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Isolation and Growth of Stem Cells

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Tissue Engineering

Abstract

Multiple tissues can serve as a source of adult or somatic stem cells. Some of these tissues are available at only one point in the lifecycle, such as the umbilical cord, Wharton’s jelly, and placenta. In contrast, others are available throughout life and these include adipose tissue, bone marrow, and skeletal muscle. This chapter focuses on the latter three tissues due to their availability and utility for autologous and allogeneic transplantation. While none of these tissues yields a “perfect” stem cell, they do exhibit some of the following ideal properties: Abundant, accessible, and replenishable tissue source Multiple lineage differentiation potential Nontumorigenic Suitable for both autologous and allogeneic transplant Capable of retaining the desired properties after long-term storage Available in quantities of billions of cells Low immunogenicity Limited donor to donor variability Simple and reproducible isolation procedure

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Author information

Authors and Affiliations

  1. Pennington Biomedical Research Center, LSU System, 6400 Perkins Road, Baton Rouge, LA, 70808, USA

    Jeffrey M. Gimble

  2. Tulane Center for Stem Cell Research and Regenerative Medicine, Tulane University Health Sciences Center, J. Bennett Johnston Building, 1324 Tulane Avenue, SL-99, New Orleans, LA, 70112-2699, USA

    Bruce A. Bunnell

  3. Department of Orthopaedic Surgery, Division of Orthopedics, Duke University Medical Center, Box 3093, Durham, NC, 27710, USA

    Farshid Guilak

  4. Sanford Burnham Medical Research Institute, 2566 Lee Rd, Winter Park, Florida, 32789, USA

    Steven R. Smith (Scientific Director Translational Research Institute for Metabolism and Diabetes Florida Hospital)

  5. Department of Plastic and Maxillofacial Surgery, University of Virginia, West Complex, 1335 Lee Street, Charlottesville, VA, 22908, USA

    Adam J. Katz

Authors
  1. Jeffrey M. Gimble
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  2. Bruce A. Bunnell
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  3. Farshid Guilak
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  4. Steven R. Smith
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  5. Adam J. Katz
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Corresponding author

Correspondence to Jeffrey M. Gimble .

Editor information

Editors and Affiliations

  1. Klinik für Plastische Chirurgie,, Hand- und Verbrennungschirurgie, Universitätsklinikum Aachen, Pauwelsstr. 30, Aachen, 52057, Germany

    Norbert Pallua

  2. Klinik für Plastische Chirurgie,, Hand- und Verbrennungschirurgie, Universitätsklinikum Aachen, Pauwelsstr. 30, Aachen, 52057, Germany

    Christoph V. Suscheck

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Gimble, J.M., Bunnell, B.A., Guilak, F., Smith, S.R., Katz, A.J. (2011). Isolation and Growth of Stem Cells. In: Pallua, N., Suscheck, C. (eds) Tissue Engineering. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-02824-3_6

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