Skip to main content

Gene Annotation and Database of the Radish

  • Chapter
  • First Online:
The Radish Genome

Part of the book series: Compendium of Plant Genomes ((CPG))

  • 1097 Accesses

Abstract

The genome sequences of Raphanus sativus var. hortensis were determined for the Japanese cultivars ‘Aokubi S-h’ (Kitashiba et al. in DNA Res 21:481–490, 2014), ‘Aokubi doubled haploid (DH)’ (Mitsui et al. in Sci Rep 5:10835, 2015), and the Korean cultivar ‘WK10039’ (Jeong et al. in Theor Appl Genet 129:1357–1372, 2016). After the genome sequencing, gene annotations , such as gene finding, domain searches, and similarity searches against several databases , were conducted. The genomic information obtained by these analyses was then released from a database. In this section, the gene annotation methods conducted in each of the three genome sequencing projects is described. In addition, several databases related to the genome sequencing, EST sequencing, and DNA markers in radish are introduced.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

eBook
USD 16.99
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 119.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD 169.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

References

  • Altschul SF, Madden TL, Schäffer AA, Zhang J, Zhang Z, Miller W, Lipman DJ (1997) Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. Nucleic Acids Res 25:3389–3402

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Asamizu E, Ichihara H, Nakaya A, Nakamura Y, Hirakawa H, Ishii T, Tamura T, Fukami-Kobayashi K, Nakajima Y, Tabata S (2014) Plant Genome DataBase Japan (PGDBj): a portal website for the integration of plant genome-related databases. Plant Cell Physiol 55:e8

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Conesa A, Götz S, García-Gómez JM, Terol J, Talón M, Robles M (2005) Blast2GO: a universal tool for annotation, visualization and analysis in functional genomics research. Bioinformatics 21:3674–3676

    Article  CAS  PubMed  Google Scholar 

  • Eddy SR (2011) Accelerated profile HMM searches. PLoS Comput Biol 7:e1002195

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Grabherr MG, Haas BJ, Yassour M, Levin JZ, Thompson DA, Amit I, Adiconis X, Fan L, Raychowdhury R, Zeng Q, Chen Z, Mauceli E, Hacohen N, Gnirke A, Rhind N, di Palma F, Birren BW, Nusbaum C, Lindblad-Toh K, Friedman N, Regev A (2011) Full-length transcriptome assembly from RNA-Seq data without a reference genome. Nat Biotechnol 29:644–652

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Haas B, Salzberg S, Zhu W, Pertea M, Allen J, Orvis J, White O, Buell C, Wortman J (2008) Automated eukaryotic gene structure annotation using EVidenceModeler and the program to assemble spliced alignments. Genome Biol 9:R7

    Article  PubMed  PubMed Central  Google Scholar 

  • Jeong YM, Kim N, Ahn BO, Oh M, Chung WH, Chung H, Jeong S, Lim KB, Hwang YJ, Kim GB, Baek S, Choi SB, Hyung DJ, Lee SW, Sohn SH, Kwon SJ, Jin M, Seol YJ, Chae WB, Choi KJ, Park BS, Yu HJ, Mun JH (2016) Elucidating the triplicated ancestral genome structure of radish based on chromosome-level comparison with the Brassica genomes. Theor Appl Genet 129:1357–1372

    Article  CAS  PubMed  Google Scholar 

  • Kitashiba H, Li F, Hirakawa H, Kawanabe T, Zou Z, Hasegawa Y, Tonosaki K, Shirasawa S, Fukushima A, Yokoi S, Takahata Y, Kakizaki T, Ishida M, Okamoto S, Sakamoto K, Shirasawa K, Tabata S, Nishio T (2014) Draft sequences of the radish (Raphanus sativus L.) genome. DNA Res 21:481–490

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Korf I (2004) Gene finding in novel genomes. BMC Bioinformat 5:59

    Article  Google Scholar 

  • Li L, Stoeckert CJ Jr, Roos DS (2003) OrthoMCL: identification of ortholog groups for eukaryotic genomes. Genome Res 13:2178–2189

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Li W, Godzik A (2006) Cd-hit: a fast program for clustering and comparing large sets of protein or nucleotide sequences. Bioinformatics 22:1658–1659

    Article  CAS  PubMed  Google Scholar 

  • Llorens C, Futami R, Covelli L, Domínguez-Escribá L, Viu JM, Tamarit D, Aguilar-Rodríguez J, Vicente-Ripolles M, Fuster G, Bernet GP, Maumus F, Munoz-Pomer A, Sempere JM, Latorre A, Moya A (2011) The Gypsy Database (GyDB) of mobile genetic elements: release 2.0. Nucleic Acids Res 39(Database Issue):D70–D74

    Article  CAS  PubMed  Google Scholar 

  • Lowe TM, Eddy SR (1997) tRNAscan-SE: a program for improved detection of transfer RNA genes in genomic sequence. Nucleic Acids Res 25:955–964

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Mitsui Y, Shimomura M, Komatsu K, Namiki N, Shibata-Hatta M, Imai M, Katayose Y, Mukai Y, Kanamori H, Kurita K, Kagami T, Wakatsuki A, Ohyanagi H, Ikawa H, Minaka N, Nakagawa K, Shiwa Y, Sasaki T (2015) The radish genome and comprehensive gene expression profile of tuberous root formation and development. Sci Rep 5:10835

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Moghe GD, Hufnagel DE, Tang H, Xiao Y, Dworkin I, Town CD, Conner JK, Shiu SH (2014) Consequences of whole-genome triplication as revealed by comparative genomic analyses of the wild radish Raphanus raphanistrum and three other Brassicaceae species. Plant Cell 26:1925–1937

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Mulder NJ, Apweiler R, Attwood TK, Bairoch A, Bateman A, Binns D, Bork P, Buillard V, Cerutti L, Copley R, Courcelle E, Das U, Daugherty L, Dibley M, Finn R, Fleischmann W, Gough J, Haft D, Hulo N, Hunter S, Kahn D, Kanapin A, Kejariwal A, Labarga A, Langendijk-Genevaux PS, Lonsdale D, Lopez R, Letunic I, Madera M, Maslen J, McAnulla C, McDowall J, Mistry J, Mitchell A, Nikolskaya AN, Orchard S, Orengo C, Petryszak R, Selengut JD, Sigrist CJ, Thomas PD, Valentin F, Wilson D, Wu CH, Yeats C (2007) New developments in the InterPro database. Nucleic Acids Res 35(Database Issue):D224–D228

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Ogata H, Goto S, Sato K, Fujibuchi W, Bono H, Kanehisa M (1999) KEGG: Kyoto encyclopedia of genes and genomes. Nucleic Acids Res 27:29–34

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Quevillon E, Silventoinen V, Pillai S, Harte N, Mulder N, Apweiler R, Lopez R (2005) InterProScan: protein domains identifier. Nucleic Acids Res 33(Web Server Issue):W116–W120

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Rice P, Longden I, Bleasby A (2000) EMBOSS: the European molecular biology open software suite. Trends Genet 16:276–277

    Article  CAS  PubMed  Google Scholar 

  • Shen D, Sun H, Huang M, Zheng Y, Li X, Fei Z (2013) RadishBase: a database for genomics and genetics of radish. Plant Cell Physiol 54:e3

    Article  CAS  PubMed  Google Scholar 

  • Shirasawa K, Isobe S, Tabata S, Hirakawa H (2014) Kazusa marker DataBase: a database for genomics, genetics, and molecular breeding in plants. Breed Sci 64:264–271

    Article  PubMed  PubMed Central  Google Scholar 

  • Stanke M, Waack S (2003) Gene prediction with a hidden Markov model and a new intron submodel. Bioinformatics 19:ii215–ii225

    Article  PubMed  Google Scholar 

  • Tatusov RL, Fedorova ND, Jackson JD, Jacobs AR, Kiryutin B, Koonin EV, Krylov DM, Mazumder R, Mekhedov SL, Nikolskaya AN, Rao BS, Smirnov S, Sverdlov AV, Vasudevan S, Wolf YI, Yin JJ, Natale DA (2003) The COG database: an updated version includes eukaryotes. BMC Bioinformat 4:41

    Article  Google Scholar 

  • Wu T, Nacu N (2010) Fast and SNP-tolerant detection of complex variants and splicing in short reads. Bioinformatics 26:873–881

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Zhang X, Yue Z, Mei S, Qiu Y, Yang X, Chen X, Cheng F, Wu Z, Sun Y, Jing Y, Liu B, Shen D, Wang H, Cui N, Duan Y, Wu J, Wang J, Gan C, Wang J, Wang X, Li X (2015) A de novo genome of a Chinese radish cultivar. Hortic Plant J 1:155–164

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to Hideki Hirakawa .

Editor information

Editors and Affiliations

Rights and permissions

Reprints and permissions

Copyright information

© 2017 Springer International Publishing AG

About this chapter

Cite this chapter

Hirakawa, H. (2017). Gene Annotation and Database of the Radish. In: Nishio, T., Kitashiba, H. (eds) The Radish Genome. Compendium of Plant Genomes. Springer, Cham. https://doi.org/10.1007/978-3-319-59253-4_4

Download citation

Publish with us

Policies and ethics