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In‐Vitro Production of Functional RBCs from Hematopoietic Stem Cells

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Hemoglobin-Based Oxygen Carriers as Red Cell Substitutes and Oxygen Therapeutics
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Abstract

A great deal of research has focused on methods to produce red blood cells (RBCs) industrially in order to reduce reliance on the supply from human donations. Among the possible candidates for artificial blood, the most perfect substitute would be actual human RBCs derived from erythroid progenitor cells grown in vitro just as occurs in vivo.

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References

  • Giarratana MC, Kobari L, Lapillonne H, Chalmers D, Kiger L, Cynober T, Marden MC, Wajcman H, Douay L (2005) Ex vivo generation of fully mature human red blood cells from hematopoietic stem cells. Nat Biotechnol 23(1):69–74

    Article  Google Scholar 

  • Giarratana MC, Rouard H, Dumont A, Kiger L, Safeukui I, Le Pennec PY, Francois S, Trugnan G, Peyrard T, Marie T, Jolly S, Hebert N, Mazurier C, Mario N, Harmand L, Lapillonne H, Devaux JY, Douay L (2011) Proof of principle for transfusion of in vitro-generated red blood cells. Blood 118(19):5071–5079

    Article  Google Scholar 

  • Goodnough LT, Skikne B, Brugnara C (2000) Erythropoietin, iron, and erythropoiesis. Blood 96(3):823–833

    Google Scholar 

  • Gordon MY, Lewis JL, Marley SB (2002) Of mice and men…and elephants. Blood 100(13):4679–4680

    Article  Google Scholar 

  • Housler GJ, Miki T, Schmelzer E, Pekor C, Zhang X, Kang L, Voskinarian-Berse V, Abbot S, Zeilinger K, Gerlach JC (2012) Compartmental hollow fiber capillary membrane-based bioreactor technology for in vitro studies on red blood cell lineage direction of hematopoietic stem cells. Tissue Eng Part C Methods 18(2):133–142

    Article  Google Scholar 

  • Kim HO, Baek EJ (2012) Red blood cell engineering in stroma and serum/plasma-free conditions and long term storage. Tissue Eng Part A 18(1–2):117–126

    Article  Google Scholar 

  • Lapillonne H, Kobari L, Mazurier C, Tropel P, Giarratana MC, Zanella-Cleon I, Kiger L, Wattenhofer-Donze M, Puccio H, Hebert N, Francina A, Andreu G, Viville S, Douay L (2010) Red blood cell generation from human induced pluripotent stem cells: perspectives for transfusion medicine. Haematologica 95(10):1651–1659

    Article  Google Scholar 

  • Laurent LC, Ulitsky I, Slavin I, Tran H, Schork A, Morey R, Lynch C, Harness JV, Lee S, Barrero MJ, Ku S, Martynova M, Semechkin R, Galat V, Gottesfeld J, Izpisua Belmonte JC, Murry C, Keirstead HS, Park HS, Schmidt U, Laslett AL, Muller FJ, Nievergelt CM, Shamir R, Loring JF (2011) Dynamic changes in the copy number of pluripotency and cell proliferation genes in human ESCs and iPSCs during reprogramming and time in culture. Cell Stem Cell 8(1):106-118

    Google Scholar 

  • Lippi G, Montagnana M, Franchini M (2011) Ex-vivo red blood cells generation: a step ahead in transfusion medicine? Eur J Intern Med 22(1):16–19

    Article  Google Scholar 

  • Lu SJ, Feng Q, Park JS, Vida L, Lee BS, Strausbauch M, Wettstein PJ, Honig GR, Lanza R (2008) Biologic properties and enucleation of red blood cells from human embryonic stem cells. Blood 112(12):4475–4484

    Article  Google Scholar 

  • Ma F, Ebihara Y, Umeda K, Sakai H, Hanada S, Zhang H, Zaike Y, Tsuchida E, Nakahata T, Nakauchi H, Tsuji K (2008) Generation of functional erythrocytes from human embryonic stem cell-derived definitive hematopoiesis. Proc Natl Acad Sci U S A 105(35):13087–13092

    Article  Google Scholar 

  • Maitra A, Arking DE, Shivapurkar N, Ikeda M, Stastny V, Kassauei K, Sui G, Cutler DJ, Liu Y, Brimble SN, Noaksson K, Hyllner J, Schulz TC, Zeng X, Freed WJ, Crook J, Abraham S, Colman A, Sartipy P, Matsui S, Carpenter M, Gazdar AF, Rao M, Chakravarti A (2005) Genomic alterations in cultured human embryonic stem cells. Nat Genet 37(10):1099–1103

    Article  Google Scholar 

  • Manwani DBJ (2008) The erythroblastic island. Curr Top Dev Biol 82:23–53

    Article  Google Scholar 

  • Miharada K, Hiroyama T, Sudo K, Nagasawa T, Nakamura Y (2006) Efficient enucleation of erythroblasts differentiated in vitro from hematopoietic stem and progenitor cells. Nat Biotech 24(10):1255–1256

    Article  Google Scholar 

  • Thomson JA, Itskovitz-Eldor J, Shapiro SS, Waknitz MA, Swiergiel JJ, Marshall VS, Jones JM (1998) Embryonic stem cell lines derived from human blastocysts. Science 282(5391):1145–1147

    Article  Google Scholar 

  • Timmins NE, Athanasas S, Gunther M, Buntine P, Nielsen LK (2011) Ultra-high-yield manufacture of red blood cells from hematopoietic stem cells. Tissue Eng Part C Methods 17(11):1131–1137

    Article  Google Scholar 

  • Timmins NE, Nielsen LK (2011) Manufactured RBC–rivers of blood, or an oasis in the desert? Biotechnol Adv 29(6):661–666

    Article  Google Scholar 

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Correspondence to Eun Jung Baek .

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Baek, E.J., Kim, H.O. (2013). In‐Vitro Production of Functional RBCs from Hematopoietic Stem Cells. In: Kim, H., Greenburg, A. (eds) Hemoglobin-Based Oxygen Carriers as Red Cell Substitutes and Oxygen Therapeutics. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-40717-8_20

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