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Localization of Golgi 58K protein (formiminotransferase cyclodeaminase) to the centrosome

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Abstract

In vertebrate cells, the centrosome consists of a pair of centrioles and surrounding pericentriolar material. Using anti-Golgi 58K protein antibodies that recognize formiminotransferase cyclodeaminase (FTCD), we investigated its localization to the centrosome in various cultured cells and human oviductal secretory cells by immunohistochemistry. In addition to the Golgi apparatus, FTCD was localized to the centrosome, more abundantly around the mother centriole. The centrosome localization of FTCD continued throughout the cell cycle and was not disrupted after Golgi fragmentation, which was induced by colcemid and brefeldin A. Centriole microtubules are polyglutamylated and stable against tubulin depolymerizing drugs. FTCD in the centrosome may be associated with polyglutamylated residues of centriole microtubules and may play a role in providing centrioles with glutamate produced by cyclodeaminase domains of FTCD.

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Acknowledgements

This work was supported in part by Grants-in-aid for Scientific Research from the Ministry of Education, Culture, Science and Technology of Japan.

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Authors and Affiliations

  1. Department of Anatomy and Cell Biology, Gunma University Graduate School of Medicine, 3-39-22, Showa-machi, Maebashi, Gunma, 371-8511, Japan

    Haruo Hagiwara, Yuki Tajika, Toshiyuki Matsuzaki, Takeshi Suzuki, Takeo Aoki & Kuniaki Takata

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  1. Haruo Hagiwara
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  2. Yuki Tajika
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  3. Toshiyuki Matsuzaki
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  4. Takeshi Suzuki
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  5. Takeo Aoki
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  6. Kuniaki Takata
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Correspondence to Haruo Hagiwara.

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Hagiwara, H., Tajika, Y., Matsuzaki, T. et al. Localization of Golgi 58K protein (formiminotransferase cyclodeaminase) to the centrosome. Histochem Cell Biol 126, 251–259 (2006). https://doi.org/10.1007/s00418-006-0166-5

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