Abstract
Glucose sensing is an issue with great interest in medical and biological applications. One possible approach to glucose detection takes advantage of measuring changes in fluorescence resonance energy transfer (FRET) between a fluorescent donor and an acceptor within a protein which undergoes glucose-induced changes in conformation. This demands the detection of fluorescent signals in the visible spectrum. In this paper we analyzed the emission spectrum obtained from fluorescent labels attached to a protein which changes its conformation in the presence of glucose using a commercial spectrofluorometer. Different glucose nanosensors were used to measure the output spectra with fluorescent signals located at the cyan and yellow bands of the spectrum. A new device is presented based on multilayered a-SiC:H heterostructures to detect identical transient visible signals. The transducer consists of a p-i’(a-SiC:H)-n/p-i(a-Si:H)-n heterostructure optimized for the detection of the fluorescence resonance energy transfer between fluorophores with excitation in the violet (400 nm) and emissions in the cyan (470 nm) and yellow (588 nm) range of the spectrum. Results show that the device photocurrent signal measured under reverse bias and using appropriate steady state optical bias, allows the separate detection of the cyan and yellow fluorescence signals.presented.
Chapter PDF
Similar content being viewed by others
Keywords
References
Deuschle, K., Okumoto, S., Fehr, M., Looger, L.L., Kozhukh, L., Frommer, W.B.: Protein Sci. 14(9), 2304–2314 (2005)
Vieira, M.A., Vieira, M., Costa, J., Louro, P., Fernandes, M., Fantoni, A.: Sens. Transd. 9, 96–120 (2010)
Martins, S., Andrade, J., Karmali, A., Serralheiro, L.J.: Molecular Recognition 19, 340–347 (2006)
Louro, P., Vieira, M., Vieira, M.A., Fernandes, M., Fantoni, A., Francisco, C., Barata, M.: Physica E: Low-dimensional Systems and Nanostructures 41, 1082–1085 (2009)
Louro, P., Vieira, M., Fernandes, M., Costa, J., Vieira, M.A., Caeiro, J., Neves, N., Barata, M.: Phys. Status Solidi. C 7(3-4), 1188–1191 (2010)
Vieira, M., Fernandes, M., Louro, P., Fantoni, A., Vieira, M.A., Costa, J., Barata, M.: Phys. Status Solidi C 8(3), 1079–1082 (2011)
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2013 IFIP International Federation for Information Processing
About this paper
Cite this paper
Louro, P., Silva, V., Vieira, M.A., Karmali, A., Vieira, M. (2013). Use of a-SiC:H Semiconductor-Based Transducer for Glucose Sensing through FRET Analysis. In: Camarinha-Matos, L.M., Tomic, S., Graça, P. (eds) Technological Innovation for the Internet of Things. DoCEIS 2013. IFIP Advances in Information and Communication Technology, vol 394. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-37291-9_68
Download citation
DOI: https://doi.org/10.1007/978-3-642-37291-9_68
Publisher Name: Springer, Berlin, Heidelberg
Print ISBN: 978-3-642-37290-2
Online ISBN: 978-3-642-37291-9
eBook Packages: Computer ScienceComputer Science (R0)