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Electronic-Automated Micro-NMR Assay with DMF Device

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Handheld Total Chemical and Biological Analysis Systems

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Abstract

We describe a micro-NMR relaxometer miniaturized into palm size and electronic-automated for multistep multi-sample chemical/biological diagnosis. The co-integration of microfluidic and microelectronic technologies enables association between droplet managements and micro-NMR assays inside a portable sub-Tesla magnet (1.2 kg, 0.46 Tesla). Targets captured by specific probe-decorated magnetic nanoparticles can be sequentially quantified by their spin-spin relaxation time via multiplexed micro-NMR screening. Distinct droplet samples are operated by a digital microfluidic device that electronically manages the electrowetting-on-dielectric effects over an electrode array. Each electrode (3.5 × 3.5 mm2) is scanned with capacitive sensing to locate distinct droplet samples in real time. A cross-domain-optimized Butterfly-coil-input semiconductor transceiver transduces between magnetic and electrical signals to/from a sub-10 μL droplet sample for high-sensitivity micro-NMR screening. We have implemented two prototypes. The first prototype was implemented with discrete electronics for verification of functionality, while the second prototype was designed with a CMOS TRX for better performance. Fabricated in 0.18-μm CMOS, the TRX occupies a die area of 2.1 mm2, consumes 6.6/23.7 mW of power in the TX/RX mode, and demonstrates the feasibility of electronic-automated biological (avidin) and chemical (CuSO4) assays achieving a detection limit on avidin of 0.2 pmol.

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Authors and Affiliations

  1. State-Key Laboratory of Analog and Mixed-Signal VLSI, University of Macau, Macau, China

    Ka-Meng Lei & Man-Kay Law

  2. State-Key Laboratory of Analog and Mixed-Signal VLSI and FST-ECE, University of Macau, Macau, China

    Pui-In Mak & Rui Paulo Martins

  3. Instituto Superior Técnico Universidade de Lisboa, Lisbon, Portugal

    Rui Paulo Martins

Authors
  1. Ka-Meng Lei
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  2. Pui-In Mak
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  3. Man-Kay Law
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  4. Rui Paulo Martins
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Lei, KM., Mak, PI., Law, MK., Martins, R.P. (2018). Electronic-Automated Micro-NMR Assay with DMF Device. In: Handheld Total Chemical and Biological Analysis Systems. Springer, Cham. https://doi.org/10.1007/978-3-319-67825-2_3

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  • DOI: https://doi.org/10.1007/978-3-319-67825-2_3

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  • Publisher Name: Springer, Cham

  • Print ISBN: 978-3-319-67824-5

  • Online ISBN: 978-3-319-67825-2

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