article  CAS  Google Scholar 

  17. Lichtentaler H, Börner K (1982) Separation of prenylquinones, prenylvitamins and prenols on thin-layer plates impregnated with silver nitrate. J Chromatogr 242:196–201

    Article  Google Scholar 

  18. Brühl L, Schulte E, und Their H-P (1994) Composition and structures of triglycerides of human milk and some base components for infant milk formulas. Fat Sci Technol 96:147–154

    Google Scholar 

  19. Aubourg SP, Sotelo CG, Gallordo JM (1990) Zonal distribution of fatty acids in Albacore (Thunnus alalunga) triglycerides and their changes during cooking. J Agric Food Chem 38:255–257

    Article  CAS  Google Scholar 

  20. Colarow L (1989) Quantification of phospholipid classes on thin-layer plates with a fluorescence reagent in the mobile phase. J Planar Chromatogr 2:19–23

    CAS  Google Scholar 

  21. Hegewald H (1996) One-dimensional thin-layer chromatography of all known D-3 and D-4 isomers of phosphoinositides. Anal Biochem 242:152–155

    Article  CAS  Google Scholar 

  22. Sempore BG, Bezard JA (1994) Separation of monoacylglycerol enantiomers as urethane derivatives by chiral-phase high-performance liquid chromatography. J liq Chromatogr 17:1679–1694

    Article  CAS  Google Scholar 

  23. Lato M, Brunelli B, Ciuffini G, Mezzetti T (1968). J Chromatogr 34:26

    Article  CAS  Google Scholar 

  24. Ruiz JI, Ochoa B (1997) Quantification in the subnanomolar range of phospholipids and neutral lipids by monodimensional thin-layer chromatography and image analysis. J Lipid Res 38:1482–1489

    CAS  Google Scholar 

  25. Bhushan R, Parshad U (1996) Separation and identification of some cephalosporins on impregnated TLC plates. Biomed Chromatogr 10:256–260

    Article  Google Scholar 

  26. Naidong W, Hua S, Roets E, Hoogmartens J (1994) Purity control of six tetracyclines by semi-quantitative TLC with fluorescence detection. J Planar Chromatogr 7:297–300

    CAS  Google Scholar 

  27. Sharma SD, Misra S, Gupta R (1993) Normal phase, reversed phase and chelation thin-layer chromatographic studies of some toxic metal ions in two component solvent systems containing DMSO: Quantitative separation of Pb2+ from Hg2 2+, Hg2+, Tl+, Bi3+, Sn4+, and Sb3+. J Liquid Chromatogr 16:1833–1843

    Article  CAS  Google Scholar 

  28. Kovács-Haday K, Balázs E (1993) Thermoanalytical study of the impregnation of silica gel layers. J Planar Chromatogr 6, 463–466

    Google Scholar 

  29. Funk W (1989) Polynuclear aromatic hydrocarbons (PAHs): Charge transfer chromatography and fluorimetric determination. J Planar Chromatogr 2:28–32

    CAS  Google Scholar 

  30. Membrado L, Cebolla VL, Matt M, Gàlvez EM, Domingo MP, Vela J, Beregovtsova N (2002) Hydrocarbon group-type analysis by thin-layer chromatography and scanning densitometry. J Planar Chromatogr 15:268–273

    Article  CAS  Google Scholar 

  31. Wan KT, Weinstein B 1972. In: Niederwieser A, Pataki G (Hrsg) Progress in thin-layer chromatography, Bd 3. Ann Arbor Scíence Publishers, Michigan

    Google Scholar 

  32. Berendsen GE, Pikart KA, de Galan L (1980) Preparation of various bonded phases for HPLC using monochlorosilanes. J Liq Chromatogr 3:1437–1464

    Article  CAS  Google Scholar 

  33. Spangenberg B, Arranz I, Stroka J, Anklam E 2003) A simple and reliable HPTLC method for the quantification of the intense sweetner Sucralose®. J Liq Chrom & rel technol 26:2729–2739

    Article  CAS  Google Scholar 

  34. Gilpin RK, Sisco WR (1976) Preparation and characterization of chemically bonded thin-layer chromatographic plates. J Chromatogr 124:257–264

    Article  CAS  Google Scholar 

  35. Ericson M, Blomberg LG (1980) Modification of HPTLC-plates by in situ chemical bonding with non-polar and polar organosilanes. J High Resolut Chromatogr Commun 3:345–350

    Article  Google Scholar 

  36. Lepri L (1997) Enantiomer separation by TLC. J Planar Chromatogr 10:320–331

    CAS  Google Scholar 

  37. Nyiredi (Hrsg) (2000) Planar chromatography, a retrospective view of the third millennium. Springer, Budapest

    Google Scholar 

  38. Perišić-Janjić N, Vujičić B (1997) Analysis of some food components by thin-layer chromatography on unconventional layers. J Planar Chromatogr 10:447–452

    Google Scholar 

  39. Bushan R, Brückner H (2004) Marfey’s reagent for chiral amino acid analysis: A review. Amino acids 27:231–247

    Article  Google Scholar 

  40. Suedee R, Songkram C, Petmoreekul A, Sangkunakup S, Sankasa S, Kongyarit N (1998) Thin-layer chromatography using synthetic polymers imprinted with quinine as chiral stationary phase. J Planar Chromatogr 11:272–276

    CAS  Google Scholar 

  41. Hahn-Deinstrop E (1992) Stationary phases, sorbents. J Planar Chromatogr 5:57–61

    CAS  Google Scholar 

  42. Randerath K (1965) Dünnschicht-Chromatographie. Verlag Chemie, Weinheim

    Google Scholar 

  43. Frey H-P, Zieloff K (1993) Qualitative und quantitative Dünnschichtchromatographie, Grundlagen und Praxis. Verlag Chemie, Weinheim

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Hochschule Offenburg FB Maschinenbau und Verfahrenstechnik, Offenburg, Deutschland

    Bernd Spangenberg

Authors
  1. Bernd Spangenberg
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Bernd Spangenberg .

Rights and permissions

Reprints and permissions

Copyright information

© 2014 Springer-Verlag Berlin Heidelberg

About this chapter

Cite this chapter

Spangenberg, B. (2014). Die stationäre Phase in der Dünnschichtchromatographie. In: Quantitative Dünnschichtchromatographie. Springer Spektrum, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-55102-4_3

Download citation

Publish with us

Policies and ethics

Search

Navigation