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Flow Cytometric Methods for Circulating Tumor Cell Isolation and Molecular Analysis

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Isolation and Molecular Characterization of Circulating Tumor Cells

Part of the book series: Advances in Experimental Medicine and Biology ((AEMB,volume 994))

Abstract

Circulating tumor cells provide a non-invasive source of tumor material that can be valuable at all stages of disease management, including screening and early diagnosis, monitoring response to therapy, identifying therapeutic targets, and assessing development of drug resistance. Cells isolated from the blood of cancer patients can be used for phenotypic analysis, tumor genotyping, transcriptional profiling, as well as for ex vivo culture of isolated cells. There are a variety of novel technologies currently being developed for the detection and analysis of rare cells in circulation of cancer patients. Flow cytometry is a powerful cell analysis platform that is increasingly being used in this field of study due to its relatively high throughput and versatility with respect to the large number of commercially available antibodies and fluorescent probes available to translational and clinical researchers. More importantly, it offers the ability to easily recover viable cells with high purity that are suitable for downstream molecular analysis, thus making it an attractive technology for cancer research and as a diagnostic tool.

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Authors and Affiliations

  1. Division of Gastroenterology, Department of Medicine, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, 19104, USA

    Neha Bhagwat

  2. Division of Hematology/Oncology, Department of Medicine, Perelman School of Medicine, Abramson Cancer Center, University of Pennsylvania, Philadelphia, PA, 19104, USA

    Erica L. Carpenter

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  1. Neha Bhagwat
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Correspondence to Erica L. Carpenter .

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Editors and Affiliations

  1. University of California San Francisco, San Francisco, California, USA

    Mark Jesus M. Magbanua

  2. University of California San Francisco, San Francisco, California, USA

    John W. Park

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Bhagwat, N., Carpenter, E.L. (2017). Flow Cytometric Methods for Circulating Tumor Cell Isolation and Molecular Analysis. In: Magbanua, M., Park, J. (eds) Isolation and Molecular Characterization of Circulating Tumor Cells. Advances in Experimental Medicine and Biology, vol 994. Springer, Cham. https://doi.org/10.1007/978-3-319-55947-6_5

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