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VH Family Usage and Binding Analyses of Polyreactive Monoclonal Autoantibodies Derived from Nonimmunized Adult BALB/c Mice

  • Conference paper
Genetics of Immunological Diseases

Part of the book series: Current Topics in Microbiology and Immunology ((CT MICROBIOLOGY,volume 137))

  • 94 Accesses

Abstract

The presence of natural autoantibodies in the B-cell repertoire of normal healthy mice has been well established and suggests that some autoreactive B cells could be activated by the internal environment, i.e., naturally activated (Dighiero et al. 1983; Prabhakar et al. 1984; Steele and Cunningham 1978). In order to better understand the naturally activated repertoire, we have examined the reactivity patterns of a panel of BALB/c hybridomas from unmanipulated adult spleen cells. This panel of natural antibody hybridomas (NAbs), selected first for organ binding by indirect immunofluorescence microscopy, was used to examine the following questions:

  1. 1.

    Are organ-reactive NAbs polyreactive?

  2. 2.

    Is there preferential Vg usage in NAbs?

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Author information

Authors and Affiliations

  1. Department of Biologies Research, Walter Reed Army Institute of Research, Washington, DC, USA

    A. B. Hartman

  2. Laboratory of Genetics, National Cancer Institute, Bethesda, MD, USA

    C. P. Mallett & S. J. Smith-Gill

  3. Laboratory of Oral Medicine, National Institute of Dental Research, National Institutes of Health, Bethesda, MD, USA

    A. B. Hartman, J. Srinivasappa, B. S. Prabhakar & A. L. Notkins

Authors
  1. A. B. Hartman
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  2. C. P. Mallett
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  3. J. Srinivasappa
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  4. B. S. Prabhakar
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  5. A. L. Notkins
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  6. S. J. Smith-Gill
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Editor information

Editors and Affiliations

  1. Laboratory of Genetics, National Cancer Institute, National Institutes of Health, Bethesda, MD, 20892, USA

    Beverly Mock Ph.D  & Michael Potter M.D.  & 

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© 1988 Springer-Verlag Berlin · Heidelberg

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Hartman, A.B., Mallett, C.P., Srinivasappa, J., Prabhakar, B.S., Notkins, A.L., Smith-Gill, S.J. (1988). VH Family Usage and Binding Analyses of Polyreactive Monoclonal Autoantibodies Derived from Nonimmunized Adult BALB/c Mice. In: Mock, B., Potter, M. (eds) Genetics of Immunological Diseases. Current Topics in Microbiology and Immunology, vol 137. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-50059-6_31

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  • DOI: https://doi.org/10.1007/978-3-642-50059-6_31

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-50061-9

  • Online ISBN: 978-3-642-50059-6

  • eBook Packages: Springer Book Archive

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