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New Insights Into Leukocyte Recruitment by Intravital Microscopy

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Visualizing Immunity

Part of the book series: Current Topics in Microbiology and Immunology ((CT MICROBIOLOGY,volume 334))

Abstract

Leukocyte recruitment to sites of inflammation requires adhesion to and transmigration through the blood vessel wall. Recent progress in optical equipment and new genetic and molecular tools have revealed additional steps in the leukocyte adhesion cascade beyond rolling, adhesion, and transmigration. In vivo studies using intravital microscopy (IVM) were essential for the discovery of slow rolling, postadhesion strengthing, intraluminal crawling, and different routes of transmigration. IVM revealed unique features of leukocyte recruitment in different organs. This review focuses on insights into the leukocyte adhesion cascade gained by IVM.

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Acknowledgments

Supported by grants from Deutsche Forschungsgemeinschaft (AZ 428/2–1 and AZ 428/3-1 to A.Z.) and by grants from the National Institutes of Health to K.L.

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  1. Department of Anesthesiology and Intensive Care Medicine, University of Münster, Münster, Germany

    Alexander Zarbock

  2. La Jolla Institute for Allergy and Immunology, La Jolla, CA, USA

    Alexander Zarbock & Klaus Ley

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    Zarbock, A., Ley, K. (2009). New Insights Into Leukocyte Recruitment by Intravital Microscopy . In: Dustin, M., McGavern, D. (eds) Visualizing Immunity. Current Topics in Microbiology and Immunology, vol 334. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-540-93864-4_6

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