Abstract
Serial block face scanning electron microscopy (SBFSEM) is a new technique designed to obtain image slices through specimens inside a scanning electron microscope (SEM) in an automated process. Biological tissue in particular, is prepared by classical en-block staining and resin embedding as used for many years for transmission electron microscopy.
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Genoud, C., Mancuso, J., Monteith, S., Kraus, B. (2008). Automatic acquisition of large amounts of 3D data at the ultrastructural level, using serial block face scanning electron microscopy. In: Luysberg, M., Tillmann, K., Weirich, T. (eds) EMC 2008 14th European Microscopy Congress 1–5 September 2008, Aachen, Germany. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-540-85156-1_274
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DOI: https://doi.org/10.1007/978-3-540-85156-1_274
Publisher Name: Springer, Berlin, Heidelberg
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