Abstract
Stable isotope labelling by amino acids in cell culture (SILAC) is a technique that allows proteomic profiling of cells. In this chapter we describe a protocol for the identification and quantification of newly synthesised proteins. The methodology can be applied to any cultured cell system with relevance to schizophrenia, affective disorders and autism spectrum conditions including those addressing responses to pharmacological stimuli.
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Acknowledgements
DDG was the recipient of a FAPESP post-doctoral fellowship (BEPE-PD 2014/20380-5) and is funded by the BHF (PG/14/20/30769). JdAF was the recipient of a FAPESP doctoral fellowship (BEPE-DR 2014/17012-4); TPO was a recipient of a Visiting Scientist CAPES Science Without Borders fellowship (BEX 11766-13-1). SEO is funded by the Medical Research Council (MC_UU_12012/4).
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Duque-Guimarães, D., Ong, T.P., de Almeida-Faria, J., Guest, P.C., Ozanne, S.E. (2017). SILAC Mass Spectrometry Profiling: A Psychiatric Disorder Perspective. In: Guest, P. (eds) Proteomic Methods in Neuropsychiatric Research. Advances in Experimental Medicine and Biology(), vol 974. Springer, Cham. https://doi.org/10.1007/978-3-319-52479-5_27
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DOI: https://doi.org/10.1007/978-3-319-52479-5_27
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