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Electrospun Collagen Scaffolds

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Electrospun Biomaterials and Related Technologies

Abstract

Nanofibrous collagen scaffolds developed via electrospinning have revolutionized the field of designing useful biomaterials for regenerative or tissue engineering. Electrospun collagen scaffolds allow for the replication of the extracellular matrix of tissues with regards of their chemical, physical, and mechanical characteristics. Because collagen is the most abundant protein found in tissues, it can be the base for an ideal scaffold to mimic the majority of soft or hard tissues such as bone, which contains an organic component composed of collagen and a mineral component. The physical and mechanical properties of the collagen nanofibers are of vital importance to promote the necessary and specific signals of the cellular or tissue environment supporting different cellular processes. This chapter describes the fabrication and modulation of the physical and mechanical properties of electrospun collagen nanofibers and their biomedical applications.

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Abbreviations

AcOH:

Dilute acetic acid

AFM:

Atomic force microscopy

BDDGE:

1,4-Butanediol diglycidyl ether

CD:

Circular dichroism spectroscopy

Col/PCL:

Collagen/polycaprolactone

DMSO:

Dimethyl sulfoxide

ECs:

Endothelial cells

ECM:

Extracellular matrix

EDC:

1-Ethyl-3-(3-dimethyl-aminopropyl)-1-carbodiimide hydrochloride

EtOH:

Ethanol

FTIR:

Fourier transform infrared spectroscopy

GAG:

Glycosaminoglycan

GFAP staining:

Glial fibrillary acidic protein staining

HA:

Nanohydroxyapatite

HAc:

Acetic acid

HFIP:

Hexafluoroisopropanol

HFP:

1,1,1,3,3,3-Hexafluoro-2-propanol

MSCs:

Mesenchymal stem cells

NHOK:

Normal human oral keratinocytes

NHS:

N-Hydroxysuccinimide

NOI:

Normalized orientation index

NRVCM:

Ventricular cardiomyocytes of primary neonatal rats

PHBV:

Poly(3-hydroxybutyrate-co-3-hydroxyvalerate)

PIECs:

Porcine iliac artery endothelial cells

PLGA:

Poly(lactide-co-glycolide

PLLA:

Poly(l-lactic acid)

SCs:

Schwann cells

SCI:

Spinal cord injury

SEM:

Scanning electron microscope

SMCs:

Smooth muscle cells

TFE:

Tri-fluoroethanol

TPU:

Thermoplastic polyurethane

vWF:

Von Willebrand factor

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Authors and Affiliations

  1. Department of Chemical Engineering, University of Puerto Rico Mayaguez, Mayaguez, PR, USA

    David A. Castilla-Casadiego & Jorge Almodóvar

  2. Bioengineering Graduate Program, University of Puerto Rico Mayaguez, Mayaguez, PR, USA

    Carol A. Rivera-Martínez & Jorge Almodóvar

  3. Department of Biology, University of Puerto Rico Mayaguez, Mayaguez, PR, USA

    Beatriz A. Quiñones-Colón

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  1. David A. Castilla-Casadiego
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  2. Carol A. Rivera-Martínez
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  3. Beatriz A. Quiñones-Colón
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  4. Jorge Almodóvar
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Correspondence to Jorge Almodóvar .

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  1. Department of Chemical Engineering, University of Puerto Rico Mayaguez, Mayaguez, Puerto Rico, Puerto Rico

    Jorge Almodovar

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Castilla-Casadiego, D.A., Rivera-Martínez, C.A., Quiñones-Colón, B.A., Almodóvar, J. (2017). Electrospun Collagen Scaffolds. In: Almodovar, J. (eds) Electrospun Biomaterials and Related Technologies. Springer, Cham. https://doi.org/10.1007/978-3-319-70049-6_2

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