Abstract
We present a technology of culturing of human mesenchymal stem cells under conditions excluding the presence of animal sera or additional growth factors, but preserving high proliferative potential and the capacity of these cells to multilineage differentiation. Human umbilical serum was used as the alternative material. We found that in the presence of human umbilical serum mesenchymal stem cells more effectively proliferate and retain their differentiation capacity. The proposed technology yields 109–1010 morphologically and functionally identical cells.
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Translated from Kletochnye Tekhnologii v Biologii i Medicine, No. 4, pp. 206–211, December, 2006
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Romanov, Y.A., Darevskaya, A.N., Kabaeva, N.V. et al. Optimum conditions for culturing of human bone marrow and adipose tissue mesenchymal precursor cells. Bull Exp Biol Med 142, 515–520 (2006). https://doi.org/10.1007/s10517-006-0406-7
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DOI: https://doi.org/10.1007/s10517-006-0406-7