Abstract
Many research projects require the localization of certain cellular structures, especially organelles. Specific organelle markers (endoplasmic reticulum, golgi apparatus, endosomes, lysosomes, nucleus, plasma membrane, mitochondria, peroxisome and centromer) including antibodies to organelle-specific proteins are helpful to locate these organelles by immunocytochemical or histological techniques. Many of the fluorescent probes designed for selecting organelles are able to permeate or sequester within the cell membrane (and therefore are useful in living cells), while others must be installed using monoclonal antibodies with traditional immunocytochemistry techniques (http://probes.invitrogen.com/handbook). The website http://www.organell-marker.de/powered by Acris Antibodies GmbH (Herford, Germany; http://www.acris-antibodies.com/) simplifies the search for organelle markers.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Capani F, Deerinck TJ, Ellisman MH, Bushong E, Bobik M, Martone ME (2001) Phalloidin-eosin followed by photo-oxidation: a novel method for localizing F-actin at the light and electron microscopic levels. J Histochem Cytochem 49:1351–1361
Cohen M, Lee KK, Wilson KL, Gruenbaum Y (2001) Transcriptional repression, apoptosis, human disease and the functional evolution of the nuclear lamina. Trends Biochem Sci 26(1):41–47
Cooper JA (1987) Effects of cytochalasin and phalloidin on actin. J Cell Biol 105:1473–1478
Dechat T, Korbei B, Vaughan OA, Vlcek S, Hutchison CJ, Foisner R (2000) Lamina-associated polypeptide 2alpha binds intranuclear A-type lamins. J Cell Sci 113(19):3473–3484
Fawcett DW (1966) On the occurrence of a fibrous lamina on the inner aspect of the nuclear envelope in certain cells of vertebrates. Am J Anat 119:129–145
Feron O, Smith TW, Michel T, Kelly RA (1997) Dynamic targeting of the agonist-stimulated m2 muscarinic acetylcholine receptor to caveolae in cardiac myocytes. J Biol Chem 272(28):17744–17748
Goodpasture C, Bloom SE (1975) Visualization of nucleolar organizer regions in mammalian chromosomes using silver staining. Chromosoma 20:37–50
Guasch RM, Guerri C, Oconnor JE (1995) Study of surface carbohydrates on isolated Golgi subfractions by fluorescent lectin-binding and flow-cytometry. Cytometry 19:112–118
Hagiwara H, Tajika Y, Matsuzaki T, Suzuki T, Aoki T, Takata K (2006) Localization of Golgi 58 K protein (formiminotransferase cyclodeaminase) to the centrosome. Histochem Cell Biol 126:251–259
Li J, Sejas DP, Burma S, Chen DJ, Pang Q (2007) Nucleophosmin suppresses oncogene-induced apoptosis and senescence and enhances oncogenic cooperation in cells with genomic instability. Carcinogenesis 28:1163–1170
Lojda Z, Gossrau R, Schiebler T (1976) Enzyme histochemistry. A laboratory manual. Springer, Berlin
Moll R, Franke WW, Schiller DL (1982) The catalog of human cytokeratins: patterns of expression in normal epithelia, tumors and cultured cells. Cell 31:11–24
Moll R, Löwe A, Laufer J, Franke WW (1992) Cytokeratin 20 in human carcinomas. A new histodiagnostic marker detected by monoclonal antibodies. Am J Pathol 140:427–447
Ploton D, Menager M, Jeannesson P, Himber G, Pigeon F, Adnet JJ (1986) Improvement in the staining and in the visualization of the argyrophilic proteins of the nucleolar organizer region at the optical level. Histochem J 18:5–14
Polak JM, Van Noorden S (1997) Introduction to immunocytochemistry. BIOS Scientific Publishers, Oxford
Poot M, Zhang YZ, Kramer JA, Wells KS, Jones LJ, Hanzel DK, Lugade AG, Singer VL, Haugland RP (1996) Analysis of mitochondrial morphology and function with novel fixable fluorescent stains. J Histochem Cytochem 44:1363–1372
Schweizer J, Bowden PE, Coulombe PA, Langbein L, Lane EB, Magin TM, Maltais L, Omary MB, Parry DA, Rogers MA, Wright MW (2006) New consensus nomenclature for mammalian keratins. J Cell Biol 174(2):169–174
Treré D (2000) AgNOR staining and quantification. Micron 31:127–131
Ugrinova I, Monier K, Ivaldi C, Thiry M, Storck S, Mongelard F, Bouvet P (2007) Inactivation of nucleolin leads to nucleolar disruption, cell cycle arrest and defects in centrosome duplication. BMC Mol Biol 8:66
Valdez BC, Perlaky L, Saijo Y, Henning D, Zhu C, Busch RK, Zhang WW, Busch H (1992) A region of antisense RNA from human p120 cDNA with high homology to mouse p120 cDNA inhibits NIH 3 T3 proliferation. Cancer Res 52(20):5681–5686
Van Noorden CJF, Frederiks WM (1993) Enzyme Histochemistry: A Laboratory Manual of Current Methods. Bios Scientific Publishers Ltd., Oxford
Virtanen I, Ekblom P, Laurila P (1980) Subcellular compartmentalization of saccharide moieties in cultured normal and malignant-cells. J Cell Biol 85:429–434
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
Copyright information
© 2010 Springer-Verlag Berlin Heidelberg
About this chapter
Cite this chapter
Buchwalow, I.B., Böcker, W. (2010). Probes for Staining Specific Cellular Organelles. In: Immunohistochemistry: Basics and Methods. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-04609-4_10
Download citation
DOI: https://doi.org/10.1007/978-3-642-04609-4_10
Published:
Publisher Name: Springer, Berlin, Heidelberg
Print ISBN: 978-3-642-04608-7
Online ISBN: 978-3-642-04609-4
eBook Packages: Biomedical and Life SciencesBiomedical and Life Sciences (R0)