Abstract
Arbuscular mycorrhizal (AM) fungi are very particular micro-organisms concerning their cytology, their genetics and their ecology. Being obligate biotrophs they are difficult objects for research. Modern molecular methods have, however, allowed elucidating the biology during their life cycle. This chapter first describes experimental systems and approaches from the first in vitro cultures up to recent laser dissection microscopy. The second part summarises how the application of these methodologies resulted in today’s knowledge about AM fungal functioning at four developmental stages: asymbiotic spore germination, presymbiotic hyphal branching, mutualistic exchange of nutrients with the plant and the adaptations of extraradical hyphae to their environment.
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Acknowledgements
Astrid Waschke was supported by the DFG (German Research Foundation: SFB 395) and by the Ministries of Food, Agriculture, and Consumer Protection of the Federal Republic of Germany and of the states Brandenburg and Thüringen.
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Franken, P., Waschke, A., Requena, N. (2012). 2 Molecular Approaches to Arbuscular Mycorrhiza Functioning. In: Hock, B. (eds) Fungal Associations. The Mycota, vol 9. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-30826-0_2
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